Scrambled sgRNA CRISPR Lentivirus | 貨號K019

即用型Lentivirus提供力價10⁷ IU/ml, 300ul

產品介紹:  Abmgood提供一系列針對不同CRISPR目標序列, 設計sgRNA-CRISPR Lentivirus表現載體。超過90％的構建體將在20-90％的細胞中完全敲除。 大多數構建體> 50％  transduced轉導和selected cells。 越來越多證據表明，sgRNA比shRNA和siRNA敲除機率高。購買sgRNA Vector, 如果設計用於Cas9核酸酶的sgRNA構建體沒有合理的敲除，將免費設計另一組3個構建體。需確保您盡可能地優化了您的實驗，包括（如適用）增加您的MOI（最多10次）和感染持續時間（最多72小時），並在進行clone screening克隆篩選。

於系統長非編碼RNAs (long non-coding RNAs): 該系統可以用於通過使用一對sgRNA敲除長的非編碼RNA或miRNA或任何短片段的DNA。 但是，效率將比敲除編碼蛋白質的基因低得多，約為1％。 因此，需要單細胞克隆。 可能設計3個靶向lncRNA開始和結束的sgRNA（3×2結構）。3×3的組合，用於敲除靶向的lncRNA，PCR篩選鑑定為最佳組合。可以在轉導2個最佳sgRNA後進行單細胞克隆。預計1-5％的克隆中，lncRNA將被完全敲除。

Species	N/A
Vector	pLenti-U6-sgRNA-PGK-Neo
Target Sequence	GCACTCACATCGCTACATCA
Format	Virus
Appearance	Liquid
Storage	Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Shelf Life	1 year (when at -80°C or below in a non-frost free freezer)
Shipping	Shipped with dry ice
Additional Materials	– CRISPR Genomic Cleavage Detection Kit (G932)
Quality Control	Restriction Enzyme Digest and Sequencing

 

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Okugawa, Y et al. “Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer.” Gut : (2015). DOI: 10.1136/gutjnl-2015-309359. PubMed: 26475630.