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(植物) 肌動蛋白抗體(3T3)| Anti-Plant Actin Mouse Monoclonal Antibody (3T3) 貨號A01050

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抗體於WB應用之操作手冊

特點

抗植物肌動蛋白小鼠單克隆抗體(3T3)| Anti-Plant Actin Mouse Monoclonal Antibody (3T3) 貨號A01050

產品描述:肌動蛋白(Actin)是高度保守(conserved protein)的蛋白質,是細胞骨架(cell cytoskeleton)的重要組成部分,在細胞質流(cytoplasmic streaming),細胞形狀確定(cell shape determination),細胞分裂(cell division),細胞器運動(organelle movement)和延伸(extension growth)生長中起重要作用。 優先表達於年輕的和不斷擴大的組織,花器官原基(floral organ primordia),發育中的種子(developing seeds)和新興花序(emerging inflorescence)。 擬南芥(Arabidopsis)有一個複雜的肌動蛋白基因家族(actin gene family),編碼六個蛋白質亞類(protein subclasses)。 其中一類由蛋白質ACT2和ACT8代表,它們是相似的蛋白質,但是在密碼子codons中具有不同的側翼序列(flanking sequences),內含子(introns)和沈默核苷酸位置(silent nucleotide positions)。 ACT8在根(root,),莖(stems),葉(leaves),花(flowers),花粉(pollen)和長角果(siliques)中表達。

引用文獻20+

  1. Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)

S Peng, S Huang, Z Liu. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

  1. Phosphorylation-mediated dynamics of nitrate transceptor NRT1. 1 regulate auxin flux and nitrate signaling in lateral root growth

X Zhang, Y Cui. PLANT PHYSIOLOGY

  1. EMS1 and BRI1 control separate biological processes via extracellular domain diversity and intracellular domain conservation

B Zheng, Q Bai. Nature Communications

  1. Retardation of the Calvin cycle contributes to the reduced CO2 assimilation ability of rice stripe virus-infected N. benthamiana and suppresses viral infection

J Bi, Y Yang, B Chen, J Zhao, Z Chen, B Song. Front. Microbiol., 20 March 2019.

  1. Suppression of auxin signalling promotes rice susceptibility to Rice black streaked dwarf virus infection

H Zhang, X Tan, L Li, Y He, G Hong, J Li. Molecular plant pathology, 2019.

  1. Rice black‐streaked dwarf virus P10 acts as either a synergistic or antagonistic determinant during superinfection with related or unrelated virus

H Zhang, X Tan, Y He, K Xie, L Li. Molecular plant pathology, 2019.

  1. Suppression of auxin signalling promotes rice susceptibility to Rice black streaked dwarf virus infection

Hehong Zhang, Xiaoxiang Tan, Lulu Li, et al. Mol Plant Pathol. 2019 Jun 27.

  1. Mutation of ACX1, a Jasmonic Acid Biosynthetic Enzyme, Leads to Petal Degeneration in Chinese Cabbage (Brassica campestris ssp. pekinensis)

Shenling Peng, Shengnan Huang, Zhiyong Liu and Hui Feng. Int J Mol Sci. 2019 May 10;20(9). pii: E2310.

  1. Downregulation of Nuclear Protein H2B Induces Salicylic Acid Mediated Defense Against PVX Infection in Nicotiana benthamiana

Xue Yang, Yuwen Lu, Xing Zhao, et al. Front Microbiol. 2019 May 8;10:1000.

活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶  細胞增殖EdU Image試劑盒 

 

  • Product name

Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

  • Immunogen

Recombinant Protein

  • Host

Mouse

  • Reactivity

Arabidopsis, Corn, Rape

  • Applications

WB

  • Application notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).

  • Clonality

Monoclonal

  • Isotype

Mouse IgG1

  • Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

  • Formulation

Liquid solution

  • Storage buffer

Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

 

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