ABBKINE細胞染劑 | CFDA/DAPI細胞染色-Abbkine染劑
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螢光染料 (Fluorescent Dyes)-5(6)-CFDA, SE
5(6)-CFDA, SE | 5-(and-6)-Carboxyfluorescein diacetate 貨號BMD0066
細胞染色(Cell staining)是追踪複雜環境中細胞的重要工具，廣泛應用於細胞遷移(cell migration)、傷口癒合(wound healing)和幹細胞分化研究(stem cell differentiation studies)。細胞運動(Cell movement)和定位研究(localization studies )需要特殊的探針染料，必須對活細胞無毒(non-toxic )，同時有多種螢光顏色可供選擇，以匹配不同的儀器激光波長和濾光片，可以標記抗體或其他細胞分析標記探針共染。Abbkine提供耐光性強、毒性低的螢光染料，如：鬼筆環肽(phalloidin)、Cy5、Cy3染料、Biotin SE、DAPI等。
5(6)-CFDA, SE（全名：5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester，也稱為 CFSE）是一種有用的螢光示踪劑(fluorescent tracer)，可以被動擴散到細胞中並共價標記細胞內蛋白質(intracellular proteins)，從而導致長期細胞標記。該試劑本身是無色無螢光的，但一旦被細胞內酯酶(intracellular esterases)水解，就會變成亮綠色螢光。
| Product name | 5(6)-CFDA, SE |
| CAS Number | 150347-59-4 |
| Molecular Formula | C29H19NO11 |
| Alternative | 5(6)-CFDA, SE,5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester; CFSE; BMD00066 |
Product Properties
| Formulation | White solid or Pale yellow solid |
| Features & Benefits | • λEX/λEm: 494/521。（pH=7） |
| Molecular weight | 557.47 |
| Usage notes | • Soluble in DMSO |
| Storage instructions | Store at -20°C and protect from light. Product is stable for at least 12 months from date of receipt if stored as recommended. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
DAPI Staining Solution 粉狀 (DNA staining) | 貨號BMD00063
產品描述： DAPI是一種流行的藍色螢光DNA染料(blue fluorescent DNA dye)。該染料與dsDNA的結合，螢光增強約20倍。由於DAPI穿過完整的細胞膜，因此可用於染色活細胞和固定細胞。DAPI通常用於細胞凋亡檢測，染色後將使用螢光顯微鏡(fluorescence microscope)或流式細胞儀(flow cytometer)進行檢測。DAPI的藍色發射便於進行多重分析，因為DAPI與綠色螢光分子（如FITC和綠色螢光蛋白（GFP）或紅色螢光染料（如Texas Red）之間幾乎沒有螢光重疊。
Fig. DAPI structure formula
| Product name | DAPI |
| CAS Number | 28718-90-3 |
| Molecular Formula | C16H17Cl2N5 |
| Alternative | DAPI,BMD00063 |
| Formulation | Yellow powder |
| Features & Benefits | • λEx/λEm: 360/460 nm (with DNA) |
| Molecular weight | 350.25 |
| Usage notes | • Soluble in H2O |
| Storage instructions | Store at -20°C and protect from light. Product is stable for at least 12 months from date of receipt if stored as recommended. |
| Shipping | Gel pack with blue ice. |
| Background | DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement. DAPI is often applied to apoptosis detection, which will use fluorescence microscope or flow cytometer after staining. |
| Alternative | DAPI,BMD00063 |
引用文獻
Ruilian Li, Xianghua Yuan, Jinhua Wei, et al. Mar Drugs. 2019 Jan 10;17(1). pii: E43.
AbFluor™ 488-Phalloidin (Ex/Em = 490/515 nm)
F-actin肌動蛋白染色套組 (AbFluor™ 488-Phalloidin) | 貨號BMD00082
F-actin肌動蛋白是一種大約42 kDa的蛋白質，幾乎在所有真核細胞中都有發現，它也是最高度保守的蛋白質之一。肌動蛋白的單體為球形分子，稱為球形肌動蛋白G-actin，它的多聚體稱為纖維形肌動蛋白F-actin。這兩者對於細胞分裂過程中細胞的移動和收縮等重要的細胞功能都是必不可少的。AbFluor™ 488-Phalloidin選擇性地結合F-actins, 它比氟西林-鄰苯二甲a)的抗性要高得多。Phalloidin衍生物 是標記、識別和定量甲醛固定(formaldehyde-fixed )和滲透組織部分( permeabilized tissue sections)中的F-actin的方便探針,可在細胞培養物中染色F-actin或cell-free experiments at nanomolar levels。
鄰苯二甲苯甲類毒素(Phalloidin )
Phalloidin 為一種帶有毒性的胜肽，具有與 F-actin 專一性結合的特性。屬於一類稱為phallo毒素(phallotoxins)的毒素,這些毒素與死亡帽蘑菇(阿馬尼塔phalloides)分離。它是一種二環肽(bicyclic peptide ),特別與F-actin結合。因此,使用螢光染料標記的鄰苯二甲二素素,可以非常方便地研究F-actin的分佈。在噬菌素內部,半胱氨酸和色氨酸之間有一個不尋常的硫醚橋,可以形成一個內環結構。當pH升高時,硫醚被切合,鄰苯二甲酸酯失去對作用素的親和力。標定有螢光染料的 Phalloidin 試劑產品，能夠協助研究人員精確掌握細胞內 F-actin 的分布情形、了解 F-actin 表現量變化。
產品介紹: AbFluor™ F-actin 488-Phalloidin染色試劑盒(Gree Fluorescence, 綠色螢光)使用綠色螢光(Ex/Em = 490/515 nm)鄰苯二甲素偶聯,選擇性地結合F-actins,其光穩定性遠遠高於其他氟素-噬菌素偶聯(fluorescein-phalloidin conjugates)。Phalloidin衍生物(fluorescein-phalloidin conjugates.)是標記、識別和定量甲醛固定和滲透組織部分中的F-actin探針,可在細胞培養物中染色F-actin或cell-free experiments at nanomolar levels。
- FormulationLyophilized powder
- Features & Benefits
- Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
- Proprietary AbFluor™ 488-Phalloidin (Green Fluorescence) (Ex/Em = 490/515 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
- Usage notesPhalloidin is toxic, it should be handled with care.
- Storage instructionsStable for at least 12 months if store at -20°C. Protect this product from light.
- ShippingGel pack with blue ice.
| Product Name | Catalog No |
| TraKine™ Cell Plasma Membrane Staining Kit (Green Fluorescence) | KTC4001 |
| TraKine™ Cell Plasma Membrane Staining Kit (Orange Fluorescence) | KTC4002 |
| TraKine™ Mitochondrion Staining Kit (Green Fluorescence) | KTC4003 |
| TraKine™ Mitochondrion Staining Kit (Orange Fluorescence) | KTC4004 |
| TraKine™ Mitochondrion and Nuclear Staining Kit | KTC4005 |
| TraKine™ F-actin Staining Kit (Green Fluorescence) | KTC4008 |
| TraKine™ F-actin Staining Kit (Orange Fluorescence) | KTC4009 |
| TraKine™ Pro Live-cell Tubulin-traker kit (Green Fluorescence) | KTC4100 |
| TraKine™ Pro Live-cell Lyso-traker kit (Red Fluorescence) | KTC4210 |
| TraKine™ Pro Live-cell Tubulin-traker kit (Red Fluorescence) | KTC4110 |
| TraKine™ Pro Live-cell Lyso-traker kit (Green Fluorescence) | KTC4200 |
| Cy3 | BMD0050 |
| Cy5 | BMD0051 |
| Ethidium Homodimer-1 (EthD-1) | BMD0060 |
| Hoechst 33258 | BMD0061 |
| Hoechst 33342 | BMD0062 |
| DAPI | BMD0063 |
| Calcein AM | BMD0064 |
| 5(6)-CFDA, SE | BMD0066 |
| DiI (DiIC18(3)) | BMD0071 |
| DiO (DiOC18(3)) | BMD0072 |
| DiD (DiIC18(5)) | BMD0073 |
| DiR (DiIC18(7)) | BMD0074 |
| AbFluor™ 488-Phalloidin | BMD0082 |
| AbFluor™ 555-Phalloidin | BMD0083 |
| AbFluor™ 594-Phalloidin | BMD0084 |