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Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體 | SBA貨號4050-30

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Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體操作手冊

特點

Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體 | 貨號4050-30 規格1.0 mg  (1.0 mg/mL)

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白(immunoglobulin , Ig)分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用,並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用,例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記,這使得能夠通過比色,化學發光和螢光技術進行檢測。例如,酶辣根過氧化物酶(enzymes horseradish peroxidase , HRP)和鹼性磷酸酶(alkaline phosphatase , AP)通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素(fluorescein , FITC),藻紅蛋白(phycoerythrin , PE)和花青(cyanine , CY)的螢光染料在螢光的應用中是必不可少的,包括流式細胞術,免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大,可增加的靈敏度。

Paraffin embedded NB-9464 induced mouse tumor tissue was stained with anti-KI-67 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05), DAB, and hematoxylin. Image from Sun M, Ha N, Pham D, Frederick M, Sharma B, Naruse C, et al. Cbx3/HP1γ deficiency confers enhanced tumor-killing capacity on CD8+ T cells. Sci Rep. 2017;7:42888. Figure 2(f)

產品介紹:  Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-Alexa Fluor® 488抗體,具Alexa Fluor® 488標誌。針對rabbit IgG輕鏈及重鏈以及rabbit IgM輕鏈具特異性。來源為Pooled antisera from goats hyperimmunized with rabbit IgG。Isotype為Goat IgG。Isotype Control Goat IgG-Alexa Fluor® 488。 緩衝液配方在Phosphate buffered saline 含有0.1% sodium azide防腐劑。

Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Mouse and human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Isotype Goat IgG
Isotype Control Goat IgG-Alexa Fluor® 488
Format/Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing 0.1% sodium azide
Concentration 1.0 mg/mL
Volume 1.0 mL
Storage & Handling 2-8°C; Avoid exposure to light
Please refer to product specific SDS
Applications

Quality tested applications for relevant formats include –

ELISA 1-3
FLISA
Flow Cytometry 4-7

Other referenced applications for relevant formats include –

Immunohistochemistry-Frozen Sections 8-10
Immunohistochemistry-Paraffin Sections 6,11-13
Immunohistochemistry-Whole Mount 14,15
Immunocytochemistry 16-19
Western Blot 20-28
Separation 29

Recommended Dilutions Please refer to product specific Technical Bulletin
RRID AB_2795961

 SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

References

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  2. Zhang X, He M, Cheng L, Chen Y, Zhou L, Zeng H, et al. Elevated heat shock protein 60 levels are associated with higher risk of coronary heart disease in Chinese. Circulation. 2008;118:2687-93. (ELISA)
  3. Jin K, Wang S, Zhang C, Xiao Y, Lu S, Huang Z. Protective antibody responses against Clostridium difficile elicited by a DNA vaccine expressing the enzymatic domain of toxin B. Hum Vaccin Immunother. 2013;9:63-73. (ELISA)
  4. Burger PE, Gupta R, Xiong X, Ontiveros CS, Salm SN, Moscatelli D, et al. High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells. Stem Cells. 2009;27:2220-8. (FC)
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  6. Aziz M, Yang W, Matsuo S, Sharma A, Zhou M, Wang P. Upregulation of GRAIL is associated with impaired CD4 T cell proliferation in sepsis. J Immunol. 2014;192:2305-14. (FC, IHC-PS)
  7. Boonstra MC, Tolner B, Schaafsma BE, Boogerd LS, Prevoo HA, Bhavsar G, et al. Preclinical evaluation of a novel CEA-targeting near-infrared fluorescent tracer delineating colorectal and pancreatic tumors. Int J Cancer. 2015;137:1910-20. (FC)
  8. Borrego L, Maynard B, Peterson EA, George T, Iglesias L, Peters MS, et al. Deposition of eosinophil granule proteins precedes blister formation in bullous pemphigoid. Comparison with neutrophil and mast cell granule proteins. Am J Pathol. 1996;148:897-909. (IHC-FS)
  9. Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW. Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-β1-mediated conversion of fibroblasts to myofibroblasts. Am J Pathol. 2002;161:471-80. (IHC-FS)
  10. Xia RH, Yosef N, Ubogu EE. Clinical, electrophysiological and pathologic correlations in a severe murine experimental autoimmune neuritis model of Guillain-Barré syndrome. J Neuroimmunol. 2010;219:54-63. (IHC-FS)
  11. Penkowa M, Carrasco J, Giralt M, Molinero A, Hernández J, Campbell IL, et al. Altered central nervous system cytokine-growth factor expression profiles and angiogenesis in metallothionein-I+II deficient mice. J Cereb Blood Flow Metab. 2000;20:1174-89. (IHC-PS)
  12. He X, Schoeb TR, Panoskaltsis-Mortari A, Zinn KR, Kesterson RA, Zhang J, et al. Deficiency of P-selectin or P-selectin glycoprotein ligand-1 leads to accelerated development of glomerulonephritis and increased expression of CC chemokine ligand 2 in lupus-prone mice. J Immunol. 2006;177:8748-56. (IHC-PS)
  13. Rossato E, Mkaddem SB, Kanamaru Y, Hurtado-Nedelec M, Hayem G, Descatoire V, et al. Reversal of arthritis by human monomeric IgA through the receptor-mediated SH2 domain-containing phosphatase 1 inhibitory pathway. Arthritis Rheumatol. 2015;67:1766-77. (IHC-PS)
  14. Hens J, Gajda M, Scheuermann DW, Adriaensen D, Timmermans J. The longitudinal smooth muscle layer of the pig small intestine is innervated by both myenteric and submucous neurons. Histochem Cell Biol. 2002;117:481-92. (IHC-WM)
  15. Monte E, Mouillesseaux K, Chen H, Kimball T, Ren S, Wang Y, et al. Systems proteomics of cardiac chromatin identifies nucleolin as a regulator of growth and cellular plasticity in cardiomyocytes. Am J Physiol Heart Circ Physiol. 2013;305:H1624-38. (IHC-WM)
  16. Roberts MS, Woods AJ, Dale TC, van der Sluijs P, Norman JC. Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of αvβ3 and α5β1 integrins. Mol Cell Biol. 2004;24:1505-15. (ICC)
  17. Luijten MN, Basten SG, Claessens T, Vernooij M, Scott CL, Janssen R, et al. Birt-Hogg-Dubé syndrome is a novel ciliopathy. Hum Mol Genet. 2013;22:4383-97. (ICC)
  18. Tarafdar S, Poe JA, Smithgall TE. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28. (ICC)
  19. Carey AJ, Tan CK, Mirza S, Irving-Rodgers H, Webb RI, Lam A, et al. Infection and cellular defense dynamics in a novel 17β-estradiol murine model of chronic human group B streptococcus genital tract colonization reveal a role for hemolysin in persistence and neutrophil accumulation. J Immunol. 2014;192:1718-31. (ICC)
  20. Chen Z, Fu L, Herrero R, Schiffman M, Burk RD. Identification of a novel human papillomavirus (HPV97) related to HPV18 and HPV45. Int J Cancer. 2007;121:193-8. (WB)
  21. Iuchi K, Hatano Y, Yagura T. Heterocyclic organobismuth(III) induces apoptosis of human promyelocytic leukemic cells through activation of caspases and mitochondrial perturbation. Biochem Pharmacol. 2008;76:974-86. (WB)
  22. Fadnes B, Husebekk A, Svineng G, Rekdal Ø, Yanagishita M, Kolset SO, et al. The proteoglycan repertoire of lymphoid cells. Glycoconj J. 2012;29:513-23. (WB)
  23. Tong J, Meyer JH, Furukawa Y, Boileau I, Chang L, Wilson AA, et al. Distribution of monoamine oxidase proteins in human brain: implications for brain imaging studies. J Cereb Blood Flow Metab. 2013;33:863-71. (WB)
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  26. Goss JW, Kim S, Bledsoe H, Pollard TD. Characterization of the roles of Blt1p in fission yeast cytokinesis. Mol Biol Cell. 2014;25:1946-57. (WB)
  27. Hogan MC, Bakeberg JL, Gainullin VG, Irazabal MV, Harmon AJ, Lieske JC, et al. Identification of biomarkers for PKD1 using urinary exosomes. J Am Soc Nephrol. 2015;26:1661-70. (WB)
  28. Graf FE, Baker N, Munday JC, de Koning HP, Horn D, Mäser P. Chimerization at the AQP2-AQP3 locus is the genetic basis of melarsoprol-pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates. Int J Parasitol Drugs Drug Resist. 2015;5:65-8. (WB)
  29. Semba K, Namekata K, Guo X, Harada C, Harada T, Mitamura Y. Renin-angiotensin system regulates neurodegeneration in a mouse model of normal tension glaucoma. Cell Death Differ. 2014;5:e1333. (Sep)

Alexa Fluor® is a registered trademark of Life Technologies Corporation

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