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Streptavidin-HRP | Streptomyces avidinii貨號7100-05 (貨號異動為7105-05)

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原廠連結:
https://www.southernbiotech.com
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Streptavidin-HRP操作手冊

特點

Streptavidin-HRP | 貨號7100-05  規格1ml (HRP標誌) 

(貨號異動為7105-05)

美國抗體生產商Southern Biotech於1982年,由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體,目前除了一般常見的多株與單株二級抗體外,還帶有螢光物質(FITC、Spectral Red、Alexa 488、Alexa 647、Pacific Blue)、酵素(HRP、AP)、Low Endotoxin/Azide Free(LE/AF)等標定,以及F(ab)和F(ab’)2等抗體片段。諸如此類多樣化產品,協助各種實驗進行。

Paraffin embedded human gastric cancer tissue was stained with Goat Anti-Type I Collagen-BIOT (SB Cat. No. 1310-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.

Paraffin embedded human colon cancer tissue was stained with Mouse Anti-Human MMP-9-UNLB (SB Cat. No. 12025-01) followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.

Paraffin embedded mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08) and then Streptavidin-HRP (SB Cat. No. 7100-05), hematoxylin, and AEC.

Image from van den Borne P, Rygiel TP, Hoogendoorn A, Westerlaken GH, Boon L, Quax PH, et al. The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx. PLoS One. 2014;9(6):e98820. Figure 2(f)

Urine and serum samples were resolved by electrophoresis, transferred to PVDF membrane, and probed with biotinylated anti-human fH followed by Streptavidin-HRP (SB Cat. No. 7100-05) and chemiluminescent detection.
Lane 1 – Purified fH
Lane 2 – Urine from IgAN patient
Lane 3 – Serum from IgAN patient
Lane 4 – Urine from healthy patientImage from Onda K, Ohsawa I, Ohi H, Tamano M, Mano S, Wakabayashi M, et al. Excretion of complement proteins and its activation marker C5b-9 in IgA nephropathy in relation to renal function. BMC Nephrol. 2011;12:64. Figure 4

 

產品介紹:  Streptavidin-HRP,具HRP標誌。針對Biotin具特異性。來源為Streptomyces avidinii。 緩衝液配方在50% Glycerol/50% Phosphate buffered saline, pH 7.4。

Source Streptomyces avidinii
Specificity Biotin
Format/Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Volume 1.0 mL
Storage & Handling 2-8°C
Please refer to product specific SDS
Applications ELISA – Quality tested 1-7
FLISA – Quality tested
Flow Cytometry – Quality tested 1,2,4,8-15
ELISpot – Reported in literature 4,6-8
Immunohistochemistry-Frozen Sections – Reported in literature 9,10,13,16-19
Immunohistochemistry-Paraffin Sections – Reported in literature 16,18,21
Immunocytochemistry – Reported in literature 11,17,19
Western Blot – Reported in literature 3,5,20
Multiplex – Reported in literature 22
Microarray – Reported in literature 23
Blocking – Reported in literature 24
Recommended Dilutions Please refer to product specific Technical Bulletin

SBA熱銷商品  7-AAD細胞活性螢光指示劑 | monocloal isotyping套組 | Annexin V-FITC細胞凋亡檢測 |  DAPI螢光封片劑  | anti-rabbit 二級抗體 | anti-mouse二級抗體 | 膠原蛋白Collagen Type I  | 一級抗體

文獻發表

  1. Hamilton AM, Kearney JF. Effects of IgM allotype suppression on serum IgM levels, B-1 and B-2 cells, and antibody responses in allotype heterozygous F1 mice. Dev Immunol. 1994;4:27-41. (ELISA, FC, IHC-FS)
  2. Gulbranson-Judge A, Tybulewicz VL, Walters AE, Toellner K, MacLennan IC, Turner M. Defective immunoglobulin class switching in Vav-deficient mice is attributable to compromised T cell help. Eur J Immunol. 1999;29:477-87. (ELISA, FC)
  3. Utani A, Nomizu M, Matsuura H, Kato K, Kobayashi T, Takeda U, et al. A unique sequence of the laminin α3 G domain binds to heparin and promotes cell adhesion through syndecan-2 and -4. J Biol Chem. 2001;276:28779-88. (ELISA, WB)
  4. Taylor DK, Ito E, Thorn M, Sundar K, Tedder T, Spatz LA. Loss of tolerance of anti-dsDNA B cells in mice overexpressing CD19. Mol Immunol. 2006;43:1776-90. (ELISA, ELISPOT, FC)
  5. Doern A, Cao X, Sereno A, Reyes CL, Altshuler A, Huang F, et al. Characterization of inhibitory anti-insulin-like growth factor receptor antibodies with different epitope specificity and ligand-blocking properties: implications for mechanism of action in vivo. J Biol Chem. 2009;284:10254-67. (ELISA, WB)
  6. Vandenberg CJ, Josefsson EC, Campbell KJ, James C, Lawlor KE, Kile BT, et al. Loss of Bak enhances lymphocytosis but does not ameliorate thrombocytopaenia in BCL-2 transgenic mice. Cell Death Differ. 2014;21:676-84. (ELISA, ELISPOT)
  7. Dunand CJ, Leon PE, Kaur K, Tan GS, Zheng N, Andrews S, et al. Preexisting human antibodies neutralize recently emerged H7N9 influenza strains. J Clin Invest. 2015;125:1255-68. (ELISA, ELISPOT)
  8. Ju ZL, Shi GY, Zuo JX, Zhang JW, Sun J. Unexpected development of autoimmunity in BAFF-R-mutant MRL-lpr mice. Immunology. 2006;120:281-9. (ELISPOT, FC)
  9. Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, et al. Follicular dendritic cell (FDC) precursors in primary lymphoid tissues. J Immunol. 1998;160:1078-84. (FC, IHC-FS)
  10. Brakebusch C, Fillatreau S, Potocnik AJ, Bungartz G, Wilhelm P, Svensson M, et al. β1 integrin is not essential for hematopoiesis but is necessary for the T cell-dependent IgM antibody response. Immunity. 2002;16:465-77. (FC, IHC-FS)
  11. Moura IC, Arcos-Fajardo M, Sadaka C, Leroy V, Benhamou M, Novak J, et al. Glycosylation and size of IgA1 are essential for interaction with mesangial transferrin receptor in IgA nephropathy. J Am Soc Nephrol. 2004;15:622-34. (FC, ICC)
  12. Tsuneto M, Yamazaki H, Yoshino M, Yamada T, Hayashi S. Ascorbic acid promotes osteoclastogenesis from embryonic stem cells. Biochem Biophys Res Commun. 2005;335:1239-46. (FC, Sep)
  13. Zotos D, Coquet JM, Zhang Y, Light A, D’Costa K, Kallies A, et al. IL-21 regulates germinal center B cell differentiation and proliferation through a B cell-intrinsic mechanism. J Exp Med. 2010;207:365-78. (FC, IHC-FS)
  14. Thorum SC, Comstock SS, Hester SN, Shunk JM, Monaco MH, Donovan SM. Impact of diet on development of bronchial-associated immunity in the neonatal piglet. Vet Immunol Immunopathol. 2013;151:63-72. (FC)
  15. Jung S, Schickel J, Kern A, Knapp A, Eftekhari P, Da Silva S, et al. Chronic bacterial infection activates autoreactive B cells and induces isotype switching and autoantigen-driven mutations. Eur J Immunol. 2016;46:131-46. (FC)
  16. Nosanchuk JD, Valadon P, Feldmesser M, Casadevall A. Melanization of Cryptococcus neoformans in murine infection. Mol Cell Biol. 1999;19:745-50. (IHC-FS, IHC-PS)
  17. Zannettino AC, Holding CA, Diamond P, Atkins GJ, Kostakis P, Farrugia A, et al. Osteoprotegerin (OPG) is localized to the Weibel-Palade bodies of human vascular endothelial cells and is physically associated with von Willebrand factor. J Cell Physiol. 2005;204:714-23. (IHC-FS, ICC)
  18. Ji F, Liu Z, Cao J, Li N, Liu Z, Zuo J, et al. B cell response is required for granuloma formation in the early infection of Schistosoma japonicum. PLoS One. 2008;3(3):e1724. (IHC-FS, IHC-PS)
  19. Piersma B, de Rond S, Werker PM, Boo S, Hinz B, van Beuge MM, et al. YAP1 is a driver of myofibroblast differentiation in normal and diseased fibroblasts. Am J Pathol. 2015;185:3326-37. (IHC-FS, ICC)
  20. Ren B, Li J, Genschmer K, Hollingshead SK, Briles DE. The absence of PspA or presence of antibody to PspA facilitates the complement-dependent phagocytosis of pneumococci in vitro. Clin Vaccine Immunol. 2012;19:1574-82. (WB)
  21. El Karoui K, Viau A, Dellis O, Bagattin A, Nguyen C, Baron W, et al. Endoplasmic reticulum stress drives proteinuria-induced kidney lesions via Lipocalin 2. Nat Commun. 2016;7:10330. (IHC-PS)
  22. Piccioli D, Tavarini S, Borgogni E, Steri V, Nuti S, Sammicheli C, et al. Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets. Blood. 2007;109:5371-9. (Multiplex)
  23. Timmerman P, Barderas R, Desmet J, Altschuh D, Shochat S, Hollestelle MJ, et al. A combinatorial approach for the design of complementarity-determining region-derived peptidomimetics with in vitro anti-tumoral activity. J Biol Chem. 2009;284:34126-34. (Microarray)
  24. Irie-Maezono R, Tsuyama S. Immunohistochemical analysis of the acid secretion potency in gastric parietal cells. CellBio. 2013;2:179-85. (Block)

 

 

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