DNase I (RNAse-Free)非特異性核酸內切酶 | ABMGOOD貨號E091
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abm提供了一系列核酸酶(nucleases)，可製備RNA和DNA，以便在下一代全基因組測序(whole genome sequencing)和文庫構建(library construction)中用於後續應用。DNase I脫氧核糖核酸酶I（Deoxyribonuclease I , DNase I； EC 3.1.21.1）是一種非特異性核酸內切酶(non-specific endonuclease)，可催化單/雙鏈DNA((single/double-stranded DNA)，染色質(chromatin)和RNA-DNA雜交體中磷酸二酯鍵(phosphodiester bonds)的裂解。 DNase I切割DNA以釋放二和/或寡核苷酸（5’-phosphorylated , 5′-磷酸化和3’-hydroxylated , 3′-羥基化）終產物。該產品可用於RNA應用。
RNaseOFF核糖核酸酶抑制劑 | DNase I (RNAse-Free) | DNase I
| SKU | E091 |
| Unit quantity | 200 U (100 μl) |
| Applications | • Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
| Concentration | 2 U/μl |
| Note |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA. This product is distributed for laboratory research only Caution: Not for diagnostic use. |
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