Rabbit Anti-Sheep IgG(H+L), Human SP ads-HRP抗體 | 貨號6156-05 規格1.0 mL

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

產品介紹:  Rabbit Anti-Sheep IgG(H+L), Human SP ads-HRP抗體抗體，具HRP標誌。針對sheep IgG輕鏈及重鏈以及輕鏈具特異性。Isotype為Rabbit IgG。緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

ELISA plate was coated with purified sheep IgG. Immunoglobulin was detected with Rabbit Anti-Sheep IgG(H+L), Human SP ads-HRP (SB Cat. No. 6156-05).

Specificity	Reacts with the heavy and light chains of sheep IgG
Source	Pooled antisera from rabbits hyperimmunized with sheep IgG
Cross Adsorption	Human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other sheep immunoglobulins
Purification	Affinity chromatography on sheep IgG covalently linked to agarose
Isotype	Rabbit IgG
Format/Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA FLISA Other referenced applications for relevant formats include – Immunohistochemistry-Paraffin Sections 1 Western Blot 2-4
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796215

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

References

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