鞘磷脂(SPH) ELISA 試劑盒 (通用型) | 貨號CK-bio-25442
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鞘磷脂(Sphingomyelin, SPH) ELISA 試劑盒 (通用型) | 貨號CK-bio-25442
General Sphingomyelin(Sphingomyelin, SPH) ELISA Kit
偵測範圍: 5ng/mL -100ng/mL , 最低靈敏度1.0 ng/mL
鞘磷脂(Sphingomyelin, SPH)檢測酶聯免疫吸附測定（ELISA）試劑盒是一種體外測定鞘磷脂(Sphingomyelin, SPH)的定量測定。是一種競爭性酶免疫測定試劑盒(competitive method)。將 Standard 和 Sample 添加到預先包被目的抗體的孔中，然後加入鏈黴親和素 HRP 形成免疫複合物，孵育，通過孵育和洗滌，去除未結合的酶，然後加入底物A和B，則溶液在酸的作用下會變成藍色，最後變成黃色。顏色深淺與檢測鞘磷脂(Sphingomyelin, SPH)的濃度呈負相關。 5ng/mL -100ng/mL , 最低靈敏度1.0 ng/mL。注意事項： 5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免失去生物活性，避免污染及反覆冷凍。
Double speed Elisa 試劑盒在 90 -120分鐘內即可快速獲得實驗結果；競爭法(competitive method)2-3小時。 保持著卓越的偵測專一性與靈敏度。即用型標準品含有6瓶濃度，不需自行配製。每瓶 0.5ml (濃度依不同產品有所不同) 。
鞘磷脂(Sphingomyelin, SPH)試劑盒
| Name | General Sphingomyelin(SPH) ELISA Kit |
| Cat No. | CK-bio-25442 |
| Specification | 48T/96T/5*96T/10*96T |
| Detection range | 5ng/mL -100ng/mL |
| Detection limit | 1.0 ng/mL |
| Test principle | Quantitative |
| Test method | competitive method |
| Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Sample type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
For samples:Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
For the quantitative determination of Aflatoxin B1(Aflatoxin B1) concentrations in serum, plasma, saliva, urine, tissue homogenate, cell culture supernates,body fluid and other biological fluids
Note: 提供己配製好的標準品6瓶 Standard (A → F) concentration was followed by: 0ng/mL,6.25ng/mL ,12.5ng/mL ,25ng/mL ,50ng/mL ,100ng/mL
樣品準備要求 (Specimen Requirements)-血清/血漿/細胞/尿液 (實際操作以原廠操作手冊為主)
5天內使用的樣品可在2-8℃保存，否則樣品必須在-20℃（≤1個月）或-80℃（≤2個月）保存，以免生物活性喪失，避免污染。
1.血清 (Serum)：
讓(Serum)在室溫下凝結10-20分鐘。離心（2000-3000 RPM）20 分鐘。仔細收集上清液(supernatants)。儲存過程中出現沉澱時，應重新離心。
2.血漿(Blood plasma)：
根據樣本採集要求，使用EDTA或檸檬酸鈉(sodium citrate)抗凝(anti coagulation)。添加 EDTA 或檸檬酸鈉並混合 10-20 分鐘。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
3.尿液(Urine)：
用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。採集胸腹水、腦脊液時，請按上述程序進行。
4.細胞培養上清液(Cell culture supernatant)：
檢查分泌成分(secrete components)時用無菌管收集。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。檢查細胞內成分時，用PBS（PH 7.2-7.4）將細胞懸液稀釋至細胞濃度約100萬/ml。通過反覆凍融循環損壞細胞，釋放內部成分。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。儲存過程中出現沉澱時，應重新離心。
5.組織樣品(Tissue sample)：
切取樣品並稱重。添加一定量的PBS（PH 7.4）。立即用液氮冷凍備用。解凍樣品並保存在2-8℃。加入一定量的PBS（PH 7.4），然後用手或均質器將樣品徹底均質。離心（2000-3000 RPM）約 20 分鐘。仔細收集上清液。等分並保留一份用於檢查，其餘的冷凍以備後用。
Coon-koon台灣代理商-BRAND/AGENCY/代理品牌-Coon-koon
http://www.coonkoonbio.com/
Shanghai Coon Koon Biotech Co.,Ltd是一家專業從事生物製品研發、生產和經營的綜合性生物公司！ 產品遠銷多個國家Coon Koon Bio主要做ELISA試劑盒、生化試劑、抗體等上萬種科研產品！涉及分子生物學、細胞生物學、免疫學等各個領域！堅持“以質量為前提，以客戶滿意為宗旨”；確立“以客戶需求為導向，以市場需求為導向，不斷開發優質新產品，提供客戶滿意。
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