GSH/GSSG檢測套組 | GSH/GSSG Ratio Assay Sciencell貨號8558
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GSH/GSSG檢測套組 | GSH/GSSG Ratio Assay Sciencell貨號8558
偵測範圍0.195-6.25 uM
穀胱甘肽(Glutathione) 是一種三肽(tripeptide)（g-glutamylcysteinylglycine , g-谷氨酰半胱氨酰甘氨酸），以還原（reduced GSH）和氧化（GSSG）狀態存在。穀胱甘肽過氧化物酶催化由 GSH 形成 GSSG，穀胱甘肽還原酶將 GSSG 再循環為 GSH。隨著氧化應激(oxidative stress)水平的增加，細胞內 GSSG 會積累，GSH/GSSG 比率會降低。因此，測定 GSH/GSSG 比值是細胞和組織中氧化應激的有用指標。這種比色測定基於 5′, 5′-二硫代雙 2-硝基苯甲酸 (DTNB) 和 GSH 之間的反應形成 TNB，其在 412 nm 處表現出最大吸光度。吸光度的強度與樣品中的 GSH 水平成正比。GSH/GSSG 比率測定試劑盒可測量不同類型樣品（如組織、細胞裂解物和血漿）中 GSH 和 GSSG 之間的比率。本產品僅用於研究目的，不得用於動物、人類或診斷程序。
GSH/GSSH產品內容物
GSH/GSSHvfgr wd qmv mgbuu
GSH/GSSH原理及濃度計算
Glutathione is a tripeptide (g-glutamylcysteinylglycine) and exists in reduced (GSH) and oxidized (GSSG) states。Glutathione peroxidase catalyzes the formation of GSSG from GSH, and glutathione reductase recycles GSSG to GSH. With increased levels of oxidative stress, intracellular GSSG will accumulate, and the GSH/GSSG ratio will decrease. Therefore, the determination of the GSH/GSSG ratio is a useful indicator of oxidative stress in cells and tissues. This colorimetric assay is based on the reaction between 5’, 5’-Dithiobis 2- nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the GSH level in the sample。
| Catalog No. | 8558 |
| Country of Manufacture | United States |
| Product Code | GSH/GSSG |
| Size/Quantity | 100 Tests |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store in -20°C |
| Shipping Info | Dry ice. |
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| 8118 | MGPA | Malachite Green Phosphate Assay |
| 8128 | CCA | Colorimetric Calcium Assay |
| 8308 | LAC | L-Lactate Assay |
| 8338 | GLU | Glutamate Assay |
| 8368 | NAD | NAD/NADH Assay |
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| 8518 | TGA | Total Glutathione Assay |
| 8528 | AA | Ammonia Assay |
| 8558 | GSH/GSSG | GSH/GSSG Ratio Assay |
| 8588 | Cho | Choline Assay |
| 8648 | Mal | Malate Assay |