血小板衍生生長因子亞單位 A (PDGFA) ELISA 試劑盒 | USCN貨號USEA528Hu
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血小板衍生生長因子亞單位 A (PDGFA) ELISA 試劑盒 | USCN貨號USEA528Hu
ELISA Kit for Platelet Derived Growth Factor Subunit A (PDGFA)
商品: 血小板衍生生長因子亞單位 A (PDGFA) ELISA 試劑盒 | Double-antibody Sandwich ELISA貨號USEA528Hu，應用物種為Bos taurus; Bovine (Cattle) Same name, Different species.；偵測的靈敏度15.6-1,000pg/mL、最低靈敏度6.1pg/mL。可應用於Serum、plasma、tissue homogenates、cell lysates、milk、cell culture supernates and other biological fluids之樣品來源。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。USCN提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。此產品需由儀器進行定量 ELISA儀器設備請按此
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器
血小板衍生生長因子亞單位 A (PDGFA) ELISA 試劑盒的穩定性
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
血小板衍生生長因子次單位 A (PDGFA) ELISA 試劑盒檢測流程摘要
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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