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Goat Anti-Type III Collagen-UNLB抗體 | 貨號1330-01

Primary Antibodies
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https://www.southernbiotech.com
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Goat Anti-Type III Collagen-UNLB抗體抗體操作手冊

特點

Goat Anti-Type III Collagen-UNLB抗體 | 貨號1330-01  規格200ug  (0.4 mg/mL)

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質一級抗體及二級抗體,目前除了一般常見的多株與單株抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。

產品介紹:  山羊抗III型膠原蛋白-UNLB抗體,不具標誌。針對type III collagen具特異性。來源為Pooled antisera from goats hyperimmunized with type III collagen。Isotype為Goat IgG。Isotype Control Goat IgG-UNLB。 緩衝液配方在Borate buffered saline, pH 8.2。

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.

Frozen osteopontin-null mouse muscle section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.

Paraplast Plus® embedded 2K1C rat kidney section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody and DAB and hematoxylin.

Image from Cuevas CA, Tapia-Rojas C, Cespedes C, Inestrosa NC, Vio CP. β-catenin-dependent signaling pathway contributes to renal fibrosis in hypertensive rats. Biomed Res Int. 2015;2015:726012. Figure 2(e)

Paraplast Plus® is a registered trademark of Leica Biosystems Richmond, Inc.

Frozen kidney section following unilateral ureteral obstruction was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.

Image from Maeshima A, Mishima K, Yamashita S, Nakasatomi M, Miya M, Sakurai T, et al. Follistatin, an activin antagonist, ameliorates renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Biomed Res Int. 2014;2014:376191. Figure 5(a)

Image from Gawlik KI, Holmberg J, Svensson M, Einerborg M, Oliveira BM, Deierborg T. Potent pro-inflammatory and pro-fibrotic molecules, osteopontin and galectin-3, are not major disease modulators of laminin α2 chain-deficient muscular dystrophy. Sci Rep. 2017;10:1038. Figure 6(a)

 

Specificity Reacts with conformational determinants on type III collagen
Source Pooled antisera from goats hyperimmunized with type III collagen
Cross Adsorption Collagen types I, II, IV, V, and VI
Purification Affinity chromatography on type III collagen covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Format/Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Concentration 0.4 mg/mL
Volume 0.5 mL
Storage & Handling 2-8°C Please refer to product specific SDS
Applications

Quality tested applications for relevant formats include –

ELISA 1-3

Other referenced applications for relevant formats include –

Immunohistochemistry-Paraffin Sections 4-20 Immunohistochemistry-Frozen Sections 6,9,12,15,21-31 Immunocytochemistry 9,32,33 Electron Microscopy 6,12,34,35 Flow Cytometry 36,37 Western Blot 4,10,13,14,22,38,39

Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2794734

 

Gene ID 1281 (Human)
Gene Symbol COL3A1 (Human)
Gene Aliases EDS4A; EDSVASC; PMGEDSV
Uniprot ID P02461 (Human)
Uniprot Name CO3A1_HUMAN

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

References

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2.Kottler UB, Jünemann AG, Aigner T, Zenkel M, Rummelt C, Schlötzer-Schrehardt U. Comparative effects of TGF-β1 and TGF-β2 on extracellular matrix production, proliferation, migration, and collagen contraction of human Tenon’s capsule fibroblasts in pseudoexfoliation and primary open-angle glaucoma. Exp Eye Res. 2005;80:121-34. (ELISA, Human Reactivity)

3.Will TA, Polcari AJ, Garcia JG, Ouwenga MK, Voelzke BB, Greisler HP, et al. Paclitaxel inhibits ureteral smooth muscle cell proliferation and collagen production in the absence of cell toxicity. J Urol. 2011;185:335-40. (ELISA, Canine Reactivity)

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6.Leite VH, Croft SL. Hepatic extracellular matrix in BALB/c mice infected with Leishmania donovani. Int J Exp Pathol. 1996;77:181-90. (IHC-PS, IHC-FS, EM, Mouse Reactivity)

7.Hamaya M, Takahashi I, Sasano Y, Kuwabara M, Iijima M, Hayashi K, et al. An immunohistochemical study of the localization of types I, II and III collagen in the temporomandibular joint of growing monkeys. Acta Histochem Cytochem. 2001;34:405-11. (IHC-PS, Rhesus Rectivity)

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11.Saika S, Miyamoto T, Tanaka T, Ohnishi Y, Ooshima A, Kimura W. Histopathology of anterior lens capsules in vitrectomized eyes with tamponade by silicone oil. J Cataract Refract Surg. 2002;28:376-8. (IHC-PS, Human Reactivity)

12.Heidet L, Bongers EM, Sich M, Zhang S, Loirat C, Meyrier A, et al. In vivo expression of putative LMX1B targets in nail-patella syndrome kidneys. Am J Pathol. 2003;163:145-55. (IHC-PS, IHC-FS, EM, Human Reactivity)

13.Smeulders N, Woolf AS, Wilcox DT. Extracellular matrix protein expression during mouse detrusor development. J Pediatr Surg. 2003;38:1-12. (IHC-PS, WB, Mouse Reactivity)

14.Hou C, Wang W, Huang XR, Fu P, Chen T, Sheikh-Hamad D, et al. Ultrasound-microbubble-mediated gene transfer of inducible Smad7 blocks transforming growth factor-β signaling and fibrosis in rat remnant kidney. Am J Pathol. 2005;166:761-71. (IHC-PS, WB, Rat Reactivity)

15.Egging DF, van Vlijmen I, Starcher B, Gijsen Y, Zweers MC, Blankevoort L, et al. Dermal connective tissue development in mice: an essential role for tenascin-X. Cell Tissue Res. 2006;323:465-74. (IHC-PS, IHC-FS, Mouse Reactivity)

16.De Philippo RE, Bishop CE, Filho LF, Yoo JJ, Atala A. Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. Transplantation. 2008;86:208-14. (IHC-PS, Rabbit Reactivity)

17.Merrett K, Fagerholm P, McLaughlin CR, Dravida S, Lagali N, Shinozaki N, et al. Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen. Invest Ophthalmol Vis Sci. 2008;49:3887-94. (IHC-PS, Porcine Reactivity)

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19.Inui A, Kokubu T, Fujioka H, Nagura I, Sakata R, Nishimoto H, et al. Application of layered poly (L-lactic acid) cell free scaffold in a rabbit rotator cuff defect model. Sports Med Arthrosc Rehabil Ther Technol. 2011;3:29. (IHC-PS, Rabbit Reactivity)

20.Oosterhuis NR, Frenay AS, Wesseling S, Snijder PM, Slaats GG, Yazdani S, et al. DL-propargylglycine reduces blood pressure and renal injury but increases kidney weight in angiotensin-II infused rats. Nitric Oxide. 2015;49:56-66. (IHC-PS, Rat Reactivity)

21.Jerdan JA, Michels RG, Glaser BM. Extracellular matrix of newly forming vessels—an immunohistochemical study. Microvasc Res. 1991;42:255-65. (IHC-FS, Guinea Pig & Rabbit Reactivity)

22.Tidball JG. Distribution of collagens and fibronectin in the subepicardium during avian cardiac development. Anat Embryol. 1992;185:155-62. (IHC-FS, WB, Chicken Reactivity)

23.Miyaishi O, Sakata K, Matsuyama M, Saga S. Distribution of the collagen binding heat-shock protein in chicken tissues. J Histochem Cytochem. 1992;40:1021-9. (IHC-FS, Chicken Reactivity)

24.Dixon IM, Ju H, Reid NL, Scammell-La Fleur T, Werner JP, Jasmin G. Cardiac collagen remodeling in the cardiomyopathic Syrian hamster and the effect of losartan. J Mol Cell Cardiol. 1997;29:1837-50. (IHC-FS, Hamster Reactivity)

25.Birch HL, Bailey AJ, Goodship AE. Macroscopic ‘degeneration’ of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition. Equine Vet J. 1998;30:534-9. (IHC-FS, Equine Reactivity)

26.Harada T, Juhn SK, Kim Y, Sakakura Y. Immunohistochemical distribution of extracellular matrix components and keratin in experimentally induced otitis media. Ann Otol Rhinol Laryngol. 1999;108:769-76. (IHC-FS, Chinchilla Reactivity)

27.Boos A, Stelljes A, Kohtes J. Collagen types I, III and IV in the placentome and interplacentomal maternal and fetal tissues in normal cows and in cattle with retention of fetal membranes. Cells Tissues Organs. 2003;174:170-83. (IHC-FS, Bovine Reactivity)

28.Albon J, Farrant S, Akhtar S, Young R, Boulton ME, Smith G, et al. Connective tissue structure of the tree shrew optic nerve and associated ageing changes. Invest Ophthalmol Vis Sci. 2007;48:2134-44. (IHC-FS, Tree Shrew Reactivity)

29.Chun SY, Lim GJ, Kwon TG, Kwak EK, Kim BW, Atala A, et al. Identification and characterization of bioactive factors in bladder submucosa matrix. Biomaterials. 2007;28:4251-6. (IHC-FS, Porcine Reactivity)

30.Sánchez-Lara AC, Elliott J, Syme HM, Brown CA, Haylor JL. Feline chronic kidney disease is associated with upregulation of transglutaminase 2: a collagen cross-linking enzyme. Vet Pathol. 2015;52:513-23. (IHC-FS, Feline Reactivity)

31.Starokozhko V, Abza GB, Maessen HC, Merema MT, Kuper F, Groothuis GM. Viability, function and morphological integrity of precision-cut liver slices during prolonged incubation: Effects of culture medium. Toxicol In Vitro. 2015;30:288-99. (IHC-FS, Rat Reactivity)

32.Simmons PJ, Przepiorka D, Thomas ED, Torok-Storb B. Host origin of marrow stromal cells following allogeneic bone marrow transplantation. Nature. 1987;328:429-32. (ICC, Human Reactivity)

33.Karamichos D, Hutcheon AE, Zieske JD. Reversal of fibrosis by TGF-β3 in a 3D in vitro model. Exp Eye Res. 2014;124:31-6. (ICC, Human Reactivity)

34.Gorgels TG, Teeling P, Meeldijk JD, Nillesen ST, van der Wal AC, van Kuppevelt TH, et al. Abcc6 deficiency in the mouse leads to calcification of collagen fibers in Bruch’s membrane. Exp Eye Res. 2012;104:59-64. (EM, Mouse Reactivity)

35.Dingemans KP, Teeling P, Lagendijk JH, Becker AE. Extracellular matrix of the human aortic media: an ultrastructural histochemical and immunohistochemical study of the adult aortic media. Anat Rec. 2000;258:1-14. (EM, Human Reactivity)

36.Xu S, Vancheeswaran R, Bou-Gharios G, O’Hare MJ, Olsen I, Abraham D, et al. Scleroderma-derived human fibroblasts retain abnormal phenotypic and functional characteristics following retroviral transduction with the SV40 tsT antigen. Exp Cell Res. 1995;220:407-14. (FC, Human Reactivity)

37.Gopaul R, Knaggs HE, Lephart ED. Biochemical investigation and gene analysis of equol: a plant and soy-derived isoflavonoid with antiaging and antioxidant properties with potential human skin applications. Biofactors. 2012;38:44-52. (FC, Human Reactivity)

38.Strauss BH, Robinson R, Batchelor WB, Chisholm RJ, Ravi G, Natarajan MK, et al. In vivo collagen turnover following experimental balloon angioplasty injury and the role of matrix metalloproteinases. Circ Res. 1996;79:541-50. (WB, Rabbit Reactivity)

39.Zhou Q, Xiong Y, Huang XR, Tang P, Yu X, Lan HY. Identification of genes associated with Smad3-dependent renal injury by RNA-seq-based transcriptome analysis. Sci Rep. 2015;5:17901. (WB, Mouse Reactivity)

 

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