DNase I 脫氧核糖核酸酶I | Abmgood貨號G028
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商品: DNase I | 貨號G028 2000 U (1.0 ml)
產品介紹: 脫氧核糖核酸酶I（DNase I; EC 3.1.21.1）是催化單鏈/雙鏈DNA，染色質和RNA-DNA雜交體中磷酸二酯鍵裂解的非特異性內切核酸酶。 DNA酶I切割DNA以釋放二 – 和/或寡核苷酸（5′-磷酸化和3′-羥基化）終產物。 一個單位定義；為在37℃下在10分鐘內催化1μgDNA降解為四核苷酸或更小片段的DNA酶I的量。產品應用: 從蛋白製劑和RNA樣品中去除DNA、介詞切口翻譯、生隨機片段用於雙脫氧測序、體外轉錄後降解模板DNA、介導DNA酶I印跡。
| Description | Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. |
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| SKU | G028 |
| Unit quantity | 2000 U (1.0 ml) |
| Applications | • Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
| Concentration | 2 U/μl |
| Note |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA. This product is distributed for laboratory research onlyCaution: Not for diagnostic use. |