GSH穀胱甘肽染色檢測 | Glutathione Detection Kit (Fluorometric) 貨號K504

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EZCell™ Glutathione Detection Kit (Blue Fluorescence) 穀胱甘肽染色檢測試劑盒(藍色螢光) | 貨號K504
Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells
產品介紹: 穀胱甘肽(GSH)代表活細胞中主要的低分子量游離硫醇。作為哺乳動物細胞中的關鍵抗氧化劑,細胞內GSH形成快速消除與親電異種生物,自由基以及氫過氧化物的結合物。細胞GSH的不足導致氧化應激損傷和線粒體變性。因此穀胱甘肽水平下降是細胞應激的症狀,監測細胞內GSH的分佈作為早期凋亡和細胞死亡的指標是有利的。 BioVision公司的EZCell™穀胱甘肽檢測試劑盒(Blue Fluorescence)使用了一種非螢光和細胞可滲透染料的單氯代苯胺(MCB),它可以在EX / EM = 380/465±20nm處可檢測到GSH(GSH-MCB)形成高度螢光的加合物分別。該反應由穀胱甘肽-S-轉移酶(GST)催化,螢光強度反映細胞內GSH的含量。因此,我們的試劑盒提供了一個有用的工具,用於快速,簡便地評估直接在活細胞中的GSH效應物。

| Size | 100 assays |
| SKU+Size | K504-100 |
| Detection Method | Fluorescence (Ex/Em = 380/460 nm) |
| Applications | • Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells • Screening for compounds affecting intracellular GSH levels |
| Features & Benefits | • Highly sensitive fluorometric method to measure intra-cellular Glutathione levels in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Glutathione metabolism |
| Kit Components | • Wash Buffer • Monochlorobimane (MCB) |
