Mouse Anti-Feline CD4-UNLB抗體 | 貨號8130-01  規格1.0 mL

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

產品介紹:  Mouse Anti-Feline CD4-UNLB，不具標誌。針對Feline/Lion CD4具特異性。Isotype為Goat IgG。 緩衝液配方在Borate buffered saline, pH 8.2。

 

Clone	3-4F4
Isotype	Mouse IgG1κ
Isotype Control	Mouse IgG1-UNLB
Immunogen	Feline T cells
Specificity	Feline/Lion CD4
Alternalte Name(s)	fCD4
Description	Feline CD4, a member of the immunoglobulin superfamily of cell surface receptors, is a type I transmembrane glycoprotein that is expressed on the helper/inducer subpopulation of peripheral T lymphocytes. It is present on approximately 55% of thymocytes, 15% of splenocytes, 40% of lymph node cells, and 25% of peripheral blood lymphocytes. CD4 functions as an accessory molecule in the recognition of foreign antigens in association with MHC Class II antigens by T cells.
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested 1,4,7-31 Immunohistochemistry-Frozen Sections – Reported in literature 3-5 Immunocytochemistry – Reported in literature 2 Immunoprecipitation – Reported in literature 1,2 Separation – Reported in literature 6 Blocking – Reported in literature 1
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796413

 

Gene ID	493775 (Feline)
Gene Symbol	CD4 (Feline)
Uniprot ID	P79355 (Feline)
Uniprot Name	P79355_FELCA
Gene ID	16019 (Mouse)
Gene Symbol	Ighm (Mouse)
Gene Aliases	AI326478; Igh-6; Igh-M; Igh6; Igm; muH
Uniprot ID	P01872 (Mouse)
Uniprot Name	IGHM_MOUSE

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

References

1. Ackley CD, Hoover EA, Cooper MD. Identification of a CD4 homologue in the cat. Tissue Antigens. 1990;35:92-8. (Immunogen, FC, IP, Block)
2. Shimojima M, Morikawa S, Maeda K, Tohya Y, Miyazawa T, Mikami T. Generation of monoclonal antibodies against a feline CD antigen (CD4) expressed by a recombinant baculovirus. J Vet Med Sci. 1997;59:467-9. (IP, ICC)
3. Rideout BA, Lowenstine LJ, Hutson CA, Moore PF, Pedersen NC. Characterization of morphologic changes and lymphocyte subset distribution in lymph nodes from cats with naturally acquired feline immunodeficiency virus infection. Vet Pathol. 1992;29:391-9. (IHC-FS)
4. Femenia F, Crespeau F, Fontaine JJ, Boucheix C, Parodi AL. Early haematological and pathological abnormalities of pathogen-free cats experimentally infected with feline immunodeficiency virus (FIV). Vet Res. 1994;25:544-54. (IHC-FS, FC)
5. Fox JG, Perkins S, Yan L, Shen Z, Attardo L, Pappo J. Local immune response in Helicobacter pylori-infected cats and identification of H. pylori in saliva, gastric fluid and faeces. Immunology. 1996;88:400-6. (IHC-FS)
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7. Spencer JA, Bouic PD, Espie I, Colly L. A comparison between T-helper (CD4) and T-suppressor (CD8) cell ratios in FIV positive and negative lions Panthera leo and domestic cats Felis catus. S Afr J Wildl Res. 1995;25:111-2. (FC, Lion Reactivity)
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17. de Groot-Mijnes JD, van Dun JM, van der Most RG, de Groot RJ. Natural history of a recurrent feline coronavirus infection and the role of cellular immunity in survival and disease. J Virol. 2005;79:1036-44. (FC)
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20. El Garch H, Richard S, Piras F, Leard T, Poulet H, Andreoni C, et al. Feline leukemia virus (FeLV)-specific IFNγ+ T-cell responses are induced in cats following transdermal vaccination with a recombinant FeLV vaccine. Int J Appl Res Vet Med. 2006;4:100-8. (FC)
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29. Simões RD, Howard KE, Dean GA. In vivo assessment of natural killer cell responses during chronic feline immunodeficiency virus infection. PLoS One. 2012;7(5):e37606. (FC)
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