表皮生長因子EFG重組蛋白 | 貨號Z100135

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表皮生長因子EFG重組蛋白 | 貨號Z100135

Recombinant Human EGF

產品描述: 表皮生長因子（Epidermal growth factor , EGF）是一種小的促有絲分裂蛋白(mitogenic protein)，被認為與正常細胞生長(normal cell growth)，腫瘤發生(oncogenesis)和傷口癒合(wound healing)等機制有關。該蛋白與人類E型轉化生長因子（human type-alpha transforming growth factor , hTGFα）表現出很強的序列(strong sequential)和功能同源性(functional homology)，後者是EGF受體位點(EGF receptor sites)的競爭者。 EGF受體在幾乎所有類型的組織中都有表達。頂葉內胚層，成熟的骨骼肌和造血組織不表達該受體。 EGF受體稱為HER1，是一種170 kDa跨膜糖蛋白，長度為1186個氨基酸。它與先前描述的稱為SA-7（物種抗原7）的糖蛋白相同。細胞外受體結構域具有621個氨基酸的長度，包括11個糖基化的天冬酰胺殘基和51個半胱氨酸殘基。該結構域包含EGF結合位點，並且還結合哺乳動物TGF-α。

SKU	Z100135
Growth Factor Size	500 µg
Unit quantity	500 µg
Family	EGF Family
Aliases	Urogastrone, EGF Recombinant Human Epidermal Growth Factor (EGF)
Gene Symbol	EGF
Gene ID	1950
Accession Number	P01133
Source	E. coli
Species	Human
Appearance	Lyophilized Powder
Molecular Weight	6.5 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Epidermal Growth Factor was lyophilized from a 0.2 μm filtered solution in PBS.
Function	The ED50 was determined by a cell proliferation assay using human epithelial cell line and found to be <0.2ng/ml.
Reconstitution	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° – 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Description	Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites. EGF receptors are expressed in almost all types of tissues. Parietal endoderm, mature skeletal muscles, and hematopoietic tissues do not express the receptor. The EGF receptor, designated HER1, is a 170 kDa transmembrane glycoprotein with a length of 1186 amino acids. It is identical with a previously described glycoprotein called SA-7 (species antigen 7). The extracellular receptor domain has a length of 621 amino acids, including 11 glycosylated asparagine residues and 51 cysteine residues. This domain contains the EGF binding site and also binds mammalian TGF-alpha.

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