硝酸鹽還原酶檢測試劑盒 | Nitrate Reductase Microplate Assay Kit BioWorld貨號ASK1014

硝酸還原酶活性可作為預測穀物的生化工具產量和穀物蛋白質產量。硝酸還原酶促進氨基酸茶葉生產。據悉，茶樹噴灑各種微量營養素（如 Zn、Mn 和 B）與 Mo 一起提高了氨基酸含量茶芽和作物產量。該測定以硝酸鹽對硝酸鹽的酶促水解開始還原酶。酶催化反應產物偶氮化合物可測定在 540 nm 比色讀數。

Nitrate Reductase (NR), also known as respiratory nitrate reductase. Eukaryotic nitrate reductases are part of the sulfite oxidase family of molybdoenzymes. They transfer electrons from NADH or NADPH to nitrate. Prokaryotic nitrate reductases belong to the DMSO reductase family of molybdoenzymes and have been classified into three groups, assimilatory nitrate reductases (Nas), respiratory nitrate reductase (Nar), and periplasmic nitrate reductases (Nap). The active site of these enzymes is a Mo ion that is bound to the four thiolate functions of two pterin molecules. The coordination sphere of the Mo is completed by one amino-acid side chain and oxygen and/or sulfur ligands. The exact environment of the Mo ion in certain of these enzymes (oxygen versus sulfur as a sixth molybdenum ligand) is still debated. The Mo is covalently attached to the protein by a cysteine ligand in Nap, and an aspartate in Nar. Nitrate reductase activity can be used as a biochemical tool for predicting grain yield and grain protein production. Nitrate reductase promotes amino acid production in tea leaves. It is reported that tea plants sprayed with various micronutrients (like Zn, Mn and B) along with Mo enhanced the amino acid content of tea shoots and also the crop yield. The assay is initiated with the enzymatic hydrolysis of the nitrate by Nitrate Reductase. The enzyme catalysed reaction products azo-compound can be measured at a colorimetric readout at 540 nm.

Product Name	Nitrate Reductase Microplate Assay Kit
Catalog No.	ASK1014
Reactivity	All
Applications	Functional Assay
Description	Detection and Quantification of Nitrate Reductase Activity.
Alternative Names	EC 1.7.99.4; Nitrate reductase; Respiratory nitrate reductase
CAS Number	37256-45-4
Detection Method	Colorimetric
Sample Types	Tissue extracts, Cell lysate
Storage/Stability	Shipped and store at 4°C for 6 months.

Product Name :
Nitrate Reductase Microplate Assay Kit

Reactivity :
All

Applications :
Functional Assay

Description :
Detection and Quantification of Nitrate Reductase Activity.

Alternative Names :
EC 1.7.99.4; Nitrate reductase; Respiratory nitrate reductase

CAS Number :
37256-45-4

Detection Method :
Colorimetric

即用型IHC Detection System kit  | 牛血清白蛋白粉 (BSA)  | 伊紅 Y 染色緩衝液 | PPOs 多酚氧化酶檢測

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