【報導基因 Reporter cell lines】報導基因穩定細胞株系列商品-ABM代理
報導基因穩定細胞株商品Reporter cell lines
Reporter gene報導基因研究,將細菌,細胞培養物,動物或植物中另一種感興趣的基因調節序列架構於在表現載體上。Abmgood提供系列報導基因螢光素酶,GFP,RFP,雙重報告基因和UTR,穩定表現等細胞株。螢光素酶通常用作報告基因,以評估感興趣的啟動子下螢光素酶基因的遺傳構建體之轉錄活性。螢光素酶穩定的細胞可用於動物模型中的非侵入性可視化,應用於生物體的特徵鑑定和測量。

螢光素酶細胞 tCD-Luc2細胞 | PC3-Luc2細胞 | WM-266-4 -luc2細胞 | AZ-AHR HepG2細胞 | AIZ-AR穩定22Rv1-Luc2細胞 | AZ-GR穩定Hela-luc2 | BRITER-Luc2
AZ-GR穩定HeLa螢光素酶報導細胞 | 貨號T3013
糖皮質激素受體(glucocorticoid receptpr ,GR)是ligand-activated配體活化的轉錄因子,其結合通常用作抗炎或免疫抑制藥物的內源性糖皮質激素,例如cortisol皮質醇和合成的glucocorticoids糖皮質激素。 AZ-GR穩定的HeLa螢光素酶報告細胞, 是檢測GR活性的高通量基因報告物測定,靈敏和可靠的細胞工具。通過將含有與螢光素酶報告基因偶聯的糖皮質激素應答元件(GREs)的質粒穩定轉染到具有內源性GR的HeLa細胞中產生的。這種細胞係對研究新型糖皮質激素藥物或環境應用的臨床前研究特別有效,例如監測soil土壤或water samples水樣中存在的steroid hormone類固醇激素的水平。.
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Cervix |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
HeLa過表達穩定之tCD-Luc2細胞 | 貨號T6023
HeLa過表達穩定性tCD-Luc2細胞, 含有luciferase 2 protein螢光素酶2蛋白和大鼠CD2蛋白之tCD2-luc2融合基因。 通過使用含有融合基因的質粒DNA進行慢病毒感染的穩定轉染,對HeLa細胞中tCD2-luc2的過表達進行。 該細胞系的生長與野生型細胞相同,這表明外源蛋白tCD2-luc2沒有毒性作用。 HeLa過表達穩定的tCD-Luc2細胞系顯示出大量的產生生物發光細胞的螢光素酶活性。 這對於癌症調查具有重要意義的實驗工具是有用的,特別是異種移植動物腫瘤的成像,避免GFP方式作為腫瘤細胞的標記。

HeLa Overexpressing Stable tCD-Luc2 Cell Line
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Cervix |
| Growth Properties | Adherent |
| Morphology | Polygonal |

AIZ-AR穩定22Rv1螢光素酶報導細胞 | 貨號T3014
雄激素受體(The androgen receptor , AR)是參與developmental發育和psychological processes心理過程的ligand-activated配體激活之轉錄因子,並涉及prostatic hyperplasia前列腺增生,prostatic hyperplasia, prostate cancer前列腺癌和改變的pubertal development青春期發育的發病機制。 AIZ-AR穩定的22Rv1螢光素酶報導細胞系含有具有三個拷貝的androgen response region雄激素應答區的報導質粒,其具有下游雄激素應答元件,其允許通過在治療後8小時誘導熒光素酶活性來檢測AR配體。 這些永生化細胞在冷凍保存後功能超過25代,使得它們在研究新藥物或研究可能通過AR相互作用破壞內分泌功能的環境污染物的研究中成為檢測AR配體之可重複和敏感之細胞工具。
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Prostate |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
Luciferase Stable WM-266-4 Cell Line | 貨號T3013
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Skin |
| Growth Properties | Adherent |
| Morphology | epithelial |
| Procedure Overview | |
| Recommended Seeding Density | A subcultivation ratio of 1:3 to 1:6 is recommended |
| Applications | For Research Use Only |
| Donor Age | 58 years |
| Donor Gender | Female |
芳基受體穩定HepG2 Luciferase 報導細胞| 貨號T3012
芳基受體(Aryl hydrocarbon receptor , AhR)是調節與drug metabolism藥物代謝,cell proliferation細胞增殖,immune responses免疫反應,circadian rhythm晝夜節律和tumor promotion腫瘤促進有關的ligand-activated之轉錄因子。 由於AhR激活可能對細胞有許多不同的影響,研究研究新的AhR ligands配體對於藥物相互作用和化學誘導的致癌作用是重要的。 AZ-AHR穩定的HepG2螢光素酶報告基因細胞,係是通過劑量和時間依賴性誘導反應於各種AhR配體螢光素酶活性之複雜與高度敏感的工具。 This immortalized cell line also remains fully functional for over 15 passages increasing efficiency and consistency between experiments.
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Liver |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
Luciferase Stable PC3 Cell Line | 貨號T3006
| BioSafety Level | II |
| Organism | Homo sapiens |
| SourceOrgan | Adenocarcinoma; Prostate |
| Growth Properties | Adherent |
| Morphology | Epithelial |
| Description | Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable PC3 Cells is a luciferase expressing cell line derived from PC3 cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.1ug/ml)-resistant. Luciferase stable PC3 cells can be a very useful cell line for non-invasive visualization in in vitro experiments. |
| Reporter cell lines產品系列 | 細胞株貨號 | 細胞株物種 | 包裝 |
| Luciferase (Luc2) Stable TRAMP Cell Line | T3008 | Mus musculus | 1×106 cells/1.0 ml |
| Luciferase Stable U87MG/ΔEGFR Cell Line | T3009 | Homo sapiens | 1×106 cells/1.0 ml |
| Luciferase Stable PC3 Cell Line | T3006 | Homo sapiens | 1×106 cells/1.0 ml |
| Luciferase Stable WM-266-4 Cell Line | T3013 | Homo sapiens | 1×106 cells/1.0 ml |
| BMP Responsive Immortalized Reporter (BRITER) Cell Line | T3105 | Bmp2c/c;Bmp4c/c; R26CreER Neonatal Mouse | 1×106 cells/1.0ml |
| AZ-AHR Stable HepG2 Luciferase Reporter Cell Line | T3102 | Homo sapiens | 1×106 cells/1.0ml |
| AZ-GR Stable HeLa Luciferase Reporter Cell Line | T3103 | Homo sapiens | 1×106 cells/1.0ml |
| AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line | T3104 | Homo sapiens | 1×106 cells/1.0ml |
| HeLa Overexpressing Stable tCD-Luc2 Cell Line | T6023 | Homo sapiens | 1×106 cells/1.0ml |


