Goat Anti-Mouse IgG1, Human ads-PE抗體 | 貨號1070-09S 規格0.5ml  (0.5 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

FLISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG1, Human ads-PE (SB Cat. No. 1070-09S).

產品介紹:  Goat Anti-Mouse IgG1, Human ads-PE抗體，具PE標誌。針對heavy chain of mouse IgG₁具特異性。Isotype為Goat IgG。Isotype Control Goat IgG-PE。 緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。

Specificity	Reacts with the heavy chain of mouse IgG1
Source	Pooled antisera from goats hyperimmunized with mouse IgG1
Cross Adsorption	Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification	Affinity chromatography on mouse IgG1 covalently linked to agarose
Isotype	Goat IgG
Isotype Control	Goat IgG-PE
Format/Conjugate	PE (R-phycoerythrin)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide and a stabilizer
Concentration	0.5 mg/mL
Volume	0.5 mL
Storage & Handling	2-8°C; Avoid exposure to light; Do not freeze Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA 1-8 FLISA Flow Cytometry 6,9-15 Other referenced applications for relevant formats include – ELISpot 4,6,7 Immunohistochemistry-Frozen Sections 3,9,16,17 Immunohistochemistry-Paraffin Sections 1,16,18,19 Immunohistochemistry-Whole Mount 25 Immunocytochemistry 14,20,21 Electron Microscopy 17 Western Blot 2,5,13,22,23 Surface Plasmon Resonance 24 Multiplex 26
Recommended Dilutions	Please refer to product specific Technical Bulletin

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

引用文獻

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