Mouse Anti-Human IgA1-BIOT抗體 | Southern Biotech貨號9130-08

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特點

Mouse Anti-Human IgA1-BIOT抗體 | 貨號9130-08規格1.0 mL (0.5 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

ELISA plate was coated with purified human IgA1, IgA2, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA1-BIOT (SB Cat. No. 9130-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

產品介紹: Mouse Anti-Human IgA1-BIOT抗體，具BIOTIN標誌。針對Fc fragment of human IgA₁ myeloma protein具特異性。

Isotype為Mouse IgG₁κ。Isotype ControlMouse IgG₁-BIOT。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。

Clone	B3506B4
Isotype	Mouse IgG₁κ
Isotype Control	Mouse IgG₁-BIOT
Immunogen	Fc fragment of human IgA₁ myeloma protein
Specificity	Human IgA₁ Fc
Format/Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	0.5 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Applications for relevant formats of this clone include – ELISA – Quality tested ^1-9 FLISA – Quality tested ELISpot – Reported in literature ¹⁰ Flow Cytometry – Reported in literature ^6,16 Immunohistochemistry-Frozen Sections – Reported in literature ^6,11 Immunocytochemistry – Reported in literature ^6,12 Western Blot – Reported in literature ^13-15 Multiplex – Reported in literature ^17-20 Separation – Reported in literature ⁶ Depletion – Reported in literature ²¹
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2796655

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