DNase I /脫氧核糖核酸酶I | 貨號G028    2000 U (1.0 ml)

產品介紹: 脫氧核糖核酸酶I（DNase I; EC 3.1.21.1）是催化單鏈/雙鏈DNA，染色質和RNA-DNA雜交體中磷酸二酯鍵裂解的非特異性內切核酸酶。 DNA酶I切割DNA以釋放二 – 和/或寡核苷酸（5′-磷酸化和3′-羥基化）終產物。 一個單位定義；為在37℃下在10分鐘內催化1μgDNA降解為四核苷酸或更小片段的DNA酶I的量。產品應用: 從蛋白製劑和RNA樣品中去除DNA、介詞切口翻譯、生隨機片段用於雙脫氧測序、體外轉錄後降解模板DNA、介導DNA酶I印跡。

• Removes DNA from protein preparations
• Mediates nick translation
• Generates random fragments for dideoxy sequencing
• Degrade template DNA following in vitro transcription
• Mediate DNase I foot-printing

DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA.  For RNAse-Free DNAse I, see Cat. No. E091.

RNaseOFF核糖核酸酶抑制劑 | 貨號G138 4000U

RNaseOFF核糖核酸酶抑制劑以高親和力特異性抑制常見的核糖核酸酶（RNase），包括RNA酶A，RNA酶B和RNA酶C。 該酶在PCR或RT-PCR中是有用的添加劑，因為它可以安全地保護RNA免受潛在的RNase污染，而不會抑制聚合酶活性。 這種強大的RNase抑制劑版本對高度氧化敏感的人類RNase抑制劑具有改善的抗氧化性能。 RNaseOFF核糖核酸酶抑制劑即使在非常低濃度的DTT（<1 mM）下也是穩定的，使其成為最終RNA保護的最佳選擇。

產品注意事項: 一個單位定義為將5ng RNA酶A的活性抑制50％所需的RNaseOFF核糖核酸酶抑制劑的量。在反應設置期間，始終將RNaseOFF保留在冰上。建議在消除RNase污染的條件下設置所有RNA相關/ RNA酶敏感型工作。 移液管尖端和管子應用0.1％DEPC處理。使用RNA時，強烈建議佩戴手套。

DNase I (RNAse-Free) | 貨號E091 200 U (100 μl)

abm提供了一系列核酸酶(nucleases)，可製備RNA和DNA，以便在下一代全基因組測序(whole genome sequencing)和文庫構建(library construction)中用於後續應用。DNase I脫氧核糖核酸酶I（Deoxyribonuclease I , DNase I； EC 3.1.21.1）是一種非特異性核酸內切酶(non-specific endonuclease)，可催化單/雙鏈DNA((single/double-stranded DNA)，染色質(chromatin)和RNA-DNA雜交體中磷酸二酯鍵(phosphodiester bonds)的裂解。 DNase I切割DNA以釋放二和/或寡核苷酸（5’-phosphorylated , 5′-磷酸化和3’-hydroxylated , 3′-羥基化）終產物。該產品可用於RNA應用。

SKU	E091
Unit quantity	200 U (100 μl)
Applications	• Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing
Concentration	2 U/μl
Note	One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA. This product is distributed for laboratory research only Caution: Not for diagnostic use. RNaseOFF核糖核酸酶抑制劑 | DNase I (RNAse-Free) | DNase I