Goat Anti-Type III Collagen-UNLB抗體 | 貨號1330-01

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原廠連結：	https://www.southernbiotech.com
相關下載：	Goat Anti-Type III Collagen-UNLB抗體抗體操作手冊

特點

Goat Anti-Type III Collagen-UNLB抗體 | 貨號1330-01 規格200ug (0.4 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質一級抗體及二級抗體，目前除了一般常見的多株與單株抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

產品介紹: 山羊抗III型膠原蛋白-UNLB抗體，不具標誌。針對type III collagen具特異性。來源為Pooled antisera from goats hyperimmunized with type III collagen。Isotype為Goat IgG。Isotype Control Goat IgG-UNLB。緩衝液配方在Borate buffered saline, pH 8.2。

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

Frozen osteopontin-null mouse muscle section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.

Paraplast Plus® embedded 2K1C rat kidney section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody and DAB and hematoxylin.

Image from Cuevas CA, Tapia-Rojas C, Cespedes C, Inestrosa NC, Vio CP. β-catenin-dependent signaling pathway contributes to renal fibrosis in hypertensive rats. Biomed Res Int. 2015;2015:726012. Figure 2(e)

Paraplast Plus® is a registered trademark of Leica Biosystems Richmond, Inc.

Frozen kidney section following unilateral ureteral obstruction was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.

Image from Maeshima A, Mishima K, Yamashita S, Nakasatomi M, Miya M, Sakurai T, et al. Follistatin, an activin antagonist, ameliorates renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Biomed Res Int. 2014;2014:376191. Figure 5(a)

Image from Gawlik KI, Holmberg J, Svensson M, Einerborg M, Oliveira BM, Deierborg T. Potent pro-inflammatory and pro-fibrotic molecules, osteopontin and galectin-3, are not major disease modulators of laminin α2 chain-deficient muscular dystrophy. Sci Rep. 2017;10:1038. Figure 6(a)

Specificity	Reacts with conformational determinants on type III collagen
Source	Pooled antisera from goats hyperimmunized with type III collagen
Cross Adsorption	Collagen types I, II, IV, V, and VI
Purification	Affinity chromatography on type III collagen covalently linked to agarose
Isotype	Goat IgG
Isotype Control	Goat IgG-UNLB
Format/Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Concentration	0.4 mg/mL
Volume	0.5 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA ^1-3 Other referenced applications for relevant formats include – Immunohistochemistry-Paraffin Sections ^4-20 Immunohistochemistry-Frozen Sections ^{6,9,12,15,21-31} Immunocytochemistry ^9,32,33 Electron Microscopy ^6,12,34,35 Flow Cytometry ^36,37 Western Blot ^{4,10,13,14,22,38,39}
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2794734

Gene ID	1281 (Human)
Gene Symbol	COL3A1 (Human)
Gene Aliases	EDS4A; EDSVASC; PMGEDSV
Uniprot ID	P02461 (Human)
Uniprot Name	CO3A1_HUMAN

References

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