【Lysozyme溶菌酶檢測】溶菌酶活性分析/溶菌酶抑制劑篩選試劑盒Abcam (Biovision)
【溶菌酶檢測】溶菌酶活性分析/溶菌酶抑制劑篩選試劑盒/溶菌酶抗體-Abcam (Biovision)
溶菌酶（Lysozyme, EC 3.2.1.17），也稱為胞壁質酶(muramidase)或N-乙酰胞壁酰胺聚醣水解酶 (N-acetylmuramide glycanhydrolase) ，是作用於糖苷鍵(glycosidic bonds) 的水解酶。它水解粘多醣細胞壁中存在的N-乙酰基 – 胞嘧啶酸和N-乙酰基-D-葡糖胺殘基之間的β-（1-4） – 葡糖苷鍵 (β-(1-4)-glucosidic linkage)。溶菌酶(Lysozyme)廣泛存在於各種生物液體中，如淚液(tears)，唾液(saliva)和組織(tissues)，它也存在於巨噬細胞的細胞質顆粒和多形核中性粒細胞（PMN）中。它具有抗菌(antibacterial)，抗腫瘤(antitumor)和免疫調節活性(immune modulatory)。據報導，在患有白血病(leukemia)，結核病(tuberculosis)，巨幼紅細胞性貧血(megaloblastic anemias)，急性細菌感染(acute bacterial infections)，潰瘍性結腸炎(ulcerative colitis)，嚴重腎功能不全(renal insufficiency)，腎盂腎炎(pyelonephritis)和腎炎(nephritis)的患者中，尿液(urine)和血清(serum)中溶菌酶(Lysozyme)的濃度升高。
溶菌酶活性檢測試劑盒（螢光） | 貨號K236-100Lysozyme Activity Assay Kit (Fluorometric)
產品描述：BioVision的溶菌酶活性測定試劑盒利用溶菌酶裂解合成底物的能力，釋放出易於定量的游離螢光團（Ex / Em = 360 /445 nm）。該試劑盒提供簡單，超靈敏的檢測方法，可在多種生物樣品中檢測低至2μU/ ml的Lysozyme溶菌酶活性。
| Cat # +Size | K236-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 360/445 nm) |
| Applications | Detection of Lysozyme Activity in variety of samples |
| Features & Benefits | • Rapid, and convenient • The assay can detect Lysozyme activity as low as 2 µU/ml in a variety of biological samples |
| Kit Components | • Lysozyme Assay Buffer • Lysozyme Stop Buffer • Lysozyme Substrate (in DMSO) • Lysozyme Positive Control (lyophilized) • 4-Methylumbelliferone Standard (5 mM) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
Lysozyme | CAS#103476-89-7貨號M1237
產品介紹: Lysozyme 是一種糖苷水解酶(glycoside hydrolase,它能水解 1,4-β-連接(1,4-beta-linkages)在苯乙醯穆拉酸(N-acetylmuramic acid)和 N-乙醯-D-葡萄糖胺殘留物(N-acetyl-D-glucosamine residues)在多吉多解酶和 N-乙醯D-葡萄糖胺殘留物在膽鹼(chitodextrin)中。Peptidoglycan是革蘭式陽性細菌細胞壁(gram-positive bacteria)的主要成分,這使得這些細菌容易被淋索酶菌所感染。它用於過濾細菌和製備球菌。從蛋清(chicken egg white)純化並冷凍乾燥。
| Highlights | Source: Chicken Egg White (Enzymatic Grade) Activity: ~50, 000 U/mg CAS #: 12650-88-3 Appearance: Freeze-dried powder Purity: 95% (biuret) Solubility: Soluble in water Applications: It is used to lyse bacteria and to prepare spheroplasts |
| Storage Conditions | -20ºC |
| Shipping Conditions | RT |
| USAGE | For Research Use Only! Not For Use in Humans. |
Lysozyme ELISA Kit
產品描述：Lysozyme溶菌酶（EC 3.2.1.17），也稱為muramidase胞壁質酶或N-乙酰胞壁酰胺聚醣水解酶 (N-acetylmuramide glycanhydrolase) ，是作用於糖苷鍵(glycosidic bonds) 的水解酶。它水解粘多醣細胞壁中存在的N-乙酰基 – 胞嘧啶酸和N-乙酰基-D-葡糖胺殘基之間的β-（1-4） – 葡糖苷鍵 (β-(1-4)-glucosidic linkage)。Lysozyme溶菌酶廣泛存在於各種生物液體中，如tears淚液，saliva唾液和tissues組織。它也存在於巨噬細胞的細胞質顆粒和多形核中性粒細胞（PMN）中。 BioVision的Lysozyme ELISA試劑盒是一種夾心ELISA檢測，用於定量測量血清，血漿和生物液中的人溶菌酶。 顏色密度與從樣品中捕獲的人溶菌酶的量成比例。
| Cat # +Size | K4243-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | This ELISA kit is used for quantitative measurement of Lysozyme in human serum, plasma and other biological fluids. |
| Features & Benefits |
• Easy, convenient and time-saving method to measure the level of Lysozyme in human serum, plasma and other biological fluids. • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. |
| Kit Components | • Micro ELISA Plate • Lysozyme Standards • Lysozyme Controls • Anti-Lysozyme Enzyme Conjugate • Sample Diluent • TMB Substrate • Wash Concentrate (20X) • Stop Solution |
Lysozyme Inhibitor Screening Kit
產品描述：BioVision的溶菌酶抑制劑篩選試劑盒使用N，N’，N” – Triacetylchitotriose-一種競爭性抑制劑，可與溶菌酶的活性位點結合。該抑制劑降低了溶菌酶對水解螢光底物的催化能力，提供簡單，快速，靈敏和可靠的測試，適用於篩選溶菌酶抑制劑。
| Cat # +Size | K237-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 360/445 nm) |
| Species Reactivity | Mammalian |
| Applications | The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Lysozyme inhibitors. Inhibitor control (N,N’,N”-Triacetylchitotriose) is included to compare the efficacy of test inhibitors. |
| Features & Benefits | • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable |
| Kit Components | • Lysozyme Assay Buffer • Lysozyme substrate (in DMSO) • Lysozyme (lyophilized) • N,N’,N”-Triacetylchitotriose |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Lysozyme Antibody
產品描述：Lysozymes溶菌酶主要具有bacteriolytic function溶菌功能; 組織和體液中的那些與單核細胞 – 巨噬細胞系統相關並增強免疫試劑的活性。 它具有與天然溶菌酶相同的氨基酸序列和物理化學性質。 該酶切割細菌細胞壁中肽聚醣組分的N-乙酰葡糖胺和N-乙酰胞壁酸之間的b（1,4）鍵。 它表現出比商業chicken lysozyme雞溶菌酶高四倍的活性，並且可用於回收在細菌（例如大腸桿菌）中表達的蛋白質。 EZ溶菌酶專為溫和的細胞裂解而設計，可使蛋白質變性的風險降至最低。
| Antibody Target | LYZ |
| Alternate Name | 1,4-beta-N-acetylmuramidase C, LYZ, LZM |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Human Lysozyme (Cat. No. 8005) |
| Accession # | P61626 |
| Gene ID | 4069 |
| Appearance | Colorless liquid |
| Concentration | 0.5 mg/ml |
| Formulation | In PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol |
| Purification | Protein A purified |
| Species Reactivity | Human |
| Application | WB |
| Positive Control | WB: human lysozyme |
| Application & Usage | WB: 1-4 µg |