Goat Anti-Rabbit IgG-FITC抗體 | 貨號4030-02

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原廠連結：	https://www.southernbiotech.com
相關下載：	Goat Anti-Rabbit IgG-FITC抗體操作手冊

特點

Goat Anti-Rabbit IgG-FITC抗體 | 貨號4030-02 規格1.0 ml (1 mg/mL)

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體，目前除了一般常見的多株與單株二級抗體外，還帶有螢光物質（FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）、酵素（HRP、AP）、Low Endotoxin/Azide Free（LE/AF）等標定，以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品，協助各種實驗進行。

二級抗體 (Secondary antibodies) 應用:

二抗(Secondary antibodies)是針對免疫球蛋白（immunoglobulin , Ig）分子的多克隆(polyclonal) 和單克隆抗體(monoclonal antibodies)。它們通常與抗原特異性一抗(specific primary antibody) 一起使用，並且通常被標定酵素或螢光物質以幫助檢測。二抗可用於多種應用，例如ELISA、免疫印跡(Immunoblotting)、流式細胞儀(Flow Cytometry)、免疫組織染色(Immunohistochemistry)、免疫細胞化學(Immuocytochemistry)。二級抗體通常用酶或螢光染料標記，這使得能夠通過比色，化學發光和螢光技術進行檢測。例如，酶辣根過氧化物酶（enzymes horseradish peroxidase , HRP）和鹼性磷酸酶（alkaline phosphatase , AP）通過比色和化學發光平台在ELISA和蛋白質印跡應用中是有價值的。螢光蛋白諸如螢光素（fluorescein , FITC），藻紅蛋白（phycoerythrin , PE）和花青（cyanine , CY）的螢光染料在螢光的應用中是必不可少的，包括流式細胞術，免疫螢光顯微術和多重測定。二抗允許標記和檢測的靈活性以及通過信號放大，可增加的靈敏度。

產品介紹: Goat Anti-Rabbit IgG-FITC抗體，具FITC標誌。針對rabbit IgG重鏈具特異性。來源為Pooled antisera from goats hyperimmunized with rabbit IgG。Isotype為Goat IgG。緩衝液配方在Phosphate buffered saline 含有 0.1% sodium azide防腐劑。

Frozen mouse lung tissue section post sensitization with house dust mite extract followed by specific immunotherapy treatment via immunization with house dust mite extract was stained with anti-CRAMP followed by Goat Anti-Rabbit IgG-FITC (SB Cat. No. 4030-02).

Image from Wang J, Liu X, Wang H, Li Y, Ian N, Yuan X, et al. Allergen specific immunotherapy enhanced defense against bacteria via TGF-β1-induced CYP27B1 in asthma. Oncotarget. 2017;8:68681-95. Figure 4(a)

Specificity	Reacts with the heavy chain of rabbit IgG
Source	Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption	Rabbit IgM; may react with immunoglobulins from other species
Purification	Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Isotype	Goat IgG
Isotype Control	Goat IgG-FITC
Format/Conjugate	FITC (Fluorescein)
Buffer Formulation	Phosphate buffered saline containing 0.1% sodium azide
Concentration	1.0 mg/mL
Volume	1.0 mL
Storage & Handling	2-8°C; Avoid exposure to light Please refer to product specific SDS
Applications	Quality tested applications for relevant formats include – ELISA ^1-3 FLISA Other referenced applications for relevant formats include – Flow Cytometry ^4,5 Immunohistochemistry-Frozen Sections ^6-8 Immunohistochemistry-Paraffin Sections ^7,9-11 Immunocytochemistry ^12-14 Western Blot ^15-25
Recommended Dilutions	Please refer to product specific Technical Bulletin
RRID	AB_2795933

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