****基因合成/Lentivirus****優惠供應
abmgood是過去十年的領先客製化基因合成供應商，在基因庫數據庫中列出的任何基因（包括複合合成基因）的客製化基因合成方面擁有豐富的經驗。合成後的基因可以選擇將任何以下表達載體，如慢病毒和腺病毒表達系統 (此基因合成方案, 限定於架構於AMB表現載體上; 載體優惠請洽當區業務)。為了方便您對成功的基因表達和蛋白質生產的研究，abm為策略性設計的表達載體陣列提供了26,000個人類基因和> 20,000個小鼠和大鼠基因的整個譜庫。abm蛋白表達載體的主要特徵包括：在原核（基於大腸桿菌; T7啟動子）或真核（哺乳動物細胞;基於CMV啟動子）的系統中表達的選擇。選擇5個標籤（His，HA，MBP，GST和D-tag;一些組合），其中包含His標籤的N端和C端選項。下為熱門架構之載體參考
- pLenti-III-HA (CMV Promoter)
- pLenti-III-EF1a
- pLenti-CMV-GFP-2A-Puro
- pLenti-CMV-RFP-2A-Puro
- pLenti-CMV-GFP-2A-Puro
| 基因合成客製化服務 | Description | Cat. No. | Quant. |
| Economy Gene Synthesis & Subcloning | For non-complicated sequences Synthesize up to 950bp – Complimentary subcloning into pUC57, EcoRV site – Sequence verification to confirm full length sequence is generated – Sequence must be approved for economy synthesis by abm |
C078 | 1.0 μg |
| Gene Synthesis | For any sequence up to 4kb in size Genes are fully sequenced Must be ordered with Subcloning Service I (C096) *minimum charge of $148.50 |
C098 | 1.0 μg |
| Subcloning Service I | Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice. End sequencing of the insert is included with this service to confirm proper insertion*. *For full sequencing, please refer to C197. |
C096 | 1.0 μg |
| Subcloning Service II | Subclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are not compatible with the expression vector of choice. Full length DNA sequencing is included for sequence verification. *Genes over 3kb are subject to an additional charge of $150.00/kb (C100). |
C097 | 1.0 μg |
| Site-directed Mutagenesis | Mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector. Full length sequencing to confirm presence of mutation *Fragments over 2kb are subject to an additional charge of $150.00/kb (C100). |
C047 | 1.0 μg |
| Additional Charge for Extra 1kb |
Additional charge for fragments over the stated size limit ($150.00/kb) *Only applicable to C097 and C047. |
C100 | N/A |
| Promoter Change Service | Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1a, PGK, UbC | C126 | 1.0 μg |
| Tag Addition/Removal Service (≤30bp) | Addition/Removal of an N-terminal or C-terminal tag smaller than 30bp | C127 | 1.0 μg |
| Tag Addition/Removal Service (>30bp) | Addition/Removal of an N-terminal or C-terminal tag larger than 30bp *Tags over 1kb are subject to an additional charge of $150.00/kb (C100). |
C128 | 1.0 μg |
| Reporter Addition/Removal Service | Addition, removal or switching of a GFP or RFP reporter in one of abm’s vectors or a vector of your choice. | C129 | 1.0 μg |
| Antibiotic Switching Service | Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice. | C140 | 1.0 μg |
| DNA Sequencing | Verify the sequence and position of your gene of interest or sequence your PCR products. | C199 | Single Reaction |
| DNA Sequencing with Custom Primer Synthesis | Verify the sequence and position of your gene of interest or sequence your PCR products. | C197 | Single Reaction |
| Bacterial Glycerol Stock | Bacterial culture of the custom plasmid. | C139 | 1 Clone |
| Plasmid Amplification (10-20 μg) | Amplification of the custom plasmid. | C309 | 10-20 μg |
| Plasmid Amplification (80-100 μg) | Amplification of the custom plasmid. | C310 | 80-100 μg |
不同標籤快速參考指南：
| Tag Name | Description | Size (kDa) | Cleavable |
| C-His | A single C-terminal 6X-Histidine tag. | 0.84 | No |
| N-His | A single N-terminal 6X-Histidine tag. | 2.3 | Yes (with thrombin) |
| C-HA | A single C-terminal Hemagglutinin tag. | 1.1 | No |
| His-MBP | Dual N-terminal tag; 6X Histidine followed by Maltose Binding Protein. | 43 | Yes (with TEV) |
| His-GST | Dual N-terminal tag; 6X Histidine followed by Glutathione-S-Transferase Protein. | 27.9 | Yes (with TEV) |
| N-D-C-HA | Dual tags; N-terminal 3X D-tag and C-terminal Hemagglutinin tag. | N: 3X FLAG: 3.5 C: HA: 1.1 |
N: Yes (with TEV) C: No |
| N-D-C-His | Dual tags; N-terminal 3X D-tag and C-terminal 6X-Histidine tag. | N: 3X FLAG: 3.5 C: His: 0.84 |
N: Yes (with TEV) C: No |
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| Lentiviral 系列載體之選擇 | Adenoviral 系列載體之選擇 | Protein Expression System系列載體之選擇 |
| pLenti-III-HA | pShuttle (+) | pPM-His |
| pLenti-EF1a-GFP-2A-Puro | pShuttle (-) | pPM-HA |
| pLenti-CMV-GFP-2A-Puro | pShuttle-HA | pPB-His |
| pLenti-CMV-RFP-2A-Puro | pShuttle-His | pPB-His-MBP |
| pLenti-Bi-cistronic | pPromoter | pPB-His GST |
| pLenti-Tri-cistronic | pPM-N-D-C-HA | |
| pLenti-III-EF1α | pPM-N-D-C-His | |
| pLenti-III-PGK | ||
| pLenti-III-UbC | ||
| pLenti-Promoterless | ||
| Additional lentiviral vectors available. | Additional vectors available. | * Vector maps and catalog numbers coming soon. Please contact customer service for more information regarding the above vectors. |