DNase I 脫氧核糖核酸酶I | Abmgood貨號G028
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特點
商品: DNase I | 貨號G028 2000 U (1.0 ml)
產品介紹: 脫氧核糖核酸酶I（DNase I; EC 3.1.21.1）是催化單鏈/雙鏈DNA，染色質和RNA-DNA雜交體中磷酸二酯鍵裂解的非特異性內切核酸酶。 DNA酶I切割DNA以釋放二 – 和/或寡核苷酸（5′-磷酸化和3′-羥基化）終產物。 一個單位定義；為在37℃下在10分鐘內催化1μgDNA降解為四核苷酸或更小片段的DNA酶I的量。產品應用: 從蛋白製劑和RNA樣品中去除DNA、介詞切口翻譯、生隨機片段用於雙脫氧測序、體外轉錄後降解模板DNA、介導DNA酶I印跡。
| Print Version | |
| Description | Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. |
| Application |
• Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
| Protocol |  |
| Concentration | 2 U/μl |
| Notes |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. This product is distributed for laboratory research onlyCaution: Not for diagnostic use. |