永生化人類多巴胺能神經元前體細胞 | Immortalized LUHMES- v-myc genes 貨號T0284
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Abmgood不朽化系列商品 (immortalized cell)
永生化人類多巴胺能神經元前體細胞（LUHMES）- v-myc genes (貨號T0284)
Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES) Homo sapiens
永生化的Dopaminergic Neuronal Precursor Cells人類多巴胺能神經元前體細胞，也稱為Lund Human Mesencephalic隆德人中腦（LUHMES）細胞，是四環素控制的，v-myc過表達的人中腦衍生的細胞系MESC2.10的subclone。 該細胞是獨特的，因為它可以在適當的生長條件下分化為獲得dopaminergic neuron-like多巴胺能神經元樣表型phenotype。 LUHMES在分化後表達功能性dopamine transporter多巴胺轉運蛋白（DAT），vesicular monoamine transporter囊泡單胺轉運蛋白（VMAT-2），tyrosine hydroxylase酪氨酸羥化酶（TH）和β-III tubulin微管蛋白的神經元形式。 除了保留多巴胺能神經元特異性標記DAT, VMAT-2, TH, α-SYN, β-III tubulin之外，LUHMES還表現出電生理學特性，因此使得該細胞系成為neurodevelopmental神經發育研究，疾病建模和neuropharmacology神經藥理學的有意思之神經元細胞Model。
| Organism | Homo sapiens |
| Source Organ | 8-week-old fetal human ventral mesencephalon |
| Donor Age | 8 week |
| Donor Gender | undetermined |
| BioSafety Level | II |
| Growth Properties | Adherent |
| Morphology | Flattened|Dendritic processes |
| Recommended Seeding Density | 50,000 – 100,000 cells/cm2; cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5 |
| Population Doubling | 30 – 40 hours |
| Immortalization Method | Conditional immortalization by tetracyclin-controlled transduction with retrovirus carrying v-myc genes |
| Description | The Immortalized Human Dopaminergic Neuronal Precursor Cells, also known as the Lund Human Mesencephalic (LUHMES) cell line, is a subclone of the tetracycline-controlled, v-myc-overexpressing human mesencephalic-derived cell line MESC2.10. This cell line is unique in that it can be differentiated to acquire a dopaminergic neuron-like phenotype under appropriate growth conditions. LUHMES expresses functional dopamine transporter (DAT), vesicular monoamine transporter (VMAT-2), tyrosine hydroxylase (TH) and the neuronal form of β-III tubulin after differentiation. In addition to retaining dopaminergic neuronal-specific markers, LUHMES also exhibit electrophysiological properties, thus making this cell line a valuable neuronal model for neurodevelopmental studies, disease modelling and neuropharmacology. |
| Applications | For Research Use Only |
| Markers | DAT, VMAT-2, TH, α-SYN, β-III tubulin |
永生化人類多巴胺能神經元前體細胞- v-myc genes 細胞特性: Adherent | 不杇方法: tetracyclin-controlled transduction with retrovirus carrying v-myc genes | 來源8-week-old fetal human ventral mesencephalon。細胞型態: Flattened|Dendritic processes。細胞培養液更換大約1週2-3次；細胞繼代為胰蛋白酶溶液0.25% trypsin(TM050)。細胞建議之生長密度為50,000 – 100,000 cells/cm2,; Population Doubling30 – 40 hours細胞的活力約為40-50％。 解凍後，細胞需要4-5天才能恢復培養; 推薦的Seeding比例是1：4至1：5。 細胞培養皿pre-coated需預包覆50μg/ ml poly-L-ornithine (PLO)（TM062）和1μg/ ml fibronectin（EMD Millipore; Cat.FC010）/H 2 O, 細胞37℃下培養至少3小時。該細胞系的基礎培養基是Advanced DMEM / F12（Gibco; 12634010）。complete growth medium完整的生長培養基製備，將下列組分添加到基礎培養基中：1x N2 supplement（ThermoFisher Scientific），2mM L-glutamine（G275）和40ng / ml recombinant bFGF（Z101455）。二氧化碳（CO2）：5％，溫度：37.0℃。每2-3天更換一次complete media。不要讓complete media顏色變成橙黃色。
當受到壓力時，細胞會形成clumps圓團而不是monolayer單層。建議細胞生長至50-60％的細胞密度時，繼代培養。為了繼代培養細胞，使用0.25％胰蛋白酶-EDTA（TM051）。加入胰蛋白酶後，將細胞在室溫下孵育2-3分鐘並攪拌培養皿直至90％的細胞脫落。使用胰蛋白酶中和溶液（Lonza，貨號CC-5002）立即中和胰蛋白酶。添加胰蛋白酶中和溶液兩倍的胰蛋白酶使用量。 200×g離心2-3分鐘。吸出上清液，並在完全培養基中resuspend細胞。Plate細胞到預熱的PLO-fibronectin-coated vessels在37℃。如果細胞出現壓力，請避免繼代培養。注意：如果在周末（或超過2天）離開細胞，請確保做到高比例之細胞密度培養（1：4至1：5）。
為了將細胞分化成神經元，在細胞生長至40-50％的密度後將培養基更換為分化培養基。為了製備完全的分化培養基，將以下組分添加到基礎的Advanced DMEM / F12（Gibco; 12634010）中：1×N2補充物，2mM L-谷氨酰胺，1mM dbcAMP，1μg/ ml四環素和2ng / ml重組人GDNF（Z101055）。在測試神經元特異性標記前，讓細胞在分化培養基中靜置4-6天。
Grow the cells in culture vessel pre-coated with 50 μg/ml poly-L-ornithine (PLO) (TM062) and 1 μg/ml fibronectin (EMD Millipore; Cat. FC010) in H2O for at least 3 hours at 37°C. Do not grow these cells in culture vessels with surface areas equal to less than 12.5 cm2. These cells do not grow in 6-well, 24-well, 48-well, or 96-well plates.
The base medium for this cell line is Advanced DMEM/F12 (Gibco;12634010). To make the complete growth medium, add the following components to the base medium: N2 supplement (ThermoFisher Scientific) to a final concentration of 1X, L-glutamine (G275) to a final concentration of 2 mM, and Recombinant Human FGF2 (Z101455) to a final concentration of 40 ng/ml.
Change media every 2-3 days. Do not let media colour change to orange-yellow.
The cells will form round clumps instead of a monolayer when stressed. It is recommended to subculture when cells are grown to a cell density of 50-60%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
To subculture the cells, use 0.25% Trypsin-EDTA (TM051). After adding trypsin, incubate the cells at room temperature for 2-3 minutes and agitate the culture vessel until 90% of the cells have detached. Immediately neutralize the trypsin using Trypsin Neutralizing Solution (Lonza, Cat. CC-5002). Add Trypsin Neutralizing Solution twice the volume of trypsin used. Centrifuge at 200x g for 2-3 minutes. Aspirate supernatant and resuspend cells in complete media. Plate cells into pre-warmed PLO-fibronectin-coated vessels at 37.0°C. Avoid subculturing if the cells appear stressed. Note: If leaving the cells over the weekend (or more than 2 days), make sure to do a high split ratio (1:4 to 1:5).
To differentiate the cells into neurons, change the medium to differentiation medium after the cells have grown to a density of 40-50%.
To make complete differentiation medium, add the following components to Advanced DMEM/F12 (Gibco;12634010): N2 supplement to a final concentration of 1X, L-glutamine (G275) to a final concentration of 2 mM, dbcAMP to a final concentration of 1 mM, tetracycline to a final concentration of 1 μg/ml, and Recombinant Human GDNF (Z101055) to a final concentration of 2 ng/ml. Allow the cells to grow in differentiation medium for 4-6 days before testing for neuron specific markers.
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Abmgood不朽化系列商品 (immortalized reagenet) Ready-to-use
Abmgood公司於人類與老鼠不朽之細胞株上，具有幾十年的實驗經驗；提供一系列不朽 (immortalized)化之細胞株、及不朽化之試劑商品。ready-to-use定量好之retrovirus、lentiviru、 adenovirus，可直接加於細胞中進行感染表現；病毒液包括有SV40重組病毒、EBV重組病毒、hTERT病毒液、Myc基因病毒液、p53基因病毒液、Rb基因病毒液、Ras基因病毒液。提供106及109兩種包裝力價之病毒液；亦提供一系列病毒力價濃縮與定量試劑商品。
細胞不朽化的工具中，SV40 T antigen是最簡單且最常用來造成不朽化細胞生成的方法。多數的病毒基因和抑癌基因（p53、Rb等）的不活化相關，會誘導細胞處於replicative senescence的階段。目前也有研究顯示被感染的細胞，SV40 T anbtigen會誘導端粒酶（Telomerase）的活性。與端粒（telomere）長度相關的Telomerase Reverse Transcriptase protein (TERT)是一種不朽細胞的研究應用。平常細胞中，hTERT是處於不活化態，必要時會進行活化保持telomere的長度，避免細胞發生replicative senescence的現象。某些細胞hTERT 過度表現（over-expression）對於誘發細胞不朽化是無效的（如初代上皮細胞），反而會造成細胞死亡；同時表現hTERT catalytic subunit以及p53或RB siRNA在人類初代卵巢上皮細胞會造成細胞不朽化。過度表現Ras或Myc T58A mutants也有初代細胞不朽化的現象產生。
Abmgood不朽化系列商品 (immortalized cell)
根據細胞類型，Primary cells初代細胞在體外具有小於10-50次傳代的有限壽命。 不朽化細胞株能夠延長增殖，並且具有與親代組織相似或相同的基因型和表現型。
哺乳動物細胞的不朽化Immortalized策略
存在用於在培養物中不朽化哺乳動物細胞的幾種方法。 Abmgood多年研發經驗，開發出了一系列Immortalized商品；包括即用型逆轉錄病毒，慢病毒和腺病毒載體。
| Gene Overexpressed | Viruses Offered | Application |
| hTERT | Adenovirus, Lentivirus, Retrovirus |
可靠和常用。 可能在一些細胞類型（例如上皮細胞）中引起細胞死亡 |
| SV40 T Antigen | Adenovirus, Lentivirus, Retrovirus |
可靠和常用。 通常用於不朽化上皮細胞。 |
| EBV genes | Epstein-Barr Virus | 不朽化B淋巴細胞，有時是T淋巴細胞。 |
| HPV E6-E7 | Lentivirus | 不朽化角化細胞。 |
| HOXB8, HOXA9, HOXA10 | Lentivirus | 不朽化造血細胞，包括巨噬細胞，造血祖細胞和骨髓祖細胞。 |
| Adenoviral E1A-E1B | Lentivirus | 不朽化大範圍的初級囓齒動物細胞，包括羊水細胞，原發性上皮細胞，成纖維細胞和原代腎細胞。 |
| p53, Rb siRNA | Lentivirus | 與hTERT共表達以更好地使上皮細胞不朽化。 |
| Bmi1 | Lentivirus | 不朽化上皮細胞。 |
| CDK4 | Lentivirus | 與hTERT共同表達更真實的細胞模型。 |
| Myc T58A (c-Myc), Ras V12 (H-Ras) | Lentivirus | 不朽化各種細胞，包括神經幹細胞，初代腎細胞，成紅細胞和各種上皮細胞（原發性前列腺，乳腺，氣道等）。 |
Decomplement加熱非活化FBS：
1.讓FBS瓶在4°C下完全解凍過夜。
2.將FBS瓶置於56°C的水浴中30分鐘。
Decomplemented FBS可以在-20°C下長期保存。避免頻繁的凍融循環。每2-3天更換一次完整的媒體。不要讓介質顏色變為黃色。
Thawing細胞解凍方法：(每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
1.在37℃水浴中解凍小瓶，直到只有一小塊冰塊。(DMSO對細胞有毒性，over-thaw過度解凍下可能造Stress)
2.將細胞轉移到含有5ml pre-warmed complete media 之15ml管中。
3.將細胞以290xg離心5分鐘。
4.小心丟棄上清液，不要打擾細胞沉澱，輕輕地上下移液，將細胞重新懸浮在1ml完全培養基中。
5.將細胞培養在50,000 – 100,000 cells/cm2。 cells will have viability of around 40-50%. Post thawing, it will need 4-5 days for cells to recover in culture; Recommended split ratio is 1:4 to 1:5
DMSO在細胞凍存中的應用
二甲基亞碸是一種重要的滲透型細胞保護劑。在深低溫（零下200度）保存細胞時凍存過程中，為防止細胞內液冰晶形成、滲透壓改變、細胞結構紊亂等導致的損傷，有必要使用含有DMSO冷凍保護劑。DMSO能夠快速穿透細胞膜進入細胞中，降低冰點、延緩凍存過程，同時提高細胞內離子濃度，減少細胞內冰晶的形成，從而減少細胞損傷。深低溫時二甲基亞碸的細胞毒性受到抑制。復蘇時動作要快，儘快洗掉二甲基亞碸，否則會造成對細胞嚴重的毒性。二甲基亞碸（DMSO）是目前最好的細胞凍存保護劑，但也是一種以細胞毒性很大的化學試驗劑。研究結果表明，培養液中DMSO濃度為10%時，細胞生長抑制率近100%；1‰濃度時抑制率為35%，即使是0.04‰的濃度，DMSO對細胞的生長也有不利的影響。
Subculture細胞繼代方法: (每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
1.當細胞70-90％ confluency時，建議繼代培養。
2. Aspirate old media。懸浮液中的一些細胞仍然是活細胞。或者，也可以收集含有懸浮細胞的上清液並離心（Skip to Step 6 in protocol）。
3.加入1：1比例的1X無菌PBS和0.25％胰蛋白酶-EDTA（TM051）。
4.在室溫下孵育細胞3-5分鐘並攪拌培養容器直至90％的細胞脫落。
5. 中和胰蛋白酶:添加等量之trypsin + PBS。
6.收集細胞並以290xg離心5分鐘。
7.丟棄上清液，輕輕地上下移液，將細胞重新懸浮在完全培養基中。
8.建議split ratio比不超過1：3。
Freezing細胞凍結：(每種細胞建議條件可能有許不同，依照原廠建議操作，以下為通用方式) 操作方式下載
Complete growth medium with decomplemented FBS to a final concentration of 50% from PAN Biotech (P40-37500) or from Nucleus Biologics (FBS1824-001) and 5% DMSO.
儲存溫度：液氮氣相。重要提示：此細胞係對FBS批次非常敏感。我們強烈建議最終用戶使用從PAN biotech或Nucleus Biologics 之FBS。
慢病毒包裝系列產品Lentivirus慢病毒低速純化(純化) | Lentivirus 慢病毒高濃度純化(純化) | Lentivirus病毒RT-QPCR定量(定量)
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| T0697 | Immortalized Human Cranial Suture Progenitor Cells (iSuPs) |
| T0723 | Immortalized Human Foreskin Keratinocytes (HFK-16E6E7) |
| T0727 | Immortalized Human Prostate Stromal Cells (PM151T) – hTERT |
| T0752 | Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) |
| T0764 | Immortalized Human Endometriotic Cell Line (12Z) |
| T0841 | Immortalized Human Dental Pulp Stem Cells (TP-023) |
| T0842 | Immortalized Human Dental Pulp Stem Cells (TP-037) |
| T1013 | Immortalized Human Diploid Fibroblasts – hTERT |
| T1015 | Immortalized Human Lung Fibroblast – hTERT |
| T9435 | Immortalized Human Cancer Associated Fibroblast Cells – SV40 |
| T3000 | Acan CRISPR Knockout RCS Cell Line (Rat) |
| T3002 | Aquaporin-4 Stable 293 Cell Line |
| T3005 | MuSK Stable HEK293 Cell Line |
| T3006 | Luciferase Stable PC3 Cell Line |
| T3008 | Luciferase (Luc2) Stable TRAMP Cell Line |
| T3009 | Luciferase Stable U87MG/ΔEGFR Cell Line |
| T3013 | Luciferase Stable WM-266-4 Cell Line |
| T3015 | NF-kB Luciferase Stable RAW264.7 Cell Line |
| T3016 | HEK293-CymR-Puro Stable Cell Line |
| T3017 | A2058-CymR Stable Cell Line |
| T3018 | SKMEL5-CymR Stable Cell Line |
| T3021 | GRPR-Luciferase Stable PC3 Cell Line |
| T3024 | Bax Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3025 | Bak Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3026 | Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3027 | Atg5 Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3028 | Atg7 Knockout Immortalized Mouse Kidney Epithelial Cell Line |
| T3030 | Nav2.1/β1/Contactin Stable Chinese Hamster Lung Cell Line |
| T3031 | PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line |
| T3032 | Dual Notch1-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1) Cell Line |
| T3033 | Dual Notch1(Variant)-YFP and Inducible Delta-mCherry Reporter CHO-K1 (hN1G4<sup>esn</sup>) Cell Line |
| T3034 | TLR3 Knockout Immortalized Mouse Dendritic Cell Line |
| T3035 | TLR9 Knockout Immortalized Mouse Dendritic Cell Line |
| T3036 | Type I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line |
| T3037 | Ryanodine Receptor 1 Stable HEK293 Cell Line |
| T3038 | KCC2 Stable HEK293 Cell Line |
| T3039 | Stable HEK293 Expressing Mutant UQCRB-MT1 Cell Line |
| T3040 | c-Kit/D816V Stable Ba/F3 Cell Line |
| T3041 | c-Kit/WT Stable Ba/F3 (with GNNK- isoform) Cell Line |
| T3042 | c-Kit/WT Stable Ba/F3 (with GNNK+ isoform) Cell Line |
| T3043 | c-Kit/Y568F Stable Ba/F3 Cell Line |
| T3044 | c-Kit/Y570F Stable Ba/F3 Cell Line |
| T3045 | c-Kit Y568F/Y570F Stable Ba/F3 Cell Line |
| T3046 | c-Kit/Y703F Stable Ba/F3 Cell Line |
| T3047 | c-Kit/Y936F Stable Ba/F3 Cell Line |
| T3048 | c-Kit Y703F/Y936F Stable Ba/F3 Cell Line |
| T3049 | c-Kit/N705AStable Ba/F3 Cell Line |
| T3050 | c-Kit/N938A Stable Ba/F3 Cell Line |
| T3051 | c-Kit N705A/N938A Stable Ba/F3 Cell Line |
| T3052 | c-Kit/D816H Stable Ba/F3 Cell Line |
| T3053 | c-Kit/D816F Stable Ba/F3 Cell Line |
| T3054 | c-Kit/D816YStable Ba/F3 Cell Line |
| T3055 | c-Kit/V560D Stable Ba/F3 Cell Line |
| T3056 | c-Kit/K642E Stable Ba/F3 Cell Line |
| T3057 | c-Kit/Y721F Stable Ba/F3 Cell Line |
| T3058 | c-Kit/Y823F Stable Ba/F3 Cell Line |
| T3059 | TLR2 Stable HEK293 Cell Line |
| T3060 | PGC-1α/ERRα Stable 293T Cell Line |
| T3061 | TNK1 Stable Ba/F3 Cell Line |
| T3062 | ALK Stable Ba/F3 Cell Line |
| T3063 | LTK Stable Ba/F3 Cell Line |
| T3064 | AXL Stable Ba/F3 Cell Line |
| T3065 | MERTK Stable Ba/F3 Cell Line |
| T3066 | TYRO3 Stable Ba/F3 Cell Line |
| T3067 | EPHA1 Stable Ba/F3 Cell Line |
| T3068 | EPHA5 Stable Ba/F3 Cell Line |
| T3069 | EPHA7 Stable Ba/F3 Cell Line |
| T3070 | EPHA8 Stable Ba/F3 Cell Line |
| T3071 | FGFR2 Stable Ba/F3 Cell Line |
| T3072 | FGFR3 Stable Ba/F3 Cell Line |
| T3073 | IGF1R Stable Ba/F3 Cell Line |
| T3074 | INSR Stable Ba/F3 Cell Line |
| T3075 | JAK1 Stable Ba/F3 Cell Line |
| T3076 | JAK2 Stable Ba/F3 Cell Line |
| T3077 | JAK3 Stable Ba/F3 Cell Line |
| T3078 | MET Stable Ba/F3 Cell Line |
| T3079 | MST1R Stable Ba/F3 Cell Line |
| T3080 | FLT3 Stable Ba/F3 Cell Line |
| T3081 | KIT Stable Ba/F3 Cell Line |
| T3082 | PDGFRA Stable Ba/F3 Cell Line |
| T3083 | PDGFRB Stable Ba/F3 Cell Line |
| T3084 | RET Stable Ba/F3 Cell Line |
| T3085 | ROS1 Stable Ba/F3 Cell Line |
| T3086 | BLK Stable Ba/F3 Cell Line |
| T3087 | FGR Stable Ba/F3 Cell Line |
| T3088 | FYN Stable Ba/F3 Cell Line |
| T3089 | HCK Stable Ba/F3 Cell Line |
| T3090 | LCK Stable Ba/F3 Cell Line |
| T3091 | SRC Stable Ba/F3 Cell Line |
| T3092 | YES1 Stable Ba/F3 Cell Line |
| T3093 | SYK Stable Ba/F3 Cell Line |
| T3094 | NTRK1 Stable Ba/F3 Cell Line |
| T3095 | NTRK2 Stable Ba/F3 Cell Line |
| T3096 | NTRK3 Stable Ba/F3 Cell Line |
| T3097 | FLT4 Stable Ba/F3 Cell Line |
| T3098 | ERBB2 Stable Ba/F3 Cell Line |
| T3099 | EPHA4 Stable Ba/F3 Cell Line |
| T3100 | ABL1 Stable Ba/F3 Cell Line |
| T3101 | ABL2 Stable Ba/F3 Cell Line |
| T3102 | AZ-AHR Stable HepG2 Luciferase Reporter Cell Line |
| T3103 | AZ-GR Stable HeLa Luciferase Reporter Cell Line |
| T3104 | AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line |
| T3105 | BMP Responsive Immortalized Reporter (BRITER) Cell Line |
| T3106 | EGFR Stable Ba/F3 Cell Line |
| T3107 | ERBB4 Stable Ba/F3 Cell Line |
| T3108 | EPHA2 Stable Ba/F3 Cell Line |
| T3109 | EPHB1 Stable Ba/F3 Cell Line |
| T3110 | EPHB2 Stable Ba/F3 Cell Line |
| T3111 | TYK2 Stable Ba/F3 Cell Line |
| T3112 | EPHB4 Stable Ba/F3 Cell Line |
| T3113 | FER Stable Ba/F3 Cell Line |
| T3115 | LYN Stable Ba/F3 Cell Line |
| T3116 | ZAP70 Stable Ba/F3 Cell Line |
| T3117 | TIE1 Stable Ba/F3 Cell Line |
| T3118 | KDR Stable Ba/F3 Cell Line (IL-3 Independent) |
| T3119 | TNK2 Stable Ba/F3 Cell Line |
| T3120 | CSK Stable Ba/F3 Cell Line |
| T3121 | MATK Stable Ba/F3 Cell Line |
| T3122 | DDR1 Stable Ba/F3 Cell Line |
| T3123 | DDR2 Stable Ba/F3 Cell Line |
| T3124 | PTK2 Stable Ba/F3 Cell Line |
| T3126 | EPHA10 Stable Ba/F3 Cell Line |
| T3127 | EPHA3 Stable Ba/F3 Cell Line |
| T3128 | EPHA6 Stable Ba/F3 Cell Line |
| T3129 | EPHB3 Stable Ba/F3 Cell Line |
| T3131 | PTK2B Stable Ba/F3 Cell Line |
| T3132 | FGFR4 Stable Ba/F3 Cell Line |
| T3133 | INSRR Stable Ba/F3 Cell Line |
| T3134 | AATK Stable Ba/F3 Cell Line |
| T3136 | LMTK3 Stable Ba/F3 Cell Line |
| T3137 | MuSK Stable Ba/F3 Cell Line |
| T3138 | CSF1R Stable Ba/F3 Cell Line |
| T3139 | PTK7 Stable Ba/F3 Cell Line |
| T3140 | ROR1 Stable Ba/F3 Cell Line |
| T3141 | ROR2 Stable Ba/F3 Cell Line |
| T3142 | RYK Stable Ba/F3 Cell Line |
| T3143 | FRK Stable Ba/F3 Cell Line |
| T3145 | SRMS Stable Ba/F3 Cell Line |
| T3146 | BMX Stable Ba/F3 Cell Line |
| T3147 | BTK Stable Ba/F3 Cell Line |
| T3148 | ITK Stable Ba/F3 Cell Line |
| T3149 | TEC Stable Ba/F3 Cell Line |
| T3150 | TXK Stable Ba/F3 Cell Line |
| T3151 | TEK2 Stable Ba/F3 Cell Line |
| T3152 | FLT1 Stable Ba/F3 Cell Line |
| T3153 | STYK1 Stable Ba/F3 Cell Line |
| T3154 | SPINK1 Stable AsPC-1 Cell Line |
| T3155 | hSSAO/VAP-1 A7r5 Stable Cell Line |
| T3156 | Mouse R-spondin1 Stable 293T Cell Line |
| T3157 | Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line |
| T3158 | TrkA Stable T17 Cell Line |
| T3159 | TrkB Stable T48 Cell Line |
| T3160 | Luc-Akt-PH Stable MCF7 Cell Line |
| T3161 | Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line |
| T3162 | HEK293S-TetR Stably Expressing TP Cell Line |
| T3163 | HEK293S-TetR GnTI- Stably Expressing TP Cell Line |
| T3164 | HEK293S-TetR Stably Expressing A160T Cell Line |
| T3165 | HEK293S-TetR GnTI- Stably Expressing A160T Cell Line |
| T3166 | Cx26 Stable BxPC-3 Cell Line |
| T3167 | E-cadherin Stable MIA PaCa-2 Cell Line |
| T3168 | BMP9 Stable HEK293 Cell Line |
| T3169 | 92 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line |
| T3170 | 88 Tag Stable Y-LIZ DNA Polymerase-β Knockout Mouse Embryonic Fibroblast Cell Line |
| T3171 | SIRT1 Wiltype Stable RKO Cell Line |
| T3172 | SIRT1 Knockout Stable RKO Cell Line |
| T3173 | SIRT1 Wildtype Stable DLD-1 Cell Line |
| T3174 | SIRT1 Knockout Stable DLD-1 Cell Line |
| T3175 | SIRT1 Wildtype Stable 293T Cell Line |
| T3176 | SIRT1 Knockout Stable 293T Cell Line |
| T3177 | PZ-TR Stable HepG2 Luciferase Reporter Cell Line |
| T3178 | Ad5 pTP Stable 293 (293pTP) Cell Line |
| T3181 | p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line |
| T3182 | Podoplanin Stably Expressing CHO Cell Line |
| T3183 | Stable Retinal Pigment Epithelial Expressing Truncated hELG1-CFP Cell Line |
| T3184 | Stable EGFR and Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B1B2F3) Cell Line |
| T3185 | Stable Erbb2 Conditional Knockdown Mouse Skin Keratinocyte (B2F6) Cell Line |
| T3186 | Stable EGFR Conditional Knockdown Mouse Skin Keratinocyte (Egfr2) Cell Line |
| T3187 | Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line |
| T3188 | Stable Rat Expressing HA-tagged SCL26A9 Thyroid Epithelial (SLC26A9-FRT) Cell Line |
| T3189 | Stable VERO Overexpressing HER2 Cell Line |
| T3190 | Stable VERO Overexpressing HER3 Cell Line |
| T3191 | Stable VERO Overexpressing HER2 and HER3 Cell Line |
| T3192 | Stable VERO Overexpressing HER1 Cell Line |
| T3193 | Stable CHO Overexpressing HER2 Cell Line |
| T3194 | Stable VERO Overexpressing HER1 and HER2 Cell Line |
| T3195 | Stable HepG2 Expressing Murine ADH1 (VA-13) Cell Line |
| T3196 | Stable HA-Tagged hGALE Expressing CHO Cell Line |
| T3197 | Stable G90E Mutated hGALE Expressing CHO Cell Line |
| T3198 | Stable V94M Mutated hGALE Expressing CHO Cell Line |
| T3199 | Stable C307Y Mutated hGALE Expressing CHO Cell Line |
| T3203 | CRE Expressing 293 Cell Line |
| T3207 | ICP47 Stable Raji Cell Line |
| T3208 | ICP47 Stable Mel JuSo Cell Line |
| T3209 | HLA-DQ8 Expressing Stable K562 Cell Line |
| T3210 | Mutant AKT1 Stable Proteus Syndrome Derived Cell Line (F6B) |
| T3217 | GFP Stably Expressing Schwann cells |
| T3218 | huGRPr Expessing Stable Ace-1 Cell Line |
| T3219 | Apolipoprotein A-1 Expressing Stable MC38 Cell Line (MC38 Apo) |
| T3220 | Apolipoprotein A-1 and P144 Over-expressing Stable MC38 Cell Line (MC38 ApoLinkerP144) |
| T3222 | BRCA1 L1780P Mutant Stable HCC1937 Cell Line |
| T3223 | BRCA1 L1780P Mutant Stable HCC1395 TNBC Cell Line |
| T3224 | NDUFS2 Stable Knockout HEK293 Cell Line (HEK293ΔNDUFS2) |
| T3229 | HEK293-T Cells Stably Expressing MLL-AF6 (MA6) |
| T3230 | HEK293-T Cells Stably Expressing AF6-MLL (A6M) |
| T3231 | HEK293-T Cells Stably Expressing MLL-AF6/AF6-MLL (CO1) |
| T3232 | HEK293-T Cells Stably Expressing exMLL-AF6 (exMA6) |
| T3233 | HEK293-T Cells Stably Expressing AF6-shMLL (A6shM) |
| T3234 | HEK293-T Cells Stably Expressing exMLL-AF6/AF6-shMLL (CO2) |
| T3235 | A549 Cells Stably Expressing hACE2 Receptor and TMPRSS2 (A549-AT) |
| T3236 | U2OS Stably Expressing NONO-APEX2 Cell Line (U2OS:NONO-APEX2-HA) |
| T3241 | ts-46 Stable Knockout HEK293 Cell Line (ts-46 293KO) |
| T3242 | ts-101 Stable Knockout HEK293 Cell Line (ts-101 293KO) |
| T3243 | Human Progerin Expressing 3T3-L1 Stable Cell Line (3T3L1-PG) |