PARP檢測試劑盒 | 貨號SEA279Hu
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特點
ELISA Kit for Poly ADP Ribose Polymerase (PARP)
PARP檢測試劑盒 (酶聯免疫吸附試驗法) | 貨號SEA279Hu，偵測的靈敏度0.78-50ng/mL、最低靈敏度0.28ng/mL.。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。Cloud-Clone corp提供高靈敏度之ELISA (酵素免疫分析套組/酶聯免疫吸附試驗法)，專門生產研究用檢測試劑的生技公司，於美國研發和生產，產品線包括ELISA Kit、CLIA Kit、Antibody及recombinant protein；ELISA產品系列超過6000種以上，物種齊全包括rat、mouse、rabbit、cavia、human、porcine、bovine、simian等多物種商品。品質保證。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA、高靈敏度設計ELISA。Uncoated ELISA型式；包括Capture Antibody, Detection Antibody、Standard、Streptavidin-HRP、TMB Substrate, 96-well Plate。
PARP檢測試劑盒 (酶聯免疫吸附試驗法)套組內容
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
PARP檢測試劑盒簡單流程
- Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
PARP檢測試劑盒相關之抗體 | 蛋白質 | ELISA | CLIA產品
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA279Hu01 | Recombinant Poly ADP Ribose Polymerase (PARP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA279Hu01 | Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP) | WB; IHC; ICC; IP. |
| MAA279Hu21 | Monoclonal Antibody to Poly ADP Ribose Polymerase (PARP) | WB; IHC; ICC; IP. |
| SEA279Hu | ELISA Kit for Poly ADP Ribose Polymerase (PARP) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA279Hu | CLIA Kit for Poly ADP Ribose Polymerase (PARP) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA279Hu | Magnetic Luminex Assay Kit for Poly ADP Ribose Polymerase (PARP) | Magnetic Luminex Assay for Antigen Detection. |