Minute™ 核膜蛋白提取試劑盒 |Nuclear Envelope Protein Extraction Kit Invent貨號NE-013
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快速蛋白質提取試劑盒系列-Rapid Protein Extraction Kits
Minute™蛋白萃取套組，使用創新『Column Filter』技術，效果更佳，完成只需 一 分鐘蛋白萃取只需要vortex、transfer、spin 。避免 RIPA 不溶性蛋白質的問題基於旋轉柱的方法獲得更完整的蛋白質譜。Minute ™一系列蛋白質提取試劑盒；適用於多種物種（包括哺乳動物、海洋動物、昆蟲、植物和細菌）快速和容易地提取/分離蛋白質的優異工具 。與市場上的任何其他蛋白質提取試劑盒不同，優化的緩衝液系統，結合最新的專有濾筒技術（spin column）；快速提高蛋白萃取的效能及方便性；用於總蛋白提取，細胞分離和膜蛋白分離，只需簡單四步驟即可完成蛋白質萃取。無須長時間等待、免除重複性的wash步驟。
Minute™ 核膜蛋白提取試劑盒 | Invent貨號NE-013
Minute™ Nuclear Envelope Protein Extraction Kit (50 Preps)
手冊和協議 | 材料安全數據表 (MSDS) |參考文獻 (4)
核膜是一個非常複雜的膜蛋白系統，由於其與核和細胞質成分的連接而很難分離和純化。傳統的核膜分離和純化方法需要大量的起始材料和漫長而繁瑣的過程。Minute™ 核膜蛋白提取試劑盒是第一個商業試劑盒，旨在快速分離天然形式的核膜及其相關蛋白，無需使用密度梯度和超速離心。由於使用蛋白質提取濾芯，核膜蛋白分離簡單且用戶友好。核膜蛋白在最終製備中顯著富集。與需要大量起始細胞/組織的傳統方法不同，該試劑盒起始僅包含 10-2000 萬個細胞，並且緩衝液不含去污劑和 EDTA。該過程可在 45 分鐘內完成，最終產量為 10-50 µg 蛋白質/樣品。
The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure. Minute™ Nuclear Envelope Protein Extraction Kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultracentrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods that require large amounts of starting cells/tissues, this kit starts with only 10-20 million cells, and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 minutes with a final yield of 10-50 µg protein/sample.
A. 分離的小鼠脾細胞的 SDS-PAGE 譜和蛋白質印跡。泳道 1 和 2，總細胞裂解液；泳道3，核膜。
B.Rate (REL-6TN) 和人 (MCF-7) 細胞系的 SDS-PAGE 譜和蛋白質印跡。泳道1，REL-6TN總細胞裂解液；泳道 2，REL-6TN 核膜。泳道3，MCF-7總細胞裂解液；泳道 4，MCF-7 核膜。核膜標記物 Lamin B1（ab 16048，ABcam，Cambridge MA）和 GAPDH（G9545，Sigma）通過比色底物（Bio-Rad）可視化。
A. SDS-PAGE profiles and Western blottings of isolated mouse spleen cells. Lanes 1 and 2, total cell lysate; Lane 3, nuclear envelope.
B. SDS-PAGE profiles and Western blottings of Rate (REL-6TN) and human (MCF-7) cell lines. Lane 1, REL-6TN total cell lysate; Lane 2, REL-6TN nuclear envelope. Lane 3, MCF-7 total cell lysate; Lane 4, MCF-7 nuclear envelope. Nuclear envelope marker Lamin B1 (ab 16048, ABcam, Cambridge MA) and GAPDH (G9545, Sigma) were visualized by a color metric substrate (Bio-Rad).
Minute™ 核膜蛋白提取試劑 Kit includes:
| Items | Quantity |
| Buffer A | 25 ml |
| Buffer B | 15 ml |
| Filter Cartridges | 50 units |
| Collection Tubes with Caps | 50 units |
基於旋轉柱(SPIN-COLUMN-BASED )的蛋白質提取/細胞分級(CELL FRACTIONATION)
Invent Biotechnologies 成立於 2010 年，因革新蛋白質提取(protein extraction)和細胞分離(cell fractionation)而享譽全球。我們開發了基於旋轉柱(spin column-based)的創新技術，用於蛋白質研究( protein research)，可簡化和增強樣品製備。這會帶來更順暢的工作流程和始終可靠的結果，從而帶來卓越的數據質量。選擇 Minute™ 試劑盒進行蛋白質提取(protein extraction)和細胞分級分離(cell fractionation)的研究人員可以相信，他們的樣品準確地代表了所研究的細胞或組織的自然特徵。這些套件只需要一台台式離心機，最大限度地減少實驗變量並確保高質量的材料以獲得更可靠的數據。Minute™ 方法的有效性得到了越來越多 journals的支持 (Nature, Science, and Cell.)。
The Minute™ 的優點：
• 超快（快至 1 分鐘）
• 樣本量小
• 最小化交叉污染
• 內源性基線不變
• 所有活體和組織類型
The Minute™ 應用：
• 蛋白質運輸/易位
• 蛋白質-蛋白質相互作用
• 蛋白質修飾
• 信號轉導
• sc/snRNA-seq
Invent Minute™ 徹底改變蛋白質樣品製備和細胞分級分離
自 2010 年成立以來，我們的首要目標是利用我們基於旋轉柱的創新技術徹底改變全球蛋白質提取(protein extraction)和細胞分離(cell fractionation)市場。我們的目標是讓 Minute™ 試劑盒成為標準實驗室程序，取代所有基於溶液的樣品製備方法。通過利用這些套件，研究人員只需一台台式離心機即可獲得一致的結果並獲得卓越的數據質量。
改變蛋白質提取(Transforming protein extraction)
Minute™ 總蛋白提取試劑盒採用簡單的旋轉柱( simple spin column )，可有效消除細胞或組織裂解過程(lysis)中的非系統性蛋白質損失(non-systematic protein loss)。這代表著相對於傳統的基於解決方案的方法，例如 RIPA 緩衝液(RIPA buffer)和超聲處理(sonication)的顯著進步，這些方法以其可變性和無法提供全面的蛋白質譜而聞名。用於總蛋白提取的 Minute™ 試劑盒與來自各種生物體和組織類型的小樣本量兼容，為研究人員提供了未改變的內源蛋白基線( endogenous proteins)，從而實現更精確的定量和分析。
Invent Biotech’s Minute Kit shows a more complete cell lysis compared to RIPA buffer, in which a pellet remains, leading to an alteration of the protein profile.
改進細胞分級分離(Revamping cell fractionation)
由於缺乏簡單的實驗方案，傳統的細胞分級分離方法長期以來一直受到用戶偏差和結果不一致的影響。 Minute™ 細胞分離試劑盒徹底改變了這一過程，使研究人員能夠在 90 分鐘內獲得高純度的細胞器群，並且使用最少的洗滌劑。通過採用簡單的旋轉柱，這些試劑盒無需均質化(homogenization)、密度梯度分離(density gradient separation)和超速離心等傳統技術( ultra-centrifugation)，從而減少了實驗變量的數量。這可以防止細胞器損傷( organelle damage)並產生更一致和生理相關的結果。憑藉多種 Minute™ 試劑盒可滿足不同的細胞分級(cell fractionation)分離要求，我們正在推動蛋白質組研究的轉型。
支持所有研究學科(Supporting all research disciplines)
Minute™ 試劑盒旨在無縫集成到現有的蛋白質提取和細胞分級分離工作流程中，提供快速且一致的性能，確保可靠的結果。然而，不要只相信我們的話——我們的產品已在科學文獻中被廣泛引用，這表明研究界對簡化且可靠的樣品製備方法的需求不斷增長。立即切換到 Minute™，體驗我們技術的優勢，無論您的研究領域如何。
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