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觸酶Catalase活性分析套組 | 貨號K773-100 (ABCAM ab83464)

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觸酶Catalase活性分析套組操作手冊

特點

Catalase Activity Colorimetric/Fluorometric Assay Kit觸酶活性分析套組 貨號K773-100 (ABCAM ab83464)

過氧化氫酶(EC 1.11.1.6)是一種普遍存在的抗氧化酶,幾乎存在於所有生物體中。 它的作用是催化過氧化氫(H 2 O 2)分解成水和氧氣。 BioVision的過氧化氫酶分析試劑盒提供高靈敏度,簡單,直接和HTS就緒的分析,用於測量生物樣品中的過氧化氫酶活性。 在測定中,過氧化氫酶首先與H 2 O 2反應生成水和氧,未轉化的H 2 O 2與OxiRed™探針反應生成產物,可在570 nm(比色法)或Ex / Em = 535 / 587nm(螢光測定法)測量。 過氧化氫酶活性與信號成反比。 該試劑盒可檢測樣品中高微量單位的過氧化氫酶。Biovision提供一系列氧化相關分析套組;Catalase活性分析套組,可用於細胞組織supernatants、尿液、血漿、食物、或血清之液體型式的樣品來源。操作時間大約30-40分鐘。最低靈敏度 1 µU of catalase activity。

Catalase Activity Colorimetric/Fluorometric Assay Kit | BioVision, Inc.

功能研究 – 過氧化氫酶檢測試劑盒 (ab83464) 使用 ab83464 進行樣品測試。

Catalase活性分析套組 | K773-100內容物
• Catalase Assay Buffer
• OxiRed™ Probe (in DMSO)
• HRP (lyophilized)
• H₂O₂ (3%; 0.88M)
• Stop Solution
• Catalase Positive Control (lyophilized)

同質商品7

 Catalog #  Product Name+  Size
 K671-100  Ascorbic Acid Colorimetric Assay Kit II (FRASC)  100 assays
 K661-100  Ascorbic Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K773-100  Catalase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K620-100  Ethanol Colorimetric/Fluorometric Assay Kit  100 assays
 K504-100  EZCell™ Glutathione Detection Kit (Blue Fluorescence)   100 assays
 K207-100  EZCell™ Intracellular Nitric Oxide Synthase (NOS) Detection Kit  100 assays
 K264-100  Glutathione (GSH/GSSG/Total) Fluorometric Assay Kit  100 assays
 K261-100  Glutathione Colorimetric Assay Kit  100 assays
 K251-100  Glutathione Fluorometric Assay Kit  100 assays
 K762-100  Glutathione Peroxidase Activity Colorimetric Assay Kit  100 assays
 K761-200  Glutathione Reductase Activity Colorimetric Assay Kit  200 assays
 K263-100  GST Colorimetric Activity Assay Kit  100 assays
 K260-100  GST Fluorometric Activity Assay Kit  100 assays
 K336-100  HAT (H4) Activity Fluorometric Assay Kit  100 assays
 K334-100  HAT Activity Fluorometric Assay Kit  100 assays
 K332-100  HAT Activity Colorimetric Assay Kit  100 assays
 K331-100  HDAC Activity Colorimetric Assay Kit  100 assays
 K330-100  HDAC Activity Fluorometric Assay Kit  100 assays
 K340-100  HDAC Inhibitor Drug Screening Kit (Fluorometric)  100 assays
 K265-200  Hydrogen Peroxide Assay Kit  200 assays
 K712-100  Inosine Fluorometric Assay Kit  100 assays
 K339-100  InSitu HDAC Activity Fluorometric Assay Kit  100 assays
 K204-200  Intracellular Hydrogen Peroxide Detection Kit (cell-based)  200 assays
 K739-100  Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit  100 assays
 K744-100  Myeloperoxidase (MPO) Colorimetric Activity Assay Kit  100 assays
 K745-100  Myeloperoxidase (MPO) Fluorometric Activity Assay Kit  100 assays
 K746-200  Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)  200 assays
 K747-100  Myeloperoxidase (MPO) Peroxidation Activity Assay Kit  100 assays
 K262-200  Nitric Oxide Colorimetric Assay Kit  200 assays
 K252-200  Nitric Oxide Fluorometric Assay Kit  200 assays
 K205-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)  100 assays
 K206-100  Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric)  100 assays
 K208-100  Nitric Oxide Synthase (NOS) Inhibitor Screening Kit (Fluorometric)  100 assays
 K4926-100  NQO1 (human intracellular) ELISA Kit  100 assays
 K772-100  Peroxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays
 K840-100  Protein Disulfide Isomerases (PDI) Inhibitor Screening Kit (Fluorometric)  100 assays
 K335-100  Superoxide Dismutase (SOD) Activity Assay Kit  100 assays
 K763-100  Thioredoxin Reductase Activity Colorimetric Assay Kit  100 assays
 K274-100  Total Antioxidant Capacity (TAC) Colorimetric Assay Kit  100 assays
 K608-100  Uric Acid Colorimetric/Fluorometric Assay Kit  100 assays
 K710-100  Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit  100 assays

相關產品 碳水化合物和脂質代謝 | 胺基酸與無機離子代謝 | 糖酵解途徑和中介代謝 |氧化傷害與血清成分分析

Catalase Activity Assay Kit

  1. Baysal, M et al., (2017) Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress, PLoS One, 2017, 12(4): e0175990
  2. Changhe Zhang, et al., (2018) Blocking of the EGFR-STAT3 signaling pathway through afatinib treatment inhibited the intrahepatic cholangiocarcinoma, Experimental and Therapeutic Medicine, Apr. 2018, 29805522
  3. Emanuela Branchetti et al., Antioxidant Enzymes Reduce DNA Damage and Early Activation of Valvular Interstitial Cells in Aortic Valve Sclerosis. Arterioscler Thromb Vasc Biol, Feb 2013; 33: e66 – e74.
  4. Feng, Chunsheng et al. (2016) Lycopene protects human SH‑SY5Y neuroblastoma cells against hydrogen peroxide‑induced death via inhibition of oxidative stress and mitochondria‑associated apoptotic pathways, Mol Med Rep. 2016 May;13(5):4205-14.
  5. Héritier, L. et al., (2017) Oxidative stress induced by glyphosate-based herbicide on freshwater turtles, Environmental Toxicology and Chemistry, Aug.2017
  6. Liu, Tao et al. (2017) Lutein protects against β-amyloid peptide-induced oxidative stress in cerebrovascular endothelial cells through modulation of Nrf-2 and NF-κb, Cell Biol Toxicol. 2017 Feb;33(1):57-67.
  7. Marni E. Cueno, et al., (2018) Gingival Periodontal Disease (PD) Level-Butyric Acid Affects the Systemic Blood and Brain Organ: Insights Into the Systemic Inflammation of Periodontal Disease, Frontiers in Microbiology, Jun. 2018, 29915575
  8. Mollica, Adriano et al. (2017) An assessment of the nutraceutical potential of Juglans regia L. leaf powder in diabetic rats, Food Chem Toxicol. 2017 Mar 30.
  9. Mostafa Ibrahim Waly, The Protective Effect of Curcumin against Nitrosamine-Induced Gastric Oxidative Stress in Rats. Prev Nutr Food Sci, Dec 2018; 30675457.
  10. Parrillo, Lucia et al. (2017) Olive mill wastewater-enriched diet positively affects growth, oxidative and immune status and intestinal microbiota in the crayfish, Astacus leptodactylus, Aquaculture. 2017 Apr;473: 161-168
  11. Priscila Ramos-Mozo et al., Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression, Arterioscler Thromb Vasc Biol, Dec 2011; 31: 3011 – 3019.
  12. Stephen A. Martin, et al., (2018) GSK3β Regulates Brain Energy Metabolism, Cell Reports, May 2018, 29768193
  13. Xiao et al., Rap1 Ameliorates Renal Tubular Injury in Diabetic Nephropathy. Diabetes, Apr 2014; 63: 1366 – 1380.
  14. Zhigang Hong et al., Role of Dynamin-Related Protein 1 (Drp1)-Mediated Mitochondrial Fission in Oxygen Sensing and Constriction of the Ductus Arteriosus. Circ. Res., Mar 2013; 112: 802 – 815.

 

 

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