EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) APExBIO貨號R1010
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特點
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) APExBIO貨號R1010
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)
產品介紹
EZ Cap™ Cy5 螢火蟲螢光素酶 mRNA (5-moUTP) 一旦進入最初從螢火蟲 Photinus pyralis 中提取的細胞就會表達螢光素酶蛋白。該酶催化 ATP 依賴性 D-螢光素氧化並導致在約 560 nm 波長處產生化學發光。螢火蟲螢光素酶是一種常用的生物發光報告基因，用於基因調控和功能研究。適用於 mRNA 傳遞( mRNA delivery)、翻譯效率(cell viability)、細胞活力(cell viability)和體內成像等檢測。EZ Cap™ Cy5 螢火蟲螢光素酶 mRNA (5-moUTP) 的濃度為 1 毫克/毫升。它由 EZ Cap™ Reagent AG (3′ OMe)（貨號 B8178）共轉錄封端，可高效生成帽 1 結構。 Cap 1 結構更適合哺乳動物系統，轉錄效率高於 Cap 0 結構（ARCA 和 mCap）。 EZ Cap™ Reagent AG (3′ OMe) 獲得了僅以正確方向插入的能力，導致形成的 mRNA 的翻譯效率是使用 EZ Cap™ Reagent AG 啟動的 mRNA 的兩倍。添加 5-moUTP (5-Methoxy-UTP) 和 poly(A) 尾巴可抑制 RNA 介導的先天免疫激活並增加 mRNA 在體外和體內的穩定性和壽命。 Poly(A) 尾在提高翻譯起始效率方面也起著重要作用。 Cy5 是一種合成的紅色螢光染料，最大激發和發射波長分別為 650 nm 和 670 nm。 Cy5-UTP 和 5-moUTP 在轉錄時以 1:3 的比例使用。按此比例進行替換會產生易於可視化且仍可在細胞培養物中翻譯的 mRNA。粗略地說，翻譯效率和 Cy5-UTP 替換之間存在反相關。所有的修改都是為了模仿一個完全加工的成熟 mRNA。 EZ Cap™ Cy5 螢火蟲螢光素酶 mRNA (5-moUTP) 是觀察 mRNA 傳遞(mRNA delivery)、定位(localization)、翻譯( translation a)和其他行為的理想產品。
| mRNA Length | 1921 nucleotides | ||
| Concentration | 1 mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | Dissolve it on ice and take care to prevent RNase contamination degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For the first time, it is gently centrifuged and divided into several parts for stand-alone use. Use RNase-free reagents and consumables, using appropriate RNase-free technology. It can not be added to the serum-containing medium until it is mixed with the transfection reagent. | ||
| Shipping Condition | Evaluation sample solution : ship with dry ice. | ||
APExBIO提供優質的胜肽和生物分析試劑，廣泛產品線如Protease、Chromatin/Epigenetics表觀遺傳學、MAPK Signal、DNA Damege/DNA repair、細胞凋亡、腫瘤生物學、Stem cell等研究領域， 還提供定制的服務，包括多肽合成，抗體的生產和檢測的發展。產品線DNA/RNA prep kit、Drug Screening panel、Bioactive peptides、Growth factors、Biotinylation Reagents、tag peptides、amino Acids、Growth factors等。APExBIO產品質量都伴隨著證書的分析，高效液相色譜，質譜，和HMNR數據。
| R1001-.1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-5 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1001-1 | ARCA EGFP mRNA | Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells. |
| R1005-.1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-5 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1005-1 | Firefly Luciferase mRNA (ARCA, 5mCTP, ψUTP) | Better performance of firefly luciferase mRNA modified by ARCA, 5mCTP and ΨUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1006-.1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-5 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1006-1 | SpCas9 mRNA (ARCA, 5mCTP, ψUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing |
| R1007-.1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-5 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1007-1 | ARCA EGFP mRNA (5-moUTP) | New modification with better performance — direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control to study transfection and expression in mammalian cells. |
| R1008-.1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-5 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1008-1 | ARCA Cy3 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy3 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-.1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-5 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1009-1 | ARCA Cy5 EGFP mRNA (5-moUTP) | 5-moUTP-modified mRNA with better performance, Cy5 tag, direct-detection reporter mRNA that suppresses RNA-mediated innate immune activation, used as control and tool for determining mRNA delivery and localization. |
| R1010-.1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-5 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1010-1 | EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-.1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-5 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1011-1 | EZ Cap™ Cy5 EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP and Cy5-utp, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation |
| R1012-.1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-5 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1012-1 | Firefly Luciferase mRNA (ARCA, 5-moUTP) | Better performance of firefly luciferase mRNA modified by ARCA and 5-moUTP, inhibiting RNA-mediated innate immune activation, stable and efficient expression efficiency, used as an experimental control. |
| R1013-.1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-5 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1013-1 | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) | Firefly Luciferase mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1015-.1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-5 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1015-1 | EZ Cap™ Cas9 mRNA (5-moUTP) | Used with guideRNA for site-specific DNA cleavage in genome editing. |
| R1016-.1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-5 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1016-1 | EZ Cap™ EGFP mRNA (5-moUTP) | EGFP mRNA with Cap 1 structure, modified by 5-moUTP，providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-.1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-5 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1017-1 | EZ Cap™ mCherry mRNA (5mCTP, ψUTP) | mCherry mRNA with Cap 1 structure, modified by 5mCTP/ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1018-.1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-5 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1018-1 | EZ Cap™ Firefly Luciferase mRNA | Firefly Luciferase mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1020-.1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-5 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1020-1 | EZ Cap™ EPO mRNA (ψUTP) | Human erythropoietin (EPO) mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation. |
| R1021-.1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-5 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1021-1 | EZ Cap™ Renilla Luc mRNA | Renilla Luc mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1022-.1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-5 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1022-1 | EZ Cap™ Renilla Luc mRNA (5-moUTP) | Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1023-.1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-5 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1023-1 | EZ Cap™ Human p53 mRNA | Human p53 mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1024-.1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-5 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1024-1 | EZ Cap™ Human p53 mRNA (ψUTP) | Human p53 mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1025-.1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-5 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1025-1 | EZ Cap™ Human PTEN mRNA | Human PTEN mRNA with Cap 1 structure, providing higher transcription efficiency and enhanced stability. |
| R1026-.1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-5 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1026-1 | EZ Cap™ Human PTEN mRNA (ψUTP) | Human PTEN mRNA with Cap 1 structure, modified by ψUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation. |
| R1027-.1 | EZ Cap™ OVA mRNA | Ovalbumin mRNA with Cap 1 structure, providing higher transcription efficiency |
| R1027-5 | EZ Cap™ OVA mRNA | Ovalbumin mRNA with Cap 1 structure, providing higher transcription efficiency |
| R1027-1 | EZ Cap™ OVA mRNA | Ovalbumin mRNA with Cap 1 structure, providing higher transcription efficiency |
| R1028-.1 | EZ Cap™ OVA mRNA (5-moUTP) | Ovalbumin mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation |
| R1028-5 | EZ Cap™ OVA mRNA (5-moUTP) | Ovalbumin mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation |
| R1028-1 | EZ Cap™ OVA mRNA (5-moUTP) | Ovalbumin mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency, and suppressing RNA-mediated innate immune activation |