Sirtuin 活性檢測試劑盒 | Sirtuin Activity Assay Kit Abcam 貨號(ab287850)
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Sirtuin 活性檢測試劑盒 | SIRT1 活性檢測試劑盒 | NMN | NAD+/NADH 比色測定試劑盒 | 細胞衰老檢測試劑盒 | Metformin | 雷帕黴菌（Rapamycin）
Sirtuin 活性檢測試劑盒 | Abcam 貨號(ab287850)
Sirtuin Activity Assay Kit (ab287850)
使用 ab287850 的標準曲線範例。每次檢測都需要一條新的標準曲線。
ab287850 測量大鼠肝裂解液 (5 µg)、HeLa 細胞裂解液 (160 µg)、HeLa 核提取物 (11.5 µg) 和陽性對照 (2 µl) 中的 Sirtuin 和 Sirtuin + HDAC 活性。按照試劑盒方案進行測定。
ab287850 測量大鼠肝裂解物 (5 µg)、HeLa 細胞裂解物 (160 µg)、HeLa 核提取物 (11.5 µg) 和陽性對照 (2 µl) 中的 Sirtuin 和 Sirtuin + HDAC 比活性。按照試劑盒方案進行測定。
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Product name
Sirtuin Activity Assay Kit -
Detection method
Fluorescent -
Sample type
Cell Lysate, Nuclear Extracts, Tissue Lysate, Purified mitochondria -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
In this Sirtuin Activity Assay Kit (ab287850), the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC’s also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC’s in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 µU of recombinant human SIRT6.
Sirtuin 活性檢測試劑盒 | SIRT1 活性檢測試劑盒 | NMN | NAD+/NADH 比色測定試劑盒 | 細胞衰老檢測試劑盒 | Metformin | 雷帕黴菌（Rapamycin）
