【幹細胞研究】Cultrex Embryoid Body Formation Kit－Trevigen
特點
美國TREVIGEN公司成立於1992年。致力於研發偵測細胞凋亡、DNA斷損與修復、體內血管新生分析套組（In vivo angiogenesis assay, DIVAA）、體外血管新生分析套組（in vitro angiogenesis assay）、慧星分析法（Comet assay Kit）、細胞移行/細胞侵襲（Cell migration and Cell invasion assay）、基底膜萃取物（Basement Membrane Extract）、以及PAR、PARP、PARG所需用到的抗體與試劑。
Trevigen Cultrex® Embryoid Body Formation Kit
相較傳統Embryoid Body (EB)生成法，Trevigen Cultrex® Embryoid Body Formation Kit可生成大小尺寸統一的embryoid bodies (EBs)。已在human pluripotent stem cells (hESCs and iPSCs)測試並有良好的結果發表。
- Cultrex® Embryoid Body Formation Kit（3550-096-K） 優點：
- 形成EB成功率高。
- EB尺寸大小統一。
- 直接在多孔盤中形成EB，不須額外transfer步驟。
- High-throughput形式。
- 內附ROCK抑制劑Y-27632－增加PSC存活率。
- 特製EB Formation Matrix－促進細胞聚集。
A, B, C – 使用 Cultrex® Embryoid Body Formation Kit進行human iPSC EBs formation。 D, E, F – 使用傳統方法進行human iPSCs EBs formation。
- 套組內容物：
- EB Formation Matrix－促進幹細胞聚集形成EB。
- ROCK Inhibitor Y-27632－顯著增加單細胞培養下幹細胞的存活率。
- 96 Well EB Formation Plate－控制EB大小和形狀，並在各個well中形成單一EB。
| 產品型號 | 產品名稱 | 包裝 |
| 3550-096-K | Cultrex® Embryoid Body Formation Kit | 96 samples |
參考文獻：
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- Goh S.K., Olsen P and Banerjee I. 2013. Extracellular matrix aggregates from differentiating embryoid bodies as a scaffold to support ESC proliferation and differentiation. PLoS One. 2013. 8(4):e61856.