雙螢光素酶報告基因檢測試劑盒 |Dual Luciferase Reporter Gene Assay Kit貨號KTA8010

Dual Luciferase Reporter Gene Assay Kit

產品描述：雙螢光素酶報告基因檢測試劑盒( Dual Luciferase Reporter Gene Assay Kit, KTA8010) 具有靈敏、快速、方便，可檢測雙報告基因( Reporter gene )。 雙螢光素酶報告基因檢測系統提供了執行兩種報告基因檢測的有效方法。從單一樣本依序測量螢火蟲 ( Firefly Luciferase ) 和海腎 螢光素酶( Renilla Luciferase)的活性。透過檢測螢光素酶(Luciferase)的活性來判斷藥物治療對目標基因的轉錄調控作用。海腎螢光素酶(Renilla luciferase)檢測轉染效率的內參( internal reference )，以消除細胞數量與轉染效率之間的差異。

螢火蟲螢光素酶( Firefly luciferase)可以催化螢火蟲螢光素(firefly luciferin)生成氧化螢光素(Oxyluciferin) (在ATP、鎂離子和氧氣存在的情況)，氧化螢光素( oxidation of luciferin)過程產生光訊號。在有氧(oxygen)存在的情況下，海腎螢光素酶(Renilla luciferase)可以催化coelenterazine氧化為coelenteramide， coelenterine氧化過程中也會產生光訊號。透過螢光素酶及其底物的生物發光系統，可以非常靈敏且有效率。通常，將目的基因的轉錄調控元件( Transcriptional regulation element)或5′-啟動子區(5′-promoter region)克隆到螢光素酶(Luciferase )上游，或將3′-UTR區克隆到螢光素酶(Luciferase )下游，建構報告基因質體 ( Reporter gene plasmid)，然後進行轉染(transfection)細胞，處理用適當的藥物細胞，然後裂解細胞。透過檢測螢光素酶(Luciferase)的活性來判斷藥物治療對目標基因的轉錄調控作用。海腎螢光素酶(Renilla luciferase)用作檢測轉染效率的內參( internal reference )，以消除細胞數量與轉染效率之間的差異。雙螢光素酶報告基因檢測試劑盒首先以螢光素(Luciferin)為底物檢測螢火蟲螢光素酶(Firefly luciferase)的活性，然後添加抑制螢火蟲螢光素酶(Fireflyluciferase)催化的物質，同時添加 Coelenterazine 檢測海腎螢光素酶( Renilla luciferase)的活性，從而實現雙螢光素酶的檢測螢光素酶報告基因。該試劑盒具有檢測快速、靈敏度高、檢測範圍廣、不受細胞內活性干擾的特性。

Product name	Dual Luciferase Reporter Gene Assay Kit
Applications notes	Usually, the Transcriptional regulation element or 5′-promoter region of the gene of interest is cloned upstream of Luciferase, or the 3′- UTR region is cloned downstream of Luciferase to construct a Reporter gene plasmid, and then transfect the cell, treat the cell with appropriate drugs, and then Lytic cells. The Transcriptional regulation effect of drug treatment on the target gene is judged by detecting the activity of Luciferase.
Kit components	•Lysis Buffer (5×)•Firefly Luciferase Assay Buffer•Firefly Luciferase Substrate•Renilla Luciferase Assay Buffer•Renilla Luciferase Substrate (50×)
Features & Benefits	• High luminous intensity. • High detection sensitivity, wide linear range of the measured sample. • Easy operation, stable reading, fast speed. • Strong compatibility with various media.
Storage instructions	Stored at -20℃ for 12 months, protected from light
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	In the presence of ATP, magnesium ions and oxygen, Firefly luciferase can catalyze firefly luciferin to Oxyluciferin, which generate light signals during the oxidation of luciferin. In the presence of oxygen, Renilla luciferase can catalyze the oxidation of coelenterazine to coelenteramide, and light signals are also generated during the oxidation of coelenterine. Through the Bioluminescence system of Luciferase and its substrate, gene expression can be detected very sensitively and efficiently.