Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體 | SBA貨號4050-30

Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體 | 貨號4050-30 規格1.0 mg (1.0 mg/mL)
美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。
二級抗體 (Secondary antibodies) 應用:
二抗(Secondary antibodies)是針對免疫球蛋白(immunoglobulin , Ig)分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用,並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用,例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記,這使得能夠通過比色,化學發光和螢光技術進行檢測。例如,酶辣根過氧化物酶(enzymes horseradish peroxidase , HRP)和鹼性磷酸酶(alkaline phosphatase , AP)通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素(fluorescein , FITC),藻紅蛋白(phycoerythrin , PE)和花青(cyanine , CY)的螢光染料在螢光的應用中是必不可少的,包括流式細胞術,免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大,可增加的靈敏度。

產品介紹: Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體,具Alexa Fluor® 488標誌。針對rabbit IgG輕鏈及重鏈以及rabbit IgM輕鏈具特異性。來源為Pooled antisera from goats hyperimmunized with rabbit IgG。Isotype為Goat IgG。Isotype Control Goat IgG-Alexa Fluor® 488。 緩衝液配方在Phosphate buffered saline 含有0.1% sodium azide防腐劑。
| Specificity | Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM |
| Source | Pooled antisera from goats hyperimmunized with rabbit IgG |
| Cross Adsorption | Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species |
| Purification | Affinity chromatography on pooled rabbit IgG covalently linked to agarose |
| Isotype | Goat IgG |
| Isotype Control | Goat IgG-Alexa Fluor® 488 |
| Format/Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing 0.1% sodium azide |
| Concentration | 1.0 mg/mL |
| Volume | 1.0 mL |
| Storage & Handling | 2-8°C; Avoid exposure to light Please refer to product specific SDS |
| Applications |
Quality tested applications for relevant formats include – ELISA 1-3 Other referenced applications for relevant formats include – Immunohistochemistry-Frozen Sections 8-10 |
| Recommended Dilutions | Please refer to product specific Technical Bulletin |
| RRID | AB_2795961 |
SBA熱銷商品 7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 | DAPI螢光封片劑 | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I | 一級抗體
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