長鏈碳花青染料DiO (DiOC18(3)) | CAS#34215-57-1 貨號BMD0072

Recommendedworkingconcentrations: 5-10μM

DiO 是一種長鏈碳花青染料。碳花青染料廣泛用作 Di 來標記細胞、細胞器、脂質體、病毒和脂蛋白。綠色螢光親脂性碳花青 DiOC18(3) 被廣泛用作親脂性示踪劑(lipophilic carbocyanine)。它在水中的螢光很弱，但當摻入膜中時螢光很高，並且具有相當的光穩定性。它在脂質環境中具有極高的消光係數和短的激發態壽命（~1 納秒）。一旦應用於細胞，染料就會在質膜內橫向擴散。
碳花青染料廣泛用作 Di 來標記細胞、細胞器、脂質體、病毒和脂蛋白。長鏈碳花青(Carbocyanine dyes )包括DiO (DiOC18(3))、DiI (DiIC18(3))、DiD (DiIC18(5)) 和DiR，以及二烷基氨基苯乙烯基染料DiA (4-Di-16-ASP ) 用於標記膜和其他疏水結構。DiO、DiI、DiD 和 DiR 分別表現出明顯的綠色、橙色、紅色和紅外螢光; 從而促進活細胞的多色成像和流式細胞術分析。 DiO 和 DiI 可分別與標準 FITC 和 TRITC 濾光片一起使用。其中 DiI 及其類似物最常用，因為它們通常表現出非常低的細胞毒性。此外，DiI 還廣泛用於測定脂蛋白，例如 LDL 和 HDL。親脂性氨基苯乙烯基染料 DiA 也常用於神經元示踪[2]。The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Specification

Product name	DiO (DiOC18(3))
CAS Number	34215-57-1
Molecular Formula	C53H85ClN2O6
Alternative	DiO (DiOC18(3)),BMD00072

Product Properties

Formulation	Yellow solid
Features & Benefits	• λEX/λEm: 484/501 nm(MeOH)
Molecular weight	882
Usage notes	• Soluble in ethanol, anhydrous DMSO, and anhydrous DMF, with a solubility of approximately 10 mg/mL in anhydrous DMSO. When it is difficult to dissolve, it can be properly heated or ultrasonically treated.
Storage instructions	Store at 4°C. The product can be stored for up to 12 months. Protect from light.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background

The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.

Alternative

DiO (DiOC18(3)),BMD00072

Image & description