PCR Clean-Up & Gel Extraction Kit | Bio-Helix 貨號PDC01-0100

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PCR Clean-Up & Gel Extraction Kit| Bio-Helix 貨號PDC01-0100

PCR Clean-Up & Gel Extraction Kit

產品描述:

PCR clean up和凝膠提取試劑盒(The PCR Clean-Up & Gel Extraction Kit) 提供了一個經濟高效的系統，用於從 PCR 反應(PCR reactions)、瓊脂糖凝膠(agarose gels)或酶促反應(enzymatic reactions)中快速、輕鬆地分離 DNA 片段。特殊緩衝液中的 DNA 片段（100bp~10Kb）被離心柱的玻璃纖維基質( glass fiber matrix)束縛，而污染物則通過離心柱。雜質被有效洗掉，用 Tris 緩衝液或水洗脫，無需酚抽提或醇沉。使用該試劑盒純化的 DNA 適用於任何後續應用，例如連接和轉化(transformation)、測序(sequencing)、限制酶消化(restriction enzyme digestion)、標記(labeling)、PCR、體外轉錄( in vitro transcription)或顯微注射(microinjection)。整個過程可在15~20分鐘內完成。

Step 1 – Sample Preparation

PCR Clean Up
1. Add 500 μl of the Buffer B to 100 μl of the PCR product and mix by vortex.
Gel Extraction
1. Excise the DNA fragment from the agarose gel.
2. Transfer up to 300 mg of the gel slice to a 1.5 ml microcentrifuge tube.
3. Add 500 μl of the Buffer B to the sample and mix by vortex. Incubate at 60°C for 10 minutes
(or until the gel slice has completely dissolved).
4. During the incubation, mix by vortexing the tube every 2~3 minutes.
5. Cool the dissolved sample mixture to the room temperature.

Step 2 – Binding
1. Place a PG Column in a Collection Tube. Apply the supernatant (from step 1) to the PG Column by decanting or pipetting.
2. Centrifuge at 14,000 x g for 30 seconds.
3. Discard the flow-through and place the PG Column back into the same collection tube.
*The maximum volume of the PG Column reservoir is 800 μl. If the sample mixture is more than 800 μl, repeat the DNA Binding Step.

Step 3 – Wash
1. Add 400 μl of the Buffer W1 into the PG Column.
2. Centrifuge at 14,000 x g for 30 seconds.
3. Discard the flow-through and place the PG Column back into the same collection tube.
4. Add 600 μl of the Buffer W2 (ethanol added) into the PG Column.
5. Centrifuge at 14,000 x g for 30 seconds.
6. Discard the flow-through and place the PG Column back into the same collection tube.
7. Centrifuge at 14,000 x g again for 2 minutes to remove the residual Buffer W2.

Step 4 – Elution
1. To elute the DNA, place the PG Column in a clean 1.5 ml microcentrifuge tube.
2. Add 50-200 μl of the Buffer E or H₂O (pH is between 7.0 and 8.5) to the center of each PG Column, let it stand for 2 minutes, and centrifuge at 14,000 x g for 2 minutes.
＊Check the buffers before use for salt precipitation. Redissolve any precipitate by warming to 37°C.

Fastest Procedure: Completed within 30mins
Sample: up to 100μl of PCR Product or 300 mg of Agarose Gel
Recovery: up to 95%

Buffer B: 60 ml
Buffer W1: 45 ml
Buffer W2: 15 ml (Add 60 ml of the ethanol (96~100%) to the Buffer W2 before use)
Buffer E: 10 ml
PG Columns: 100 pcs
Collection Tubes: 100 pcs

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Bio-Helix成立於2007年，專注於為全球生命科學研究人員提供最好的生物試劑。包括 DNA 標記物、蛋白質標記物、預製蛋白質凝膠、核酸純化、PCR試劑等。與世界各地實驗室的科學家們一起努力，不斷提高質量，開始贏得客戶的信任和評價。品牌遍布四十多個國家，已成為生命科學實驗室值得信賴和歡迎的試劑品牌。Bio-Helix個願景，就是有一天我們的壽命可以延長；所有疾病的早期發現和隨後的適當治療都可以提高生活質量。中國古人認為，良醫治未病，治未病。在 Bio-Helix 中，創建了一個新品牌 LifeDirex 來實現這一願景。 LifeDirex 將專注於為診斷工具提供最好和最具創新性的試劑，以促進盡可能早地發現疾病。Bio-Helix使命是提供最優質的產品並開發新技術，幫助研究人員找到尋求科學成就的解決方案。此外，Bio-Helix認為現在是進入體外診斷設備（IVDD）市場的最佳時機。我們的目標是成為研究實驗室和IVDD的最佳解決方案提供商。

在改善生活質量和實現科學卓越的願望的驅使下，Bio-Helix以最高的誠信標準運營。還將這種精神延伸到我們的服務和全球價值中，以提供給世界各地的客戶。多樣化的員工隊伍和協作網絡形成了公司文化的完美結合。 Bio-Helix 不僅是一家機會均等的公司，而且還努力提供友好和成長的環境。

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