PDE5A ELISA 試劑盒 (酵素免疫吸附試驗法) | USCN貨號USEF645Hu
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磷酸二酯酶 5A cGMP 特異性 ELISA 試劑盒 (PDE5A) (酵素免疫吸附試驗法) | USCN貨號USEF645Hu
ELISA Kit for Phosphodiesterase 5A, cGMP Specific (PDE5A)
商品: PDE5A ELISA檢測試劑盒 (酶聯免疫吸附試驗法) | Double-antibody Sandwich貨號USEF645Hu，應用物種Homo sapiens (Human) ；偵測範圍1.56-100ng/mL, 最低靈敏度 0.55ng/mL. 可應用於Homo sapiens (Human) 生物樣品之檢測。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。USCN提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。
磷酸二酯酶 5A cGMP 檢測試劑盒套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary of the ELISA Kit for PDE5A
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
PDE5A相關之抗體 | 蛋白質 | ELISA | CLIA產品
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| URPF645Hu01 | Recombinant Phosphodiesterase 5A, cGMP Specific (PDE5A) | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAF645Hu01 | Polyclonal Antibody to Phosphodiesterase 5A, cGMP Specific (PDE5A) | WB |
| UMAF645Hu21 | Monoclonal Antibody to Phosphodiesterase 5A, cGMP Specific (PDE5A) | WB; IHC; ICC; IP. |
| USEF645Hu | ELISA Kit for Phosphodiesterase 5A, cGMP Specific (PDE5A) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMF645Hu | Multiplex Assay Kit for Phosphodiesterase 5A, cGMP Specific (PDE5A) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |