葡萄糖攝取呈色測定試劑盒 | Glucose Uptake Colorimetric Assay Kit貨號K676

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特點
Non-radioactive, Highly Sensitive Assay-Glucose Uptake Colorimetric Assay Kit
產品介紹: 葡萄糖攝取是研究細胞信號傳導和葡萄糖代謝的重要生物學過程。 2-脫氧葡萄糖(2-DG)與葡萄糖的結構相似已被廣泛應用在葡萄糖攝取之測量方法。 與葡萄糖一樣,2-DG可以被葡萄糖轉運蛋白攝入並代謝成2-DG-6-磷酸(2-DG6P)。 然而,2-DG6P不能進一步代謝,從而積累在細胞中。 累積的2-DG6P與細胞的2-DG(或葡萄糖)攝取成正比。 在BioVision的葡萄糖攝取比色測定試劑盒中,2-DG6P被氧化生成NADPH,可通過酶再循環擴增反應來測定。 這種易於使用的非放射性試劑盒是高度敏感的,可以檢測低至10 pmol /孔的葡萄糖攝取。

| Cat # +Size | K676-100 |
| Size | 100 assays |
| Detection Method | Absorbance (412 nm) |
| Species Reactivity | Mammalian |
| Applications | This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well. |
| Features & Benefits | • Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive |
| Kit Components | • Extraction Buffer • Neutralization Buffer • 2-Deoxyglucose (2-DG, 10 mM) • Assay Buffer • Enzyme Mix (lyophilized) • Recycling Mix (lyophilized) • 2-DG6P Standard (lyophilized) • Glutathione Reductase (lyophilized) • Substrate-DTNB (lyophilized) |
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