Scrambled sgRNA CRISPR Lentivirus | 貨號K018

產品介紹:  Abmgood提供sgRNA-CRISPR Lentivirus, 超過90％的構建體將在20-90％的細胞中實現完全敲除。 大多數構建體> 50％  transduced轉導和selected cells。 越來越多的證據表明，sgRNA比shRNA和siRNA敲除機率高。如果設計用於Cas9核酸酶的sgRNA構建體沒有合理的敲除，將免費設計另一組3個構建體。需確保您盡可能地優化了您的實驗，包括（如適用）增加您的MOI（最多10次）和感染持續時間（最多72小時），並在進行clone screening克隆篩選。

roduct Application	·  Direct non-viral plasmid transfection for immediate expression ·  Package into Lentiviral particles for high efficiency transduction and stably integrated expression
Species	N/A
Vector	pLenti-U6-sgRNA-PGK-Neo
Target Sequence	GCACTCACATCGCTACATCA
Format	Plasmid
Appearance	Liquid
Storage	-20°C or below
Shelf Life	1 year (when at -20°C or below in a non-frost free freezer)
Shipping	Shipped at ambient temperature
Additional Materials	– CRISPR Genomic Cleavage Detection Kit (G932)
Quality Control	Restriction Enzyme Digest and Sequencing
Caution	This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Note	abm‘s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).
Disclaimer	1. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.
Species	N/A
Vector	pLenti-U6-sgRNA-PGK-Neo
Target Sequence	GCACTCACATCGCTACATCA
Format	Virus
Appearance	Liquid
Storage	Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Shelf Life	1 year (when at -80°C or below in a non-frost free freezer)
Shipping	Shipped with dry ice
Additional Materials	– CRISPR Genomic Cleavage Detection Kit (G932)
Quality Control	Restriction Enzyme Digest and Sequencing

HOT 慢病毒（Lentivirus）表現最佳化  改良式包裝系統 | 病毒純化與濃縮 | 病毒力價定量系列商品 提昇慢病毒感染力

慢病毒（Lentivirus）的結構可分成套膜（envelop）、蛋白質膜（capsid）、以及遺傳物質。Lentivirus感染宿主後，會將其基因嵌入宿主細胞使其表達。現為實驗室中常見的基因重組工具。lentivirus已可藉由　1. 帶有target gene的lentivector，加上　2. packaging system，構成無致病性的psuedovirus。

Lentivirus慢病毒低速30分純化套組(純化)| Lentivirus 慢病毒高濃度純化(純化) | Lentivirus病毒RT-QPCR定量(定量) | Lentivirus病毒定量快篩片(定量卡)