Mouse Anti-Human CD45-CY5抗體 | 貨號9625-15 規格100Test

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Mouse Anti-Human CD45-CY5抗體 | 貨號9625-15 規格100Test

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

CD45，也稱為白細胞共同抗原（LCA），以四種不同的同工型（180、190、205、220 kDa）存在，它們是通過三個外顯子的可變剪接產生的。 LCA存在於所有造血細胞上，但紅細胞和血小板除外。 CD45表達是T細胞和B細胞抗原受體介導的激活所必需的。

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45-CY5 (SB Cat. No. 9625-15).

產品介紹: Mouse Anti-Human CD45-CY5抗體，具HRP標誌。

Clone	F10-89-4
Isotype	Mouse IgG_2aκ
Isotype Control	Mouse IgG_2a-CY5
Immunogen	Purified T cells from human lymph nodes
Specificity	Human/Horse CD45
Alternalte Name(s)	LCA, T200, Ly5
Description	CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.
Format/Conjugate	CY5 (Cyanine 5)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – Flow Cytometry – Quality tested ^1,5,9-11 Immunohistochemistry-Frozen Sections – Reported in literature ^1-3 Immunocytochemistry – Reported in literature ^4,5 Immunoprecipitation – Reported in literature ^6,7 Western Blot – Reported in literature ^7,8
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2797030

Gene ID	5788 (Human) 100061950 (Horse)
Gene Symbol	PTPRC (Human) PTPRC (Horse)
Gene Aliases	B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200
Uniprot ID	P08575 (Human)
Uniprot Name	PTPRC_HUMAN

References

Dalchau R, Kirkley J, Fabre JW. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980;10:737-44. (Immunogen, FC, IHC-FS)
Mason DY, Cordell JL, Abdulaziz Z, Naiem M, Bordenave G. Preparation of peroxidase: antiperoxidase (PAP) complexes for immunohistological labeling of monoclonal antibodies. J Histochem Cytochem. 1982;30:1114-22. (IHC-FS)
Salter DM, Krajewski AS, Dewar AE. Immunophenotype analysis of malignant histiocytosis of the intestine. J Clin Pathol. 1986;39:8-15. (IHC-FS)
Toscano MA, Bianco GA, Ilarregui JM, Croci DO, Correale J, Hernandez JD, et al. Differential glycosylation of TH1, TH2 and TH-17 effector cells selectively regulates susceptibility to cell death. Nat Immunol. 2007;8:825-34. (ICC)
De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, et al. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry. Cytometry A. 2012;81:312-23. (FC, ICC, Horse Reactivity)
Chen I, Chen H, Demetriou M. Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. J Biol Chem. 2007;282:35361-72. (IP)
Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I. Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004;172:4298-307. (IP, WB)
Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu F, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006;176:778-89. (WB)
Young AJ, Holzgreve W, Dudler L, Schoeberlein A, Surbeck DV. Engraftment of human cord blood-derived stem cells in preimmune ovine fetuses after ultrasound-guided in utero transplantation. Am J Obstet Gynecol. 2003;189:698-701. (FC)
Mitchell P, Lee F, Hall C, Rigopoulos A, Smyth FE, Hekman A, et al. Targeting primary human Ph+ B-cell precursor leukemia-engrafted SCID mice using radiolabeled anti-CD19 monoclonal antibodies. J Nucl Med. 2003;44:1105-12. (FC)
Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, et al. Long-term cultures of bone marrow-derived

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