Mouse Anti-Biotin-UNLB抗體 | 貨號6404-01 規格0.5 mg (0.5 mg/mL)

Mouse Anti-Biotin-UNLB抗體 | 貨號6404-01 規格0.5 mg (0.5 mg/mL)
美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。
二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白(immunoglobulin , Ig)分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用,並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用,例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記,這使得能夠通過比色,化學發光和螢光技術進行檢測。例如,酶辣根過氧化物酶(enzymes horseradish peroxidase , HRP)和鹼性磷酸酶(alkaline phosphatase , AP)通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素(fluorescein , FITC),藻紅蛋白(phycoerythrin , PE)和花青(cyanine , CY)的螢光染料在螢光的應用中是必不可少的,包括流式細胞術,免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大,可增加的靈敏度。
產品介紹: Mouse Anti-Biotin-UNLB抗體,不具標誌。針對Biotin具特異性, 免疫原為Biotin-KLH。Isotype為Mouse (BALB/c) IgG1κ。Isotype ControlMouse IgG1-UNLB。 緩衝液配方在Borate buffered saline, pH 8.2。
| Clone | SB58c |
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-UNLB |
| Immunogen | Biotin-KLH |
| Specificity | Biotin |
| Description | Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives. |
| Format/Conjugate | UNLB (Unconjugated) |
| Buffer Formulation | Borate buffered saline, pH 8.2 |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Storage & Handling | 2-8°C Please refer to product specific SDS |
| Applications |
Applications for relevant formats of this clone include – ELISA – Quality tested |
| Recommended Dilutions | Please refer to product specific Technical Bulletin |
| RRID | AB_2796296 |
SBA熱銷商品 7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 | DAPI螢光封片劑 | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I | 一級抗體
