β-澱粉樣蛋白前體抗體 | beta-amyloid protein precursor , APP貨號5045-100
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β-澱粉樣蛋白前體（beta-amyloid protein precursor , APP）抗體 | 貨號5045-100
beta-amyloid protein precursor (APP)
產品描述：大腦皮質中澱粉樣蛋白-β肽（amyloid-beta peptide , Abeta）的積累是阿爾茨海默病 (Alzheimer’s disease)發病機制中的關鍵事件。β-澱粉樣蛋白前體（beta-amyloid protein precursor , APP）被β-分泌酶 (beta-secretase) 切割，產生蛋白質的可溶性衍生物和膜錨定的99-氨基酸羧基末端片段（membrane anchored 99-amino acid carboxy-terminal fragment , C99）。 C99片段充當γ-分泌酶(gamma-secretase)的底物以產生4kDa澱粉樣蛋白-β肽（amyloid-beta peptide , Abeta），其沉積在所有阿爾茨海默(Alzheimer’s disease)病患者的腦中。
| Cat # +Size | 5045-100 |
| Size | 100 µg |
| Antibody Target | APP |
| Alternate Name | amyloid-b-peptide, amyloid beta peptide, Amyloid beta A4 protein, Alzheimer disease amyloid protein, ABPP, APPI, Cerebral vascular amyloid peptide, |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG1 |
| Immunogen | A peptide corresponding to the amino acids 737 to 751 of APP or 85 to 99 of the C99 fragment generated by b-secretase cleavage. |
| Accession # | P05067 |
| Gene ID | 351 |
| Appearance | Colorless liquid |
| Concentration | 1 mg/ml |
| Formulation | 100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide. |
| Purification | Affinity chromatography purified via peptide column. |
| Species Reactivity | Human, mouse, rat |
| Application | Western blot, Immunohistochemistry, ELISA |
| Positive Control | Mouse and rat brain tissue lysates |
| Application & Usage | Immunohistochemistry: 2 μg/ml; Western Blot: 1 – 2 μg/ml; ELISA. However, the optimal conditions should be determined individually. |
| Handling | The antibody solution should be gently mixed before use. |
乾浴加熱水浴槽 | Finnpipette F3 微量分注器 | 試管振盪器 Vortex Mixer | Genereach迷你型微量離心機 | ELISA儀器
Ready-to-use IHC kit (Biotin free), One-Step HRP Polymer anti-Mouse, Rat and Rabbit IgG (H+L) with DAB
產品描述：免疫組織化學（Immunohistochemistry , IHC）/免疫細胞化學（Immunocytochemistry , ICC）是通過使用標記的抗體作為特異性試劑，通過抗原 – 抗體相互作用來定位組織切片/細胞中的抗原，抗原 – 抗體相互作用通過諸如螢光染料(fluorescent dye) ，酶(enzyme,) ，放射性元素(radioactive element) 或膠體金的標記物等讓表現可視化。 通常使用幾種IHC技術，例如標記的生物素二抗鏈黴抗生物素蛋白 – 過氧化物酶 (labeled biotin secondary antibody streptavidin-peroxidase, HRP) ，抗HRP-HRP，ABC催化的信號放大，polymer system聚合物系統（一步或兩步）等，以檢測組織和細胞中的抗原。 BioVision的即用型IHC試劑盒採用聚合物技術( polymer system)，可提高靈敏度和檢測靈敏度。 IBSC一步法，針對抗小鼠，大鼠和兔IgG（H + L） polymer，不同於免疫生物素/抗生物素蛋白的系統 (biotin/avidin free system)，可以對膜 (membrane) ，細胞質 (cytoplasmic) 和核抗原(nuclear antigens) 直接進行染色。 它為用戶提供了快速且易於使用的IHC檢測系統。
| Cat # +Size | K405-50 |
| Size | 50 tests |
| Species Reactivity | Mouse, rat, and rabbit |
| Applications | A biotin/avidin free system that stains membrane, cytoplasmic and nuclear antigens. Immunohistochemistry (IHC) and immunocytochemistry (ICC). |
| Features & Benefits | • Simple procedure; takes ~ 3 hours • Fast and convenient • The assay is sensitive and stable • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. |
| Kit Components | • Ready-to-use, peroxidase block, hydrogen peroxide • Ready-to-use protein blocking solutions • Primary antibody dilution buffer • IBSC-one-step HRP-anti-mouse, rat, and rabbit polymer • Reagent BS (ready to use buffer & substrate) • Reagent C (conc. DAB chromogen) |
| Storage Conditions | 2-8°C |
| Shipping Conditions | Gel Pack |