【生化檢測試劑盒 】Abbkine促銷優惠活動DM&產品資料
【細胞增殖&毒理&衰老】| DNA Damege損傷/細胞Stress | 細胞凋亡&細胞染色 | 發炎反應ELISA分析 | 膽固醇代謝/代謝試驗 | 生化檢測試劑盒 | 抗體蛋白純化&其它 | Tag 標籤單多株抗體
CheKine™ 組織和血液鹼性磷酸酶 (AKP/ALP) 活性比色測定試劑盒 | 貨號KTB1700
CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit
AKP/ALP是一種含鋅糖蛋白酶(zinc-containing glycoprotease)，可在鹼性環境(alkaline environment)中水解各種天然和合成的磷脂單酯化合物(phospholipid monoester compounds)。 AKP/ALP廣泛分佈於人體各個器官，主要是肝臟(liver)。在鹼性環境(alkaline environment)中，AKP/ALP催化鄰苯二甲酸二鈉(phthalate disodium)生成游離苯酚(free phenol)； 苯酚(phenol)與4-氨基安替比林(4-aminoantipyrine)和鐵氰化鉀反應(potassium ferricyanide)生成紅醌衍生物(red quinone derivative)，在510 nm處具有特徵光吸收； 吸光度增加率反映 AKP 活性。
| Product name | CheKine™ Tissue and Blood Alkaline Phosphatase (AKP/ALP) Activity Colorimetric Assay Kit |
| Applications notes | In an alkaline environment, AKP / ALP catalyzes phthalate disodium to generate free phenol; phenol reacts with 4-aminoantipyrine and potassium ferricyanide to produce a red quinone derivative, which has characteristic light absorption at 510 nm; the absorbance increase rate reflect AKP activity. |
| Kit components | • Extraction Buffer • Chromogen A • Chromogen B • Chromogen C • Standard |
| Features & Benefits | • Determination of alkaline phosphatase (AKP/ALP) activity in tissue lysates supernatants, plasma, serum, urine or purified enzyme samples. • Sample preparation, experimental steps and result calculation are more detailed and accurate. • Long shelf life. |
| Usage notes | • Assay kit is intended for research use only. Not for use in diagnostic procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. • Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted. • Avoid foaming or bubbles when mixing or reconstituting components. • Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. • Ensure all reagents and equipment are at the appropriate temperature before assay. • Chromogen A, Chromogen B and Chromogen C should be kept away from light. Chromogen C cannot be used after it turns blue-green. • After adding Chromogen C, it must be mixed immediately, otherwise the color development will not be complete. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
Tannase(TAN) 活性比色測定試劑盒 | CheKine™ Tannase(TAN) Activity Colorimetric Assay Kit貨號KTB1542
產品描述：
Tannase，全稱是Tannin Acyl Hydrolase（Tannin Acyl Hydrolase丹寧酶，EC 3.1.1.20）。單寧酶(Tannase)存在於富含單寧的植物(tannin-rich plants)中，也廣泛存在於微生物(microorganisms)中。鞣酸酶(Tannase hydrolyses)水解沒食子酸單寧(gallic acid tannins)中的酯鍵(ester bonds)和縮合鍵(depside bonds)，釋放沒食子酸(gallic acid)和葡萄糖(glucose)。該酶可以由黑曲霉(Aspergillus Niger)、米曲霉(Aspergillus oryzae)等黴菌產生。可用於處理啤酒中的單寧和蛋白質，使其清澈透明。還可用於去除柿子等產品的澀味。也可用於泡製速溶茶，防止發酵茶渾濁。
absorbance at 270 nm
0.3125-20 μmoL/mL
| Product name | CheKine™ Tannase(TAN) Activity Colorimetric Assay Kit |
| Kit components | •Extraction Buffer •Substrate •Standard |
| Features & Benefits | • Determination of plant tissue, fungi, bacteria and other samples. • Detailed sample preparation and result calculation methods are provided. • Provide standards and standard curves for reference. |
| Calibration range | 0.3125-20 μmoL/mL |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. |
| Storage instructions | Stored at 4℃ for 6 months, protected from light |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
CheKine™ 單寧比色測定試劑盒 | CheKine™ Tannin Colorimetric Assay Kit貨號KTB1541
產品描述：
單寧(Tannin)是一種廣泛存在於植物中的多酚類化合物(polyphenolic compounds)，又稱植物多酚(plant polyphenols)。一般有澀味(astringent taste)，能沉澱蛋白質(precipitate proteins)、生物鹼(alkaloids)、多醣(polysaccharides)，能與各種金屬離子(metal ions)發生絡合或靜電作用(electrostatically interact)。按化學結構(hemical structure)，單寧(Tannin)可分為水解單寧和縮合單寧。單寧(Tannin)與蛋白質結合的能力也稱為收斂性。它的澀味是各種生理活動的基礎，如止血、抑制微生物(microorganisms)、抗過敏(anti-allergy)、抗突變(anti-mutation)、抗腫瘤(anti-tumor)、抗衰老(anti-aging)等生理活動，也是影響味覺的因素之一。
absorbance at 760 nm
0.0156-1 mg/mL
| Product name | CheKine™ Tannin Colorimetric Assay Kit |
| Kit components | •Assay Buffer •Chromogen •Standard |
| Features & Benefits | • Determination of liquid samples such as plant tissue, juice and honey. • Detailed sample preparation and result calculation methods are provided. • Provide standards and standard curves for reference. |
| Calibration range | 0.0156-1 mg/mL |
| Storage instructions | Stored at 4℃ for 6 months, protected from light |
| Shipping | Gel pack with blue ice. |
CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒 | CheKine™ Aconitase (ACO) Activity Colorimetric Assay Kit貨號KTB1290
產品描述：
烏頭酸酶(Aconitase)是三羧酸循環(tricarboxylic acid cycle)中的一種酶，它催化檸檬酸(citric acid)轉化為異檸檬酸(isocitrate)。檸檬酸(Citric acid)本身不易被氧化。在烏頭酸酶(aconitase)的作用下，羥基(hydroxyl group)通過脫水加水反應從β碳原子(β carbon atom)轉移到α碳原子(α carbon atom)上，生成易脫氧的異檸檬酸(isocitric acid)，用於進一步的氧化脫羧反應(decarboxylation)製備。
absorption at 340 nm.
CheKine™ Aconitase (ACO) Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the Aconitase (ACO) Activity
| Product name | CheKine™ Aconitase (ACO) Activity Colorimetric Assay Kit |
| Applications notes | CheKine™ Aconitase (ACO) Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the Aconitase (ACO) Activity |
| Kit components | •Extraction Buffer •ReagentⅠ •ReagentⅡ •ReagentⅢ •ReagentⅣ •ReagentV •ReagenVI |
| Features & Benefits | • Determination of Aconitase (ACO) Activity in a variety of biological samples such as serum, plasma, tissues, cells and plants. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
產品描述：
醇醯基轉移酶（Alcohol Acyltransferase ,AAT）是一個多功能蛋白大家族，主要負責催化體內的各種醯化(acylation)和脫醯化反應(deacylation reactions)，在基因表達(gene expression)、代謝(metabolism)和信號轉導(signal transduction)中發揮重要作用。CheKine™ 醇醯基轉移酶（AAT）活性比色檢測試劑盒為靈敏檢測醇醯基轉移酶（Alcohol Acyltransferase , AAT）活性提供了方便的工具。
| Product name | CheKine™ Alcohol Acyltransferase（AAT）Activity Colorimetric Assay Kit |
| Kit components | •Extraction Buffer •Receptor •Substrate • Chromogen |
| Features & Benefits | • Determination of Alcohol Acyltransferase（AAT）Activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. |
| Usage notes | • 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. |
| Storage instructions | Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
CheKine™黃嘌呤氧化酶檢測 | CheKine™ Xanthine Oxidase Assay Kit貨號KTB1070
黃嘌呤氧化酶（Xanthine oxidase , EC 1.17.3.2）或黃嘌呤氧化還原酶(xanthine oxidoreductase) 被認為是嘌呤(purine)在人體內分解代謝的末端酶，可催化次黃嘌呤(hypoxanthine)氧化為黃嘌呤(xanthine)然後變為尿酸(uric acid)。 當充當為NADH氧化酶 (NADH oxidase) 時，XO是超氧化物（superoxide , O2-）的生成器(generator)，超氧化物（superoxide , O2-）是一種強大的活性氧（reactive oxygen species , ROS）。 還已經發現XO在缺血再灌注損傷(ischemia-reperfusion injury)期間會產生過氧化氫（hydrogen peroxide , H2O2）和超氧化物（superoxide , O2-）。 由於它們的高反應性，這些ROS影響細胞的各種分子成分，過量會導致細胞變性(cell degeneration)和死亡(death)。 XO（Xanthine oxidase , EC 1.17.3.2）在健康個體的肝臟(liver)和空腸(jejunum)中存在大量。 但是，在各種肝臟疾病(liver disorders)中，已發現循環XO的水平急劇增加。 因此，血清XO(serum XO)水平的測定可作為急性肝損傷(acute liver damage), 如黃疸( jaundice）的敏感指標。
產品描述： CheKine™黃嘌呤氧化酶檢測試劑盒(CheKine™ Xanthine Oxidase Assay Kit, KTB1070) 提供了一種簡便的比色測定法，用於定量測定各種樣品中存在的黃嘌呤氧化酶 （Xanthine oxidase, XO）。 在測定中，黃嘌呤氧化酶 （Xanthine oxidase, XO）將黃嘌呤氧化成超氧化物（superoxide , O2-）。 超氧化物（superoxide , O2-）與四唑鹽WST-8 (tetrazolium salt WST-8) 染料反應形成水溶性的formazan product產物，可以很容易地在OD 450 nm處進行比色定量。 因此，樣品中存在的黃嘌呤氧化酶 （Xanthine oxidase, XO）與所獲得的訊號成比例。線性範圍15.6 -500 mU/mL.
Kit components
- Assay Buffer
• Sample Diluent
• WST-8
• Enhancer
• Xanthine Oxidase Standard(10 U/mL)
• Xanthine
β-澱粉酶活性比色測定試劑盒 |β- Amylase Activity Colorimetric Assay Kit貨號KTB1380
產品描述：
澱粉水解酶(Starch hydrolases)，包括α-澱粉酶（α -amylase, α-Al，EC 3.2.1.1）和β-澱粉酶（β -amylase, β-Al）。 α-澱粉酶(α -amylase)隨機催化澱粉中的α-1,4-糖苷鍵(α-1, 4-glycosidic bond)水解(hydrolysis)生成葡萄糖(glucose)、麥芽糖(maltose)、糊精(dextrin)等還原糖(reducing sugars)，同時降低澱粉(starch)的粘度(viscosity)，故又稱液化酶(liquefaction enzyme)。
CheKine™ β-澱粉酶活性比色測定試劑盒(CheKine™ β-Amylase Activity Colorimetric Assay Kit, KTB1380) 為檢測 β-澱粉酶活性(β-Amylase Activity)提供了方便的工具。其原理是澱粉酶(amylase)催化澱粉(starch)水解生成還原糖(reducing sugar)，還原3, 5-二硝基水楊酸(3, 5-dinitrosalicylic acid)生成棕紅色物質，在540 nm處有吸收峰。通過測量 540 nm 處的吸光度增加率來計算澱粉酶活性(Amylase activity)。
| Product name | CheKine™ β-Amylase Activity Colorimetric Assay Kit |
| Applications notes | CheKine™ β-Amylase Activity Colorimetric Assay Kit provides a convenient tool for detection of β-Amylase Activity. The principle is that amylase catalyzes the hydrolysis of starch to produce reducing sugar, which reduces 3, 5-dinitrosalicylic acid to produce brown-red material, with an absorption peak at 540 nm. Amylase activity was calculated by measuring the absorbance increase rate at 540 nm. |
| Kit components | •DNS Reagent •Substrate •Standard |
| Features & Benefits | • Determination of β-Amylase Activity in a variety of biological samples such as serum, plasma, animal and plant tissues. • Detailed sample preparation and result calculation methods are provided. • Standard and standard curve line are provided. |
| Calibration range | 0.0156-1 mg/mL |
| Limit of detection | 0.0078 mg/mL |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Blue ice |
| Background | Amylases are responsible for the hydrolysis of starch, mainly consisting of α -amylase (α -Al) and β -amylase (β -Al, EC 3.2.1.2). β -amylase cleaved the α-1, 4-glycosidic bond from the non-reducing end of starch to produce reducing sugars such as glucose, maltose, maltose trisose and dextrin. |
葡萄糖氧化酶活性測定 | Glucose Oxidase Activity (GOD) Assay Kit貨號KTB1310
CheKine™ Glucose Oxidase Activity (GOD) Assay Kit 偵測範圍: 0.05 – 2 U/L, 最低靈敏度0.05 U/L
葡萄糖氧化酶 (glucose oxidase enzyme , GOx) (EC 1.1.3.4) 是一種二聚酶(dimeric enzyme)，可催化 β-D-葡萄糖(beta-D-glucose)氧化成過氧化氫(hydrogen peroxide)和 D-葡萄糖酸-1,5-內酯(D-glucono-1,5-lactone)，後者被水解成葡萄糖酸(gluconic acid)。 這種酶是由某些種類的昆蟲(insects)、真菌(fungi)和細菌(bacteria)產生的。 葡萄糖氧化酶(Glucose oxidase)廣泛用於測定體液中的葡萄糖以及去除飲料、食品和其他農產品中殘留的葡萄糖和氧氣(oxygen)。 此外，葡萄糖氧化酶(Glucose oxidase)通常用於生物傳感器(biosensors)以檢測葡萄糖。
CheKine™ 葡萄糖氧化酶活性測定試劑盒(CheKine™ Glucose Oxidase Activity Assay Kit, KT1310)提供了一種簡單易行的比色測定法，用於檢測生物樣品中的葡萄糖氧化酶活性(Glucose Oxidase Activity)，例如血清或血漿、細胞、尿液、其他體液、食物、生長培養基中的葡萄糖氧化酶活性。 在該測定中，GOD 活性通過偶聯酶測定確定，其中 GOD 氧化 D-葡萄糖(D-glucose)，導致產生過氧化氫 (hydrogen peroxide , H2O2)，該過氧化氫 (hydrogen peroxide , H2O2) 在酸性條件下與色原反應，產生可在 OD 580 下測量的產物。 因此，樣品中存在的葡萄糖氧化酶活性與獲得的信號成正比。偵測範圍: 0.05 – 2 U/L, 最低靈敏度0.05 U/L。可應用於以下樣品來源: serum or plasma, cells, urine, other body fluid, food, growth medium。
| 產品貨號 | 產品品項 | 反應 | 測量儀器 |
| 貨號KTB1700 | 組織和血液鹼性磷酸酶（ Alkaline Phosphatase, AKP / ALP）比色測定 | 96個反應/ | 吸收光510 nm |
| 貨號KTB1542 | Tannase(TAN) 活性比色測定試劑盒 | 48個反應/ |
absorbance at 270 nm 0.3125-20 μmoL/mL |
| 貨號KTB1541 | CheKine™ 單寧比色測定試劑盒 | CheKine™ Tannin Colorimetric Assay Kit | 48個反應/ |
absorbance at 760 nm 0.0156-1 mg/mL |
| 貨號KTB1290 | CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒 | 48個反應/ | absorption at 340 nm. |
| 貨號KTB1560 | 醇醯基轉移酶（AAT）活性比色檢測試劑盒 |
48個反應/ 96個反應 |
absorption at 412 nm. |
| 貨號KTB1070 | CheKine™黃嘌呤氧化酶檢測 | 96個反應 |
OD 450 nm 線性範圍15.6 -500 mU/ |
| 貨號KTB1380 | β-澱粉酶活性比色測定 | 48個反應 |
absorption peak at 540 nm 0.0156-1 mg/mL |
| 貨號KTB1310 | 葡萄糖氧化酶活性測定 | 48個反應 |
OD 580 下測量 偵測範圍: 0.05 – 2 U/L |