ExKine™ 粒線體提取試劑盒（細胞） | ExKine™ Mitochondrion Extraction Kit (Cultured Cells)貨號KTP4003

粒線體(Mitochondria)是一種雙膜結合(double-membrane-bound)的細胞器(organelle)，存在於大多數真核細胞(eukaryotic cells)中。粒線體(mitochondria)的功能是提供細胞能量(cellular energy)。此外，粒線體(Mitochondria)還參與其他任務，例如信號傳導(signaling)、細胞分化(cellular differentiation)和細胞死亡(cell death)，以及維持對細胞週期(cell cycle)和細胞生長(cell growth)的控制。粒線體(Mitochondria)與多種人類疾病有關，包括粒線體疾病(mitochondrial disorders)、心臟功能障礙(cardiacdysfunction)、心力衰竭(heart failure)和自閉症(autism)。粒線體(Mitochondria)可能在這些細胞過程中發揮重要作用。Abbkine ExKine™ 粒線體(Mitochondria)提取試劑盒（培養細胞）能夠快速、粗略地從培養的哺乳動物細胞中分離出完整的粒線體(Mitochondria)。該試劑盒提供兩種方案，用於依靠差速離心從細胞溶質成分(cytosolic components relying)中分離粒線體(Mitochondria)。第一個協議採用基於試劑的方法，允許同時處理多個樣本。第二個協議使用傳統的 Dounce 均質化，並提供大約兩倍的粒線體(Mitochondria)。

Fig. 293T cells were extracted with ExKine™ Mitochondrion Extraction Kit (Cultured Cells). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191), VDAC1 antibody (cat#ABP53121) and COX IV antibody (cat#A01060).

Product name	ExKine™ Mitochondrion Extraction Kit (Cultured Cells)
Kit components	• Lysis Buffer A (5×) • Lysis Buffer B • Lysis Buffer C • Storage Buffer
Features & Benefits	• Isolate intact mitochondria from 20 million cells in less than 1 hour. • Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method. • Isolated mitochondria is compatible with almost any downstream application.
Usage notes	Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Storage instructions	Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.
Shipping	Gel pack with blue ice.

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