即用型IHC Detection System kit （Mouse/Rabbit）| BioWorld貨號BD6001

超靈敏檢測系統試劑盒(Super Sensitive Detection System kit, BD6001)是聚合物標記(Polymer detection methods)的最新技術。聚合物檢測方法(Polymer detection methods)已被證明可提供更高的靈敏度。與傳統 IHC 系統相比，這種創新的聚合物技術具有重大優勢。超靈敏檢測系統於使用小鼠(mouse)和兔(rabbit)一抗具有放大信號。由於超靈敏檢測不是基於生物素/抗生物素蛋白的系統，因此消除了由於與內源性生物素分子非特異性結合(nonspecific binding)而導致的背景噪聲(background noise)，該系統消除了由於與內源性生物素(endogenous biotin)非特異性結合而導致的背景噪聲(background noise)。超靈敏檢測系統為用戶提供了一種快速、易於使用且用途廣泛的 IHC 檢測系統。

Product Name	Super Sensitive TM IHC Detection System kit （Mouse/Rabbit）
Catalog No.	BD6001
Classification	IHC Related Reagents
Format	Kit
Contents	A:Hydrogen Peroxide Blocking ReagentB:Blocking ReagentC:Antibody AmplifierD:HRP PolymerE:DAB Substrate ReagentF:DAB Chromogen Reagent
Storage & Shelf Life	Store at 2-8°C. Each component is stable for up to 12 Months
Research use	For research use only, not for use in diagnostic procedures.

Alternative Name :
1. Within the validity period; 2. Not intended to clinical diagnosis.

Classification :
IHC Related Reagents

Format :
Kit

Contents :
A:Hydrogen Peroxide Blocking Reagent

B:Blocking Reagent

C:Antibody Amplifier

D:HRP Polymer

E:DAB Substrate ReagentF:DAB Chromogen Reagent

Storage & Shelf Life :
Store at 2-8°C. Each component is stable for up to 12 Months

Procedure :
1. Deparaffinize and rehydrate tissue section；PBS/TBS wash for 2 min×3； 2. Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS/TBS wash for 2 min×3; 3. Incubate slide in Hydrogen Peroxide Blocking Reagent for 10 minutes, PBS/TBS wash for 2 min×3; 4. Apply Blocking Reagent and incubate for 5 minutes, PBS/TBS wash for 2 min×3; (May be omitted if primary antibodies are diluted in buffers containing normal goat serum.); 5. Apply primary antibody and incubate according to manufacturer\’s recommended protocol，PBS/TBS wash for 2 min×3； 6. Apply HRP Polymer and incubate for 10 min; (NOTE: HRP is light sensitive. Please avoid unnecessary light exposure.)，PBS/TBS wash for 2 min×3； 7. Add 30 μl (1 drop) DAB Chromogen to 1 ml of DAB Substrate, mix by swirling and apply to tissue. Incubate for about 3-5 minutes，PBS/TBS wash for 2 min×3； 8. Counterstain and coverslip using a permanent mounting media.

Research use :
For research use only, not for use in diagnostic procedures.

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