Chicken Anti-Rat IgG (H+L)  |貨號ARatIG (未標誌)

雞非哺乳類動物，和哺乳類抗原有高親和性，且避免和哺乳類抗原產生cross-reaction。為最人道的多株抗體產製方法。只需從雞蛋萃取蛋白，取樣方便，不傷害動物。每隻雞可以產生的抗體量約3 g IgY／月，是兔子的10-20倍多。且產製速度比起兔子快上許多，最快25天即可取得高力價（titer）抗體。雞IgY的Fc region和哺乳類IgG的不同：可降低對哺乳動物的背景值。不會活化哺乳類補補體系統。不會與哺乳類Fc receptors結合。無法和常規的protein A（Staphylococcus aureus）、protein G（Streptococcus sp.）、或protein L（Peptostreptococcus magnus）等免疫球蛋白－蛋白結合反應。由於IgY取得方便，雞蛋可以儲存較長的時間，並可純化取得較佳的titer。相對於兔子，雞的開銷較便宜。IgY有以下數種和哺乳類IgG類似的特徵：為二價體。可由papain分解成divalent Fab fragment。適用酵素、生物素（biotin）、金粒子等標定物。

Full Name	Affinity-purified Chicken-anti-Rat IgG (H+L)
Host	Chicken
Quantity	0.5 mg
Volume	0.5 mL
Type	Affinity purified
Physical State	Liquid
Concentration	1.0 mg/mL
Diluent	Phosphate buffered saline‚ pH 7.2
Preservative	0.1% Sodium Azide
Procedure	Chickens were immunized with highly purified rat IgG isolated from normal pooled rat serum. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent.
Specifications	The affinity purified anti rat IgG reacts with immunoglobulins and the papain derived Fc and Fab fragments by IEP and gel diffusion techniques.
Storage	4°C
Note	For in vitro diagnostic‚ research or proteomics applications only.

貨號	IgY抗體商品	(Affinity purified)
DAIgYFab	Donkey Anti-Chicken IgY Fab	0.5 mg
DAIgYFab-B	Donkey  Biotinylated Anti-Chicken IgY Fab	0.5 mg (BIOTIN)
DAIgYFab-F	Donkey  FITC-labeled Anti-Chicken IgY Fab	0.5 mg (FITC)
DAIgYFab-HRP	Donkey  HRP-labeled Anti-Chicken IgY Fab	0.5 mg (HRP)
AIgYFab	Goat Anti-Chicken IgY Fab	0.5 mg