PCA3 ORF Vector (Human) (pORF) | 貨號ORF027448 (1.0 µg DNA)

Abmgood提供一系列序列驗證開放閱讀框（ORF）庫，包括人(human)，小鼠(mouse)和大鼠(rat)，可insertion至您選擇的蛋白質表達系統(protein expression systems.)。去除5’UTR和3’UTR，ORF插入兼容的表達載體(compatible expression vector) 後即可快速可靠地表達蛋白質。也可用作PCR擴增 (PCR amplification experiments) 實驗中陽性對照(positive control)的模板(template)。Abmgood提供的ORF包含一個突變的終止密碼子(mutated stop codon)，如果受體載體中存在C末端標籤或報告基因，則允許其摻入。 abm的ORF也可以以即用型蛋白表達載體(protein expression vectors)，慢病毒載體(lentiviral vectors)或包裝的慢病毒( lentivirus)和腺病毒( adenovirus )之架構好之載體或病毒液形式提供。

PCA3 ORF載體介紹: Accession Number NR_015342 架構
pORF。 PCA3 ORF載體在AflII和EcoRV切割位點之間保留了該PCA3 基因（限制性酶切方法）。Inserts不包含任何內部AflII和EcoRV cut site，可以AflII和EcoRV切除。在插入片段包含這些內部切割位點的情況下，可以PCR擴增帶有優選限制性酶切位點的插入片段，以進行subcloning。

Gene Insert	PCA3
Description	The PCA3 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.
Accession Number	NR_015342
Vector	pORF
Vector Size	2596bp
Insert Size	3735bp
Species	Human
Selection Marker	Bacterial: Spectinomycin
Appearance	Clear Liquid
Storage Buffer	10mM Tris-HCI, 1mM EDTA, pH8.0
Stability	1 year when stored at -20°C or lower in a non-frost free freezer.
Caution	PCA3 ORF Vector (Human) (pORF) is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

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