Actinomycin D | APExBIO貨號 A4448
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Actinomycin D | APExBIO貨號 A4448
RNA polymerase inhibitor
IC50：放線菌素 D (Actinomycin D) 表現出濃度依賴性 DNA 修復活性降低，IC50 為 0.42 μM [1]。放線菌素 D (dactinomycin) 是放線菌素的一種，是從鏈黴菌屬土壤細菌分離出來的一類多肽抗生素。放線菌素 D (Actinomycin D) 作為一種較老的化療藥物(older chemotherapy)已經使用多年，它與雙鍊和單鏈 DNA 結合，透過在轉錄起始複合物處結合 DNA 並阻止 RNA 聚合酶延長 RNA 鏈來抑制 DNA 和 RNA 的合成。體外：先前的研究旨在確定放線菌素 D (Actinomycin D) 對原代培養 24 小時期間分離的大鼠脂肪細胞釋放瘦素(leptin)的影響。結果表明，放線菌素 D (Actinomycin D) 和地塞米松均可減少 24 小時孵育期間瘦素 mRNA 的損失。僅需 0.1 μM 的放線菌素 D (Actinomycin D) 即可對瘦素釋放和瘦素 mRNA 累積產生最大影響，該濃度對 24 小時孵育結束時脂肪細胞的 18S RNA 含量沒有顯著影響。與 24 小時時觀察到的瘦素 mRNA 損失減少相反，在 0.1 μM 放線菌素 D (Actinomycin D) 存在的情況下，3-磷酸甘油醛脫氫酶信使核糖核酸 (GAPDH mRNA) 的損失增加。這些結果證明了放線菌素 D (Actinomycin D) 對瘦素釋放和瘦素 mRNA 水平的獨特調節 [2]。
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APExBIO提供優質的胜肽和生物分析試劑，廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域， 還提供定制的服務，包括多肽合成，抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析，高效液相色譜，質譜，和HMNR數據。>>更多APExBio商品
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