Bcl6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) 慢病毒載體 | 貨號LV808039

產品介紹: Bcl6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) 慢病毒表現載體。BCL6表達將由具有GFP報告基因的CMV啟動子驅動。 插入物的兩側是並且可以使用NheI和BamHI切除，只要插入物不含任何內部NheI或BamHI位點即可。Bcl6基因 (Accession Number NM_001130845) 架構於pLenti-GIII-CMV-GFP-2A-Puro表現載體，由CMV啟動子驅動， 具GFP Reporter。可直接以非病毒質粒進行轉染用於即時表達，或包裝成慢病毒顆粒用於高效轉導和穩定整合表達。病毒包裝系統建議使用ABMGOOD提供之轉染試劑 Cat# G074, Lentifectin™ 及優化之病毒包裝系列LV003。慢病毒包裝系列產品包括Lentivirus慢病毒低速純化(純化) / Lentivirus 慢病毒高濃度純化(純化) 並以 Lentivirus病毒RT-QPCR定量(定量) 純化後之病毒力價。

Description	The BCL6 expression will be driven by a CMV promoter, with a GFP reporter. Inserts are flanked by and can be excised using NheI and BamHI as long as inserts do not contain any internal NheI or BamHI sites.
Procedure Overview
Product Application	·  Direct non-viral plasmid transfection for immediate expression ·  Package into Lentiviral particles for high efficiency transduction and stably integrated expression
Gene Insert	Bcl6
Accession Number	NM_0011308451,2,3,4,5
Vector	pLenti-GIII-CMV-GFP-2A-Puro
Vector Size	8829bp
Insert Size	2121 bp
Species	Human
Tags	GFP
Promoter	CMV
Selection Marker	Bacterial: Kanamycin. Mammalian: Puromycin
Format	Lentiviral Plasmid
Viral Packaging	abm recommends using Cat# G074, Lentifectin™ in conjunction with one of the following packaging mixes. The suggested packaging mix for this vector is Cat# LV003, 2nd Generation Packaging Mix. Alternatively, you can also use Cat# LV053, 3rd Generation Packaging Mix
Appearance	Clear Liquid
Storage Buffer	10mM Tris-HCI, 1mM EDTA, pH8.0
Stability	1 year when stored at -20°C or lower in a non-frost free freezer.

慢病毒技術之領導者abm，開發了一套全面的人類、小鼠和大鼠基因文庫，Clone到慢病毒載體或製成即用型慢病毒，可用於操縱感興趣基因於宿主細胞表達。為了促進我們的慢病毒載體的應用和多功能性，ABM慢病毒表達系統具有許多組成型啟動子，標籤序列和報導基因之組合。