Goat Anti-Mouse IgG2c, Human ads-Alexa Fluor® 647抗體 | 貨號1079-31
Goat Anti-Mouse IgG2c, Human ads-Alexa Fluor® 647抗體 | 貨號1079-31 規格1.0 mg (1.0 mg/mL)
美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。
二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。
FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2c, Human ads-AF647 (SB Cat. No. 1079-31).
產品介紹: Goat Anti-Mouse IgG2c, Human ads-Alexa Fluor® 647抗體，具Alexa Fluor® 647標誌。針對C57BL/6 mouse IgG2c重鏈具特異性。來源為goats抗血清。Isotype為Goat IgG。Isotype Control Goat IgG-Alexa Fluor® 647。 緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。
| Specificity | Reacts with the heavy chain of C57BL/6 mouse IgG2c |
| Source | Pooled antisera from goats hyperimmunized with mouse IgG2c |
| Cross Adsorption | Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species |
| Purification | Affinity chromatography on mouse IgG2c covalently linked to agarose |
| Isotype | Goat IgG |
| Isotype Control | Goat IgG-Alexa Fluor® 647 |
| Format/Conjugate | AF647 (Alexa Fluor® 647) |
| Buffer Formulation | Phosphate buffered saline containing 0.1% sodium azide |
| Concentration | 1.0 mg/mL |
| Volume | 1.0 mL |
| Storage & Handling | 2-8°C; Avoid exposure to light Please refer to product specific SDS |
| Applications |
Quality tested applications for relevant formats include – ELISA 1-20 Other referenced applications for relevant formats include – Flow Cytometry 21,22,25 |
| Recommended Dilutions | Please refer to product specific Technical Bulletin |
| RRID | AB_2794473 |
SBA熱銷商品 7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 | DAPI螢光封片劑 | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I | 一級抗體