Goat Anti-Mouse IgG(H+L), Human ads-AP抗體| 貨號1031-04

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原廠連結：	https://www.southernbiotech.com
相關下載：	Goat Anti-Mouse IgG(H+L), Human ads-AP抗體操作手冊

特點

Goat Anti-Mouse IgG(H+L), Human ads-AP抗體 | 貨號1031-04 規格1.0 ml

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-TRITC (SB Cat. No. 1031-03) and DAPI.

產品介紹: Goat Anti-Mouse IgG(H+L), Human ads-AP抗體，具AP (Alkaline Phosphatase)標誌。針對mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3輕鏈及重鏈以及mouse IgM and IgA輕鏈具特異性。Pooled antisera from goats hyperimmunized with mouse IgG。Isotype: Goat IgG。緩衝液配方在50 mM Tris/1 mM MgCl₂/50% Glycerol containing 0.1% sodium azide, pH 8.0。

Specificity	Reacts with the heavy and light chains of mouse IgG₁, IgG_2a, IgG_2b, IgG_2c, and IgG₃ and with the light chains of mouse IgM and IgA
Source	Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption	Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification	Affinity chromatography on mouse IgG covalently linked to agarose
Isotype	Goat IgG
Format/Conjugate	AP (Alkaline Phosphatase)
Buffer Formulation	50 mM Tris/1 mM MgCl₂/50% Glycerol containing 0.1% sodium azide, pH 8.0
Volume	1.0 mL
Storage & Handling	2-8°C Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA ^1-7 FLISA ⁸ Flow Cytometry ^3,8-13 Other referenced applications for relevant formats include – ELISpot ²⁸ Immunohistochemistry-Frozen Sections ^14,15 Immunohistochemistry-Paraffin Sections ¹⁶ Immunocytochemistry ^12,17-20 Western Blot ^21-27
Recommended Dilutions	Please refer to product specific Technical Bulletin

引用文獻

References

Swiecki MK, Lisanby MW, Shu F, Turnbough CL Jr, Kearney JF. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J Immunol. 2006;176:6076-84. (ELISA)
Stegmann T, Kamphuis T, Meijerhof T, Goud E, de Haan A, Wilschut J. Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation. Vaccine. 2010;28:5543-50. (ELISA)
Christianson GJ, Sun VZ, Akilesh S, Pesavento E, Proetzel G, Roopenian DC. Monoclonal antibodies directed against human FcRn and their applications. MAbs. 2012;4:208-16. (ELISA, FC)
Stefka AT, Feehley T, Tripathi P, Qiu J, McCoy K, Mazmanian SK, et al. Commensal bacteria protect against food allergen sensitization. Proc Natl Acad Sci USA. 2014;111:13145-50. (ELISA)
Silva-Sanchez A, Liu CR, Vale AM, Khass M, Kapoor P, Elgavish A, et al. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. 2015;10(2):e0118171. (ELISA)
Lascano V, Hahne M, Papon L, Cameron K, Röeder C, Schafmayer C, et al. Circulating APRIL levels are correlated with advanced disease and prognosis in rectal cancer patients. Oncogenesis. 2015;4:e136. (ELISA)
Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)
Kilpatrick KE, Cutler T, Whitehorn E, Drape RJ, Macklin MD, Witherspoon SM, et al. Gene gun delivered DNA-based immunizations mediate rapid production of murine monoclonal antibodies to the Flt-3 receptor. Hybridoma. 1998;17:569-76. (FLISA, FC)
Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J Immunol. 2005;175:7325-31. (FC)
Donini M, Zenaro E, Tamassia N, Dusi S. NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206. Eur J Immunol. 2007;37:1194-203. (FC)
Delaleu N, Madureira AC, Immervoll H, Jonsson R. Inhibition of experimental Sjögren’s syndrome through immunization with HSP60 and its peptide amino acids 437-460. Arthritis Rheum. 2008;58:2318-28. (FC)
Le TL, Mironova E, Garcin D, Compans RW. Induction of influenza-specific mucosal immunity by an attenuated recombinant Sendai virus. PLoS One. 2011;6:e18780. (FC, ICC)
Daflon-Yunes N, Pinto-Silva FE, Vidal RS, Novis BF, Berguetti T, Lopes RR, et al. Characterization of a multidrug-resistant chronic myeloid leukemia cell line presenting multiple resistance mechanisms. Mol Cell Biochem. 2013;383:123-35. (FC)
Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (IHC-FS)
Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (IHC-FS)
Deckert V, Kretz B, Habbout A, Raghay K, Labbé J, Abello N, et al. Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency. Am J Pathol. 2013;183:975-86. (IHC-PS)
Scaife S, Brown R, Kellie S, Filer A, Martin S, Thomas AM, et al. Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display. Rheumatology. 2004;43:1346-52. (ICC)
Thomas RA, Norman JC, Huynh TT, Williams B, Bolton SJ, Wardlaw AJ. Mechanical stretch has contrasting effects on mediator release from bronchial epithelial cells, with a rho-kinase-dependent component to the mechanotransduction pathway. Respir Med. 2006;100:1588-97. (ICC)
Lee S, Bahn JH, Choi CK, Whitlock NC, English AE, Safe S, et al. ESE-1/EGR-1 pathway plays a role in tolfenamic acid-induced apoptosis in colorectal cancer cells. Mol Cancer Ther. 2008;7:3739-50. (ICC)
Tarafdar S, Poe JA, Smithgall TE. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28. (ICC)
Dalen KT, Ulven SM, Arntsen BM, Solaas K, Nebb HI. PPARα activators and fasting induce the expression of adipose differentiation-related protein in liver. J Lipid Res. 2006;47:931-43. (WB)
Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (WB)
Lunde IG, Kvaløy H, Austbø B, Christensen G, Carlson CR. Angiotensin II and norepinephrine activate specific calcineurin-dependent NFAT transcription factor isoforms in cardiomyocytes. J Appl Physiol. 2011;111:1278-89. (WB)
Norheim F, Raastad T, Thiede B, Rustan AC, Drevon CA, Haugen F. Proteomic identification of secreted proteins from human skeletal muscle cells and expression in response to strength training. Am J Physiol Endocrinol Metab. 2011;301:E1013-21. (WB)
Thomas AP, Dunn TN, Drayton JB, Oort PJ, Adams SH. A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone. Nutr Metab. 2012;9:3. (WB)
Stuckenholz C, Lu L, Thakur PC, Choi T, Shin D, Bahary N. Sfrp5 modulates both Wnt and BMP signaling and regulates gastrointestinal organogenesis in the zebrafish, Danio rerio. PLoS One. 2013;8(4):e62470. (WB)
Muller M, Hutin S, Marigold O, Li KH, Burlingame A, Glaunsinger BA. A ribonucleoprotein complex protects the interleukin-6 mRNA from degradation by distinct herpesviral endonucleases. PLoS Pathog. 2015;11(5):e1004899. (WB)
Rahman ZS, Niu H, Perry D, Wakeland E, Manser T, Morel L. Expression of the autoimmune Fcgr2b NZW allele fails to be upregulated in germinal center B cells and is associated with increased IgG production. Genes Immun. 2007;8:604-12. (ELISPOT)

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