Hi-Bind™ Protein G-Agarose | 貨號 6513 規格1ml/5ml/25ml/100ml (ABCAM, ab193258)

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相關下載：	Hi-Bind™ Protein G-Agarose操作手冊

特點

Hi-Bind™ Protein G-Agarose | 貨號 6513 規格1ml/5ml(ABCAM, ab193258) /25ml/100ml

Hi-Bind™ 蛋白 G-瓊脂糖珠(通過將重組蛋白 G 共價偶聯至 6% 交聯瓊脂糖珠（蛋白質親和純化方法最常用的樹脂）而專門製備用於高 IgG 結合。 BioVision 的 Protein G（貨號 # 6510）是一種基因工程蛋白，包含天然 Protein G 的三個 IgG 結合區域。細胞壁結合區域、白蛋白結合區域和其他非特異性區域已從重組 Protein G 中去除，以確保最大的特異性 IgG 結合。偶聯技術經過優化，與市場上的其他常規蛋白 G 瓊脂糖相比，具有更高的 IgG 結合能力和最少的重組蛋白 G 的浸出。 Hi-Bind™ Protein G-Agarose 的 IgG 結合能力為每毫升濕珠 ≥ 30 mg 人或兔 IgG。 Hi-Bind™ 蛋白 G-瓊脂糖珠具有穩定的化學和物理特性，具有高流速、親水性和高凝膠強度。

Highlights

• CONTENTS: Supplied as 50% slurry in 20 % Ethanol/H2O.
• FEATURES:
– HIGH BINDING CAPACITY: Binding of IgG >20 mg human or rabbit IgG/ml Protein G-Sepharose.
– MINIMAL LEACHING OF THE LIGAND.
– FLOW RATE TESTED*: 2.07 ml/min.
*Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
– USAGE: Reusable for up to 10 times without significant loss of binding capacity.
• APPLICATIONS
– Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
– Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

Protein A／G／L重組蛋白介紹

Protein A、G和L已被廣泛地用於抗體之純化；BioVision公司提供廣泛的Agarose，Sepharose及Protein A、Protein G、Protein L、ProteinA/G、ProteinA/G/L磁珠商品。其含有IgG結合位，可和大多數免疫球蛋白之Fc或輕鏈區域結合，純化單株或多株抗體。其應用之樣品，可來自培養液culture media、血清serum、腹水ascites fluid或Hybridoma supernatants；亦可分離Immunoprecipitation實驗中抗體與抗原的複合物。

不同物種純化之效能比較

Protein A／G／L重組蛋白產品特點

專一性高：已排除白蛋白、細胞壁、及其他非專一蛋白結合區。
選擇性多：有Protein A、Protein G、Protein L、Protein A/G、Protein A/G/L等商品。依不同實驗需求提供磁珠、Agarose／Sepharose、Sepharose Column等產品
品質佳：SDS-PAGE和HPLC純化，純度＞98％；內毒素＜1EU/mg
具有高效的抗體結合能力
高化學和物理穩定性
展現高流速、親水性和高凝膠強度

Protein A、G：從E. coli純化出僅包含IgG binding domains的蛋白，可和大多數免疫球蛋白之Fc區域（如重鏈）結合。Protein L：包含Kappa light chain binding region，可以和各類Ig（IgG、IgM、IgA、IgE和IgD）的輕鏈結合。除此之外，Single chain variable fragments（ScFv）和 Fab fragments也會和Protein L結合。

1. Adnan Shami Shah, PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling. Cell Rep., May 2019; 31091453.

2. Melina V. Jones, Effect of CXCR2 Inhibition on Behavioral Outcomes and Pathology in Rat Model of Neuromyelitis Optica. J Immunol Res, Dec 2018, 30648122.

3. Wicht et al., Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell. J. Virol., May 2014; 88: 4943 – 4952.

4. Jia et al., Solution Structure, Membrane Interactions, and Protein Binding Partners of the Tetraspanin Sm-TSP-2, a Vaccine Antigen from the Human Blood Fluke Schistosoma mansoni.J. Biol. Chem., Mar 2014; 289: 7151 – 7163.

5. Price et al., CD8+ dendritic cell-mediated tolerance of autoreactive CD4+ T cells is deficient in NOD mice and can be corrected by blocking CD40L. J. Leukoc. Biol., Feb 2014; 95: 325 – 336.

不同型式之beads商品

Catalog #	Product Name+	Size
7934-1	Calmodulin-Sepharose Beads	1 ml
7934-5	Calmodulin-Sepharose Beads	5 ml
7934-10	Calmodulin-Sepharose Beads	10 ml
7933-1	Hi-Bind™ Albumin-IgG Depletion Beads	1 ml
7933-5	Hi-Bind™ Albumin-IgG Depletion Beads	5 ml
7933-25	Hi-Bind™ Albumin-IgG Depletion Beads	25 ml
7931-1	Immobilized Catalase Beads	1 ml
7931-10	Immobilized Catalase Beads	10 ml

Protein A重組蛋白相關商品

Catalog #	Product Name+	Size
6520-1	Hi-Bind™ Protein A-Agarose	1 ml
6520-100	Hi-Bind™ Protein A-Agarose	100 ml
6520-25	Hi-Bind™ Protein A-Agarose	25 ml
6520-5	Hi-Bind™ Protein A-Agarose	5 ml
6520-500	Hi-Bind™ Protein A-Agarose	500 ml
6500-10	Protein A (Liquid form)	10 mg
6500-100	Protein A (Liquid form)	100 mg
6500-1000	Protein A (Liquid form)	1 g
6500-25	Protein A (Liquid form)	25 mg
6500B-10	Protein A (Lyophilized)	10 mg
6500B-100	Protein A (Lyophilized)	100 mg
6500B-1000	Protein A (Lyophilized)	1 g
6500B-25	Protein A (Lyophilized)	25 mg
5500-100	Protein A Antibody	100 µg
6524-1000	Protein A IgG Binding Buffer	1000 ml
6524-3750	Protein A IgG Binding Buffer	3750 ml
K6529-3	Protein A IgG Purification Buffer Kit	3 Buffers
6507-1	Protein A Magnetic Beads	1 ml
6501-1	Protein A Sepharose	1 ml
6501-100	Protein A Sepharose	100 ml
6501-25	Protein A Sepharose	25 ml
6501-5	Protein A Sepharose	5 ml
6508-1	Protein A Sepharose Column	1 ml
6508-5	Protein A Sepharose Column	5 ml
6526-1	Protein A-Agarose	1 ml
6526-5	Protein A-Agarose	5 ml
6526-25	Protein A-Agarose	25 ml
6526-100	Protein A-Agarose	100 ml

Protein G重組蛋白相關商品

Catalog #	Product Name+	Size
6513-1	Hi-Bind™ Protein G-Agarose	1 ml
6513-5	Hi-Bind™ Protein G-Agarose	5 ml
6513-25	Hi-Bind™ Protein G-Agarose	25 ml
6513-100	Hi-Bind™ Protein G-Agarose	100 ml
6510-1	Protein G	1 mg
6510-10	Protein G	10 mg
6510-100	Protein G	100 mg
6510-1000	Protein G	1 g
6510-5	Protein G	5 mg
5510-100	Protein G Antibody	100 µg
6522-5	Protein G Coated 96-well Plates	5 plates
6517-1	Protein G Magnetic Beads	1 ml
6511-1	Protein G Sepharose	1 ml
6511-100	Protein G Sepharose	100 ml
6511-25	Protein G Sepharose	25 ml
6511-5	Protein G Sepharose	5 ml
6518-1	Protein G Sepharose Column	1 ml
6518-5	Protein G Sepharose Column	5 ml
6512-100	Protein G-Biotin	100 µg
6512-1000	Protein G-Biotin	1 mg

Protein L重組蛋白相關商品

Catalog #	Product Name+	Size
6530-1	Protein L	1 mg
6530-10	Protein L	10 mg
6530-100	Protein L	100 mg
6530-5	Protein L	5 mg
5530-100	Protein L Antibody	100 µg
6537-1	Protein L Magnetic Beads	1 ml
6531-1	Protein L Sepharose	1 ml
6531-100	Protein L Sepharose	100 ml
6531-25	Protein L Sepharose	25 ml
6531-5	Protein L Sepharose	5 ml
6538-1	Protein L Sepharose Column	1 ml
6538-5	Protein L Sepharose Column	5 ml

Protein A/G重組蛋白相關商品

Catalog #	Product Name+	Size
6502-1	Protein A/G	1 mg
6502-100	Protein A/G	100 mg
6502-1000	Protein A/G	1 g
6502-25	Protein A/G	25 mg
6502-5	Protein A/G	5 mg
6527-1	Protein A/G Magnetic Beads	1 ml
6503-1	Protein A/G Sepharose	1 ml
6503-100	Protein A/G Sepharose	100 ml
6503-25	Protein A/G Sepharose	25 ml
6503-5	Protein A/G Sepharose	5 ml
6528-1	Protein A/G Sepharose Column	1 ml
6528-5	Protein A/G Sepharose Column	5 ml
6506-01	Protein A/G-Biotin	100 µg
6506-1	Protein A/G-Biotin	1 mg
6504-01	Protein A/G-FITC	100 µg
6504-1	Protein A/G-FITC	1 mg

Protein G/L重組蛋白相關商品

Catalog #	Product Name+	Size
6540-1	Protein A/G/L	1 mg
6540-100	Protein A/G/L	100 mg
6540-1000	Protein A/G/L	1 g
6540-25	Protein A/G/L	25 mg
6540-5	Protein A/G/L	5 mg
6547-1	Protein A/G/L Magnetic Beads	1 ml
6541-1	Protein A/G/L Sepharose	1 ml
6541-100	Protein A/G/L Sepharose	100 ml
6541-25	Protein A/G/L Sepharose	25 ml
6541-5	Protein A/G/L Sepharose	5 ml
6548-1	Protein A/G/L Sepharose Column	1 ml
6548-5	Protein A/G/L Sepharose Column	5 ml

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