【BDNF檢測】血清腦源性神經營養因素ELISA檢測、多株抗體、BDNF重組蛋白Abcam (Biovision)
【BDNF人腦源性神經營養因子】ELISA檢測、多株抗體、BDNF重組蛋白-Abcam (Biovision)
腦源性神經營養因子/血清腦源性神經營養因素，也稱為（Human Brain-Derived Neurotrophic Factor , BDGF），是一種有效的神經營養因子 (neurotrophic factor)，為生長因子的神經營養因子家族 (neurotrophin family) 的成員，其與經典神經生長因子 ( canonical Nerve Growth Factor) 有關。 BDNF作用於中樞神經系統 (central nervous system ) 和周圍神經系統 (peripheral nervous system) 的某些神經元 (certain neurons)，有助於支持現有神經元的存活 ( survival )，並促進新神經元和突觸的生長(growth )和分化(differentiation)。此外，神經營養型酪氨酸激酶受體2型（Neurotrophic Tyrosine Kinase Receptor Type 2, NTRK2）已被鑑定為BDNF的相互作用因子，因此進行結合ELISA測定以檢測重組人BDNF與重組人NTRK2的相互作用。 BDNF蛋白是27.0kDa二聚體，由兩個相同的119個氨基酸亞基通過強疏水相互作用結合在一起形成。BDNF系列 產品: BDNF (human) ELISA Kit 檢測人類血清(Serum) 、細胞培養上清液 (Cell culture supernatants) 、尿液(Urine)等不同來源之體內BDNF含量。提供多株BDNF腦源性神經營養因子抗體 可應用於Westren Blot實驗，並以BDNF腦源性神經營養因子重組蛋白 作為Positive control對照組。
BDNF (human) ELISA Kit檢測試劑盒 (酶聯免疫吸附試驗法) |貨號K4788-100
產品介紹: BioVision的人BDNF ELISA（酶聯免疫吸附測定）試劑盒是用於定量測定人BDNF的體外酶聯免疫吸附測定。該測定使用在96孔板上包被的人BDNF特異性抗體。將標準品和樣品吸移到孔中，並將樣品中存在的BDNF通過固定的抗體結合到孔上。洗滌孔並加入生物素化的抗人BDNF抗體。洗去未結合的生物素化抗體後，將HRP結合的鏈黴親和素移液至孔中。再次洗滌孔，向孔中加入TM B底物溶液，並且與BDNF結合的量成比例地顯色。停止溶液將顏色從藍色改變為黃色，並且在450nm測量顏色的強度。該ELISA試劑盒不與任何測試的細胞因子反應（例如人血管生成素，BDNF，BLC，ENA-78，FGF-4，IL-1α，IL-1β，IL-2，IL-3，IL- 4，IL-5，IL-7，IL-8，IL-9，IL-10，IL-11，IL-12p70，IL-12p40，IL-13，IL-15，IL- 10，G-CSF，GM-CSF，IFN-γ，瘦蛋白，MCP-1，MCP-2，MCP-3，MDC，MIP-1α，MIP-1δ，PARC，PDGF，RANTES，SCF，TARC， β，TIMP-1，TIMP-2，TNF-α，TNF-β，TPO，VEGF）。 BDNF的最小可檢測劑量通常小於80pg / ml。檢測範圍：80pg / ml-16ng / ml。測定內重現性為CV <10％，測定間CV為12％。
| Size | 100 assays |
| SKU+Size | K4788-100 |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | human |
| Applications | Quantitative protein detection, establishing normal range, validation of antibody array results |
| Features & Benefits | • Easy, convenient and time-saving method to assay BDNF. • The minimum detectable concentration of BDNF is less than 80 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF). |
腦源性神經營養因子（BDNF）酶聯免疫吸附測定試劑盒 | 貨號SEA011Hu
ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)
商品: 腦源性神經營養因子（BDNF）酶聯免疫吸附測定試劑盒 | 貨號SEA011Hu，設計方式為Double-antibody Sandwich。偵測的靈敏度31.2-2,000pg/mL、最低靈敏度12.1pg/mL。應用物種Homo sapiens (Human)； 可應用於serum, plasma, tissue homogenates, cell lysates, cell culture supernates生物樣品之檢測。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
腦源性神經營養因子（BDNF）檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
A potent neurotrophic factor supporting the growth and survival of nerve/glial cells
產品描述：人腦源性神經營養因子/血清腦源性神經營養因素（Human Brain-Derived Neurotrophic Factor , BDGF）是一種有效的神經營養因子 (neurotrophic factor)，可支持神經 (nerve) 和神經膠質細胞 (nerve and glial cells) 的生長和存活。 BDNF蛋白是27.0kDa二聚體，由兩個相同的119個氨基酸亞基通過強疏水相互作用結合在一起形成。 抗體識別14kDa的成熟形式的BDNF。
| Cat # +Size | 5004-100 |
| Size | 100 µg |
| Antibody Target | BDNF |
| Alternate Name | brain-derived neurotrophic factor, brain derived neurotrophic factor |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Recombinant human BDNF |
| Accession # | P23560 |
| Gene ID | 627 |
| Appearance | Colorless liquid |
| Concentration | 0.5 mg/ml |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-human BDNF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
| Purification | Affinity purified |
| Species Reactivity | Human |
| Application | Western blot |
| Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. Recombinant human BDNF (Cat.# 4004-10) can be used as a positive control. |
| Handling | The antibody solution should be gently mixed before use. |
產品附贈 Positive control | Loading抗體 | SABC Kit (三擇一)
BDNF多株抗體 | 貨號PAA011Mi01 –Mutiple
Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)
產品介紹: BDNF多株抗體，可應用於Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse)等物種。此抗體免疫原為RPA011Mi01-Recombinant Brain Derived Neurotrophic Factor (BDNF)； 抗體經由Antigen-specific affinity chromatography純化，並保存於PBS, pH7.4, containing 0.02% NaN3, 50% glycerol。濃度為200ug/ml，最佳實驗濃度請參考操作手冊。抗體之保存: 避免反覆冷凍解凍；4°C保存；若需長期保存請貯存於-20°C。可應用於WB; IHC; ICC; IP.實驗。
Figure. DAB staining on IHC-P; Samples: Mouse Ovary Tissue.
Figure. Western Blot; Sample: Recombinant BDNF
BDNF腦源性神經營養因子重組蛋白 | 貨號4004
A potent neurotrophic factor supporting the growth and survival of nerve/glial cells
產品描述：人腦源性神經營養因子/血清腦源性神經營養因素（Human Brain-Derived Neurotrophic Factor , BDGF）是一種有效的神經營養因子 (neurotrophic factor)，可支持神經 (nerve) 和神經膠質細胞 (nerve and glial cells) 的生長和存活。 BDNF的活性形式是27.0kDa二聚體，由兩個相同的119個氨基酸亞基通過強疏水相互作用結合在一起形成。
| Alternate Name | Brain-derived neurotrophic factor, Short name=BDNF, Abrineurin |
| Gene Symbol | BDNF |
| Gene ID | 627 |
| Accession # | P23560 |
| Source | E. coli |
| Appearance | Lyophilized protein |
| Physical Form Description | Sterile filtered and lyophilized with no additives |
| Molecular Weight | 27.0 kDa |
| Purity by SDS-PAGE | ≥98% |
| Purity by | ≥98% |
| Endotoxin Level | <0.1 ng/μg |
| Biological Activity | The ED₅₀ determined by its ability to stimulate chick E8 neurite outgrowth was found to be 0.1-1.0 ng/ml. |
| Reconstitution Instructions | Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
Active Brain Derived Neurotrophic Factor (BDNF)活性重組蛋白 |貨號APA011Hu61
產品介紹: Cloud clone提供原核及真核生物表現之重組蛋白系列商品；其中Active Glial Cell Line Derived Neurotrophic Factor (GDNF)活性重組蛋白，為活性蛋白。純度> 90%，提供凍乾型式包裝。可應用於Cell culture; Activity Assays.等不同研究。蛋白質保存型式: 蛋白質溶於20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300 為冷凍乾燥包裝。使用前Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL避免vortex. 短期保存Store at 2-8°C for one month.分裝-80°C長期12 個月。請避免反覆冷凍解凍。
將BDNF在含有0.01％BSA（pH7.4）的PBS中連續稀釋。然後將100μL的重複樣品轉移到塗有NTRK2的微量滴定孔中，並在37℃下孵育2小時。用PBST洗滌孔並與抗BDNF pAb一起溫育1小時，然後吸出並洗滌3次。與HRP標記的第二抗體溫育後，吸出孔並洗滌3次。加入底物溶液，將孔在37℃下孵育15-25分鐘。最後，向孔中加入50μL終止溶液，立即讀取450nm。 BDNF和NTRK2的結合活性顯示在圖1中，並且該作用是劑量依賴性的。
(BDNF)活性重組蛋白相關之抗體 | 蛋白質 產品